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[192004_at] tubulin alpha chain

Description F32H2.9 /REP_DB=WormBase Gene ID /WP=CE09884 /GEN=tba-6 /TR=P91873 /GB=CAB04246.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=tubulin alpha chain
Cluster 6grp_total > 25      4grp_total > 54      6grp_cgc > 23      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 102
(3)
A 		46.9
(-3)
A 		80
(0.6)
A 		73
(-0.2)
A 		55
B 82.6
(0.3)
A 		118.7
(3)
A 		90.2
(0.9)
A 		38.9
(-3)
A 		80
C 127.4
(3)
A 		73.9
(-1.2)
A 		51.8
(-3)
A 		78.5
(-0.9)
A 		76
avr 104
(3) 		79.8
(-0.6) 		74
(-1.4) 		63.5
(-3) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 95.6
(3)
A 		87.4
(2.5)
A 		45.8
(-0.2)
A 		42.9
(-0.4)
A 		9.5
(-2.6)
A 		2.7
(-3)
A 		93
B 134.8
(3)
A 		60.8
(-0.7)
A 		53.8
(-1.1)
A 		37.1
(-1.9)
A 		15.7
(-3)
A 		48.6
(-1.3)
A 		119
C 97.4
(1.9)
A 		115.7
(3)
A 		74.5
(0.6)
A 		14.2
(-3)
A 		26.9
(-2.2)
A 		27
(-2.2)
A 		102
avr 109.3
(3) 		88
(1.6) 		58
(-0.3) 		31.4
(-2.1) 		17.4
(-3) 		26.1
(-2.4) 		92
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 37.1
(3)
A 		10.2
(-3)
A 		20.9
(-0.6)
A 		10.7
(-2.9)
A 		27
B 26.3
(2.1)
A 		29.3
(3)
A 		20.9
(0.6)
A 		8.3
(-3)
A 		21
C 42.2
(3)
A 		21.3
(-1.3)
A 		18
(-2)
A 		13.2
(-3)
A 		29
avr 35.2
(3) 		20.3
(-0.6) 		19.9
(-0.7) 		10.7
(-3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 17.6
(0.3)
A 		31.1
(3)
A 		10
(-1.2)
A 		10.9
(-1)
A 		2.9
(-2.6)
A 		0.7
(-3)
A 		30
B 46.9
(3)
A 		20.2
(-1)
A 		13.1
(-2)
A 		9.8
(-2.5)
A 		6.4
(-3)
A 		18.1
(-1.3)
A 		41
C 36.1
(3)
A 		35.3
(2.9)
A 		19
(-0.1)
A 		2.9
(-3)
A 		8.7
(-2)
A 		9
(-1.9)
A 		33
avr 33.5
(3) 		28.9
(2) 		14
(-1.3) 		7.9
(-2.6) 		6
(-3) 		9.3
(-2.3) 		28
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192004_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.754
Sequence Type Exemplar sequence
Representative Public ID F32H2.9 NCBI
Target Description F32H2.9 /REP_DB=WormBase Gene ID /WP=CE09884 /GEN=tba-6 /TR=P91873 /GB=CAB04246.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=tubulin alpha chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060018(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060018 NCBI Caenorhabditis elegans TuBulin, Alpha family member (tba-6) (tba-6) mRNA, complete cds. 11/11 None
SNAP00000013734 ENSEMBL cdna:SNAP chromosome:CEL140:I:9002002:9006657:1 11/11 None
GENEFINDER00000013772 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9005592:9006657:1 11/11 None
F32H2.9 ENSEMBL cdna:known chromosome:CEL140:I:9002126:9006717:1 gene:F32H2.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9002698-9006588(+) 100.0 100.0
Public Domain and Genome References
Gene Title tubulin alpha chain
Gene Symbol tba-6
Ensembl F32H2.9
Entrez Gene 172717 Entrez gene
SwissProt P91873 EMBL-EBI
WormBase CE34484 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1601920_AT similar to Tubulin alpha-8 chain (Alpha-tubulin 8) cfa
CANINE_2:CFAAFFX.24911.1.S1_S_AT similar to Tubulin alpha-8 chain (Alpha-tubulin 8) cfa
CHICKEN:GGA.818.1.S1_AT tubulin, alpha 8 gga
HG-U133_PLUS_2:220069_AT tubulin, alpha 8 hs
HG-U133A:220069_AT tubulin, alpha 8 hs
HG-FOCUS:220069_AT tubulin, alpha 8 hs
HG-U133A_2:220069_AT tubulin, alpha 8 hs
U133_X3P:G9507214_3P_AT tubulin, alpha 8 hs
HG-U95C:61935_AT tubulin, alpha 8 hs
MOUSE430_2:1419518_AT tubulin, alpha 8 mm
MOE430A:1419518_AT tubulin, alpha 8 mm
MOUSE430A_2:1419518_AT tubulin, alpha 8 mm
MU11KSUBB:MSA.26512.0_S_AT tubulin, alpha 8 mm
MG-U74AV2:104682_AT tubulin, alpha 8 mm
MU19KSUBB:TC32509_AT Tubulin, alpha 8, mRNA (cDNA clone MGC:28850 IMAGE:4507364) mm
RAE230A:1390766_AT similar to Tubulin alpha-8 chain (Alpha-tubulin 8) rn
RAT230_2:1390766_AT similar to Tubulin alpha-8 chain (Alpha-tubulin 8) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04246 Hypothetical protein F32H2.9 [Caenorhabditis elegans] ref|NP_492419.2| TuBulin, Alpha family member (tba-6) [Caenorhabditis elegans] 0.0
blast CAE67011 Hypothetical protein CBG12412 [Caenorhabditis briggsae] 0.0
blast BAA32601.1 alpha tubulin (tba-6) [Caenorhabditis elegans] 0.0
blast CAB04246 Hypothetical protein F32H2.9 [Caenorhabditis elegans] ref|NP_492419.2| TuBulin, Alpha family member (tba-6) [Caenorhabditis elegans] 1.0E-130
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 9.8E-103
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 3.2E-75
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 9.8E-103
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 3.2E-75
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 5.3E-9
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 3.2E-75
Sequence
Target Sequence 
>C. ELEGANS:192004_AT
aaacaaatctcgttccgtatccacgtattcatttcccattagtcacatatgctccacttg
tatctgctgaacgtgcttctcatgagcagaatacagtctctgatatgacacatgcatgct
ttgaaccagggtatcaaatggtcaagtgtgatccaagaagaggaaaatatatggcagtgt
gcctgctgtatcgtggtgatgttgtaccaaaagatatcaactcagcaattgcagctgtaa
agacaaaacggacagttcaatttgttgaatggtgtccaaccggtttcaaagttggcatca
actatcaaccaccaacagttgttccgggtggagatttgggaaaacagacaagaagtgttt
gtatgatcagtaatactactgctatcgccgaagcatgggctcggctcgatcataaatttg
acttgatgtatgcaaaacgtgcttttgtgcactggtatgttggagaaggaatggaagaag
gcgaattctcagaagccagggaggatatggctgctctcgaaaaagattacgaagagattg
gggaagacgagcttccagatgacattgacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACAAATCTCGTTCCGTATCCACG 383 129 785 Antisense
CGTATTCATTTCCCATTAGTCACAT 507 247 808 Antisense
AGTCACATATGCTCCACTTGTATCT 267 61 825 Antisense
TATCTGCTGAACGTGCTTCTCATGA 427 657 845 Antisense
ACACATGCATGCTTTGAACCAGGGT 650 109 892 Antisense
ATGGCAGTGTGCCTGCTGTATCGTG 334 51 955 Antisense
GAATGGTGTCCAACCGGTTTCAAAG 324 333 1051 Antisense
TCAACCACCAACAGTTGTTCCGGGT 553 611 1089 Antisense
GTAATACTACTGCTATCGCCGAAGC 411 463 1154 Antisense
GCCGAAGCATGGGCTCGGCTCGATC 289 285 1171 Antisense
GACGAGCTTCCAGATGACATTGACG 360 379 1330 Antisense
Sequence Source Affymetrix Proprietary Database