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[192000_s_at]

Description K08B4.1 /REP_DB=WormBase Gene ID /WP=CE25048 /GEN=lag-1 /TR=Q9TYY1 /GB=AAC68978.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=DNA-binding protein
Cluster 6grp_high1_10_10 > 49      4grp_high_8_8 > 34      6grp_total > 39      4grp_total > 45      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 213
1 2 3 4 range
A 337.7
(3)
P 		226.4
(-1.3)
A 		231.3
(-1.1)
A 		181.3
(-3)
M 		156
B 276.3
(3)
P 		267.3
(2.3)
A 		197.3
(-3)
A 		213.9
(-1.7)
P 		79
C 392.5
(3)
P 		287.8
(0)
P 		208.5
(-2.2)
P 		179.8
(-3)
P 		213
avr 335.5
(3) 		260.5
(-0.1) 		212.4
(-2.1) 		191.7
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 291.4
(-0.5)
P 		325.2
(0.4)
P 		198.6
(-3)
M 		308.9
(-0)
P 		314.4
(0.1)
P 		422.7
(3)
P 		224
B 275.6
(0.1)
P 		241.4
(-1.9)
P 		222.7
(-3)
P 		255.1
(-1.1)
P 		326.1
(3)
P 		320.7
(2.7)
P 		103
C 463.6
(3)
P 		211.8
(-3)
P 		273.4
(-1.5)
P 		289.6
(-1.1)
P 		406.1
(1.6)
P 		226.6
(-2.6)
P 		252
avr 343.5
(2.7) 		259.5
(-1.6) 		231.6
(-3) 		284.5
(-0.3) 		348.9
(3) 		323.3
(1.7) 		117
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 123
(3)
P 		49.1
(-1.6)
A 		60.5
(-0.9)
A 		26.5
(-3)
M 		97
B 88.1
(3)
P 		66
(-0.1)
A 		45.7
(-3)
A 		45.6
(-3)
P 		43
C 129.9
(3)
P 		82.7
(0.2)
P 		72.7
(-0.4)
P 		30.3
(-3)
P 		100
avr 113.7
(3) 		65.9
(-0.6) 		59.6
(-1.1) 		34.1
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 53.7
(-2.1)
P 		115.7
(3)
P 		43.3
(-3)
M 		78.3
(-0.1)
P 		94.9
(1.3)
P 		102.9
(1.9)
P 		72
B 95.9
(0.2)
P 		80.3
(-1)
P 		54.1
(-3)
P 		67.6
(-2)
P 		132.5
(3)
P 		119.6
(2)
P 		78
C 171.8
(3)
P 		64.6
(-2.7)
P 		69.6
(-2.4)
P 		58.2
(-3)
P 		131.4
(0.9)
P 		75.9
(-2.1)
P 		114
avr 107.1
(1.8) 		86.9
(-0.1) 		55.7
(-3) 		68
(-1.8) 		119.6
(3) 		99.5
(1.1) 		64
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192000_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10509
Sequence Type Exemplar sequence
Representative Public ID K08B4.1 NCBI
Target Description K08B4.1 /REP_DB=WormBase Gene ID /WP=CE25048 /GEN=lag-1 /TR=Q9TYY1 /GB=AAC68978.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=DNA-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171350(11), NM_001028107(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171350 NCBI Caenorhabditis elegans Lin-12 And Glp-1 phenotype family member (lag-1) (lag-1) mRNA, complete cds. 11/11 A 
NM_001028107 NCBI Caenorhabditis elegans Lin-12 And Glp-1 phenotype family member (lag-1) (lag-1) mRNA, complete cds. 11/11 None
SNAP00000036221 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6108024:6110098:1 11/11 None
GENEFINDER00000036228 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6108114:6109604:1 9/11 None
K08B4.1a ENSEMBL cdna:known chromosome:CEL140:IV:6095395:6110623:1 gene:K08B4.1 11/11 None
K08B4.1b ENSEMBL cdna:known chromosome:CEL140:IV:6095396:6110098:1 gene:K08B4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6095404-6110104(+) 100.0 100.0
Public Domain and Genome References
Ensembl K08B4.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC68978 Lin-12 and glp-1 phenotype protein 1, isoform a [Caenorhabditis elegans] ref|NP_741410.1| Lin-12 And Glp-1 phenotype family member (lag-1) [Caenorhabditis elegans] gb|AAB03859.1| DNA-binding protein LAG-1 gb|AAB03858.1| lag-1 gene product 0.0
blast AAM98006 Lin-12 and glp-1 phenotype protein 1, isoform b [Caenorhabditis elegans] ref|NP_001023278.1| Lin-12 And Glp-1 phenotype family member (lag-1) [Caenorhabditis elegans] 0.0
blast 1TTU Chain , Crystal Structure Of Csl Bound To Dna 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 1.9E-11
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 1.9E-11
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 1.9E-11
Pfam IPR002909 EMBL-EBI Cell surface receptor IPT/TIG 1.1E-5
Sequence
Target Sequence 
>C. ELEGANS:192000_S_AT
aaccagtctctcaacttcacaaatgtgcattccaaatgattgacaatgagctcgtctatc
tttgtctcagtcatgacaagattattcaacatcaagccacagctatcaatgagcatcgtc
atcagatcaacgatggagctgcgtggacaatcatctcgactgataaggctgaatatcgat
tcttcgaagcgatgggacaggttgcgaacccgatttctccatgtccagttgtcggttcac
ttgaagttgatggacatggtgaagcgtcgcgtgtcgagttgcatggaagagattttaagc
caaatctgaaagtttggtttggagcaaccccggttgaaacgacgttcagaagtgaagaga
gtcttcattgttcgataccacctgtgagtcaggttcgaaatgagcaaactcactggatgt
ttacgaataggacaaccggcgatgttgaggttccaatttcattggtacgagatgatggtg
tcgtctactcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCAGTCTCTCAACTTCACAAATG 244 147 1469 Antisense
TGAGCTCGTCTATCTTTGTCTCAGT 607 567 1515 Antisense
ATTATTCAACATCAAGCCACAGCTA 372 13 1549 Antisense
GCCACAGCTATCAATGAGCATCGTC 509 277 1564 Antisense
GGAGCTGCGTGGACAATCATCTCGA 453 521 1603 Antisense
TGGGACAGGTTGCGAACCCGATTTC 614 541 1661 Antisense
ATGTCCAGTTGTCGGTTCACTTGAA 628 47 1689 Antisense
TGAAGCGTCGCGTGTCGAGTTGCAT 94 575 1728 Antisense
TTCATTGTTCGATACCACCTGTGAG 227 687 1832 Antisense
GGACAACCGGCGATGTTGAGGTTCC 295 515 1898 Antisense
GAGATGATGGTGTCGTCTACTCGTC 203 395 1937 Antisense
Sequence Source Affymetrix Proprietary Database