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[191995_at]

Description F08B1.2 /REP_DB=WormBase Gene ID /WP=CE27920 /GEN=gcy-12 /TR=Q19187 /GB=AAK68301.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster 6grp_total > 19      4grp_total > 20      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 34
(3)
A 		18.5
(-1.9)
A 		14.9
(-3)
A 		22.7
(-0.5)
A 		19
B 27.6
(-2.2)
A 		66.4
(2.4)
A 		21
(-3)
A 		71.7
(3)
A 		51
C 43.3
(-1.4)
A 		24.2
(-2.5)
A 		16.6
(-3)
A 		113.9
(3)
A 		97
avr 35
(-1) 		36.4
(-0.8) 		17.5
(-3) 		69.4
(3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 65.6
(1.7)
A 		19.4
(-2.9)
A 		18.3
(-3)
A 		78.9
(3)
A 		78.7
(3)
A 		64.8
(1.6)
A 		61
B 43.3
(1.1)
A 		14.8
(-2.7)
A 		58
(3)
A 		37.4
(0.3)
A 		37.6
(0.3)
A 		12.5
(-3)
A 		46
C 47.4
(3)
A 		7.5
(-2.6)
A 		15.3
(-1.5)
A 		4.8
(-3)
A 		13.2
(-1.8)
A 		7.1
(-2.7)
A 		43
avr 52.1
(3) 		13.9
(-3) 		30.5
(-0.4) 		40.4
(1.2) 		43.2
(1.6) 		28.1
(-0.8) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 12.4
(3)
A 		4
(-2.5)
A 		3.9
(-2.6)
A 		3.3
(-3)
A 		9
B 8.8
(-1)
A 		16.4
(3)
A 		4.9
(-3)
A 		15.3
(2.4)
A 		12
C 14.3
(0.8)
A 		7
(-2.5)
A 		5.8
(-3)
A 		19.2
(3)
A 		13
avr 11.8
(2.4) 		9.1
(0.3) 		4.9
(-3) 		12.6
(3) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 12.1
(-0.5)
A 		6.9
(-2.1)
A 		4
(-3)
A 		20
(1.9)
A 		23.7
(3)
A 		15.8
(0.6)
A 		20
B 15.1
(2.9)
A 		4.9
(-2.9)
A 		14.1
(2.3)
A 		9.9
(-0.1)
A 		15.3
(3)
A 		4.7
(-3)
A 		11
C 17.6
(3)
A 		2.3
(-2.5)
A 		3.9
(-2)
A 		1
(-3)
A 		4.3
(-1.8)
A 		2.4
(-2.5)
A 		17
avr 14.9
(3) 		4.7
(-3) 		7.3
(-1.5) 		10.3
(0.3) 		14.4
(2.7) 		7.6
(-1.3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191995_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4479
Sequence Type Exemplar sequence
Representative Public ID F08B1.2 NCBI
Target Description F08B1.2 /REP_DB=WormBase Gene ID /WP=CE27920 /GEN=gcy-12 /TR=Q19187 /GB=AAK68301.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062595(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062595 NCBI Caenorhabditis elegans F08B1.3 (F08B1.3) mRNA, complete cds. 11/11 None
SNAP00000014874 ENSEMBL cdna:SNAP chromosome:CEL140:II:5312090:5319053:1 11/11 None
GENEFINDER00000014880 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5312090:5319053:1 11/11 None
F08B1.3 ENSEMBL cdna:known chromosome:CEL140:II:5317381:5319053:1 gene:F08B1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5312087-5319051(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F08B1.3
Entrez Gene 184170 Entrez gene
SwissProt Q95ZW4 EMBL-EBI
WormBase CE27921 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68302 Hypothetical protein F08B1.3 [Caenorhabditis elegans] ref|NP_494996.1| F08B1.3 [Caenorhabditis elegans] 0.0
blast T15968 hypothetical protein F08B1.2 - Caenorhabditis elegans 0.0
blast AAK68301 Guanylyl cyclase protein 12 [Caenorhabditis elegans] ref|NP_494995.1| Guanylyl CYclase family member (gcy-12) [Caenorhabditis elegans] 0.0
blast CAE59078 Hypothetical protein CBG02368 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-23
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.9E-83
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 1.1E-22
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000014874 1 594-616
GENEFINDER00000014880 1 594-616
Sequence
Target Sequence 
>C. ELEGANS:191995_AT
acggctgtgtcaccaagatcttcgtgtagagcattggatcccactatgaaacttgccgct
cgtgcttcaagtcctgatgagattattttccaggaagacgacgaagatgcactcattgat
aacgactccttattaacatcaaatggaaattcaaaaagtgaagatgttctgagaacatgt
ccccaacctagaagaaaaaacaagcactcattcctccgagacccatctcctctagcaaaa
cgattccgagacgcgtcgccatttggaaagaaaaaaccattttggaattcgaacaaaagc
aacgaacacacgtcttcgccagcagactcaatttcacgacttttccgacgattccgtggt
ggaagtgtcaatgagtacgctgatttgaatcattattgcgacgatgaggatgagagtggt
agagcaaatggttatgagatgcaggagatggggaaaatgactggaaggcaaagagagcaa
agaaatggatcgagaactaaccgttcggtctcgtgctctccggatgaatgtggaactcta
acagacagttcggatcctcatct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGGCTGTGTCACCAAGATCTTCGT 312 95 4429 Antisense
CCACTATGAAACTTGCCGCTCGTGC 537 273 4469 Antisense
CGCTCGTGCTTCAAGTCCTGATGAG 670 255 4485 Antisense
ACCCATCTCCTCTAGCAAAACGATT 337 91 4649 Antisense
CAAAACGATTCCGAGACGCGTCGCC 216 191 4664 Antisense
AAAGCAACGAACACACGTCTTCGCC 279 115 4724 Antisense
GCCAGCAGACTCAATTTCACGACTT 678 279 4746 Antisense
CACGACTTTTCCGACGATTCCGTGG 625 199 4763 Antisense
AGAACTAACCGTTCGGTCTCGTGCT 336 71 4921 Antisense
TCTCGTGCTCTCCGGATGAATGTGG 258 611 4937 Antisense
TAACAGACAGTTCGGATCCTCATCT 438 639 4967 Antisense
Sequence Source Affymetrix Proprietary Database