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[191983_s_at] long-chain enoyl-CoA hydratase

Description C29F3.1 /REP_DB=WormBase Gene ID /WP=CE08435 /TR=O17612 /GB=CAB02799.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=long-chain enoyl-CoA hydratase
Cluster 6grp_total > 47      4grp_total > 48      6grp_cgc > 26      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 64
1 2 3 4 range
A 39.7
(0.5)
A 		36.1
(-0.3)
A 		51.1
(3)
A 		23.5
(-3)
A 		28
B 73.4
(3)
A 		37.3
(-0.9)
A 		40.1
(-0.6)
A 		17.6
(-3)
A 		56
C 9.8
(-3)
A 		37.8
(-0.2)
A 		51.2
(1.1)
P 		70.2
(3)
A 		60
avr 41
(-0.8) 		37.1
(-3) 		47.5
(3) 		37.1
(-3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 56
1 2 3 4 5 6 range
A 44.5
(-1.6)
A 		65
(3)
P 		38.4
(-3)
P 		49.9
(-0.4)
P 		42.4
(-2.1)
P 		54.9
(0.7)
P 		27
B 67.8
(2.1)
A 		44
(-0.5)
P 		20.5
(-3)
A 		32.6
(-1.7)
A 		24.3
(-2.6)
A 		76.6
(3)
P 		56
C 58.1
(-0.7)
M 		56.6
(-1.3)
P 		60
(0.1)
P 		67.1
(3)
P 		52.4
(-3)
P 		58.2
(-0.6)
P 		15
avr 56.8
(1.4) 		55.2
(1) 		39.6
(-3) 		49.9
(-0.4) 		39.7
(-3) 		63.2
(3) 		24
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 14.4
(3)
A 		7.8
(-0.6)
A 		13.3
(2.4)
A 		3.4
(-3)
A 		11
B 23.4
(3)
A 		9.2
(-1.3)
A 		9.3
(-1.3)
A 		3.7
(-3)
A 		20
C 3.3
(-3)
A 		10.9
(0.1)
A 		17.8
(3)
P 		11.8
(0.5)
A 		15
avr 13.7
(3) 		9.3
(-0.6) 		13.5
(2.8) 		6.3
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 8.2
(-3)
A 		23.1
(3)
P 		8.4
(-2.9)
P 		12.7
(-1.2)
P 		12.8
(-1.1)
P 		13.4
(-0.9)
P 		15
B 23.6
(1.7)
A 		14.7
(-0.5)
P 		5
(-3)
A 		8.6
(-2.1)
A 		9.9
(-1.8)
A 		28.6
(3)
P 		24
C 21.6
(3)
M 		17.3
(-0.2)
P 		15.3
(-1.7)
P 		13.5
(-3)
P 		17
(-0.4)
P 		19.5
(1.4)
P 		8
avr 17.8
(1.5) 		18.4
(1.8) 		9.6
(-3) 		11.6
(-1.9) 		13.2
(-1) 		20.5
(3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191983_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12642
Sequence Type Exemplar sequence
Representative Public ID C29F3.1 NCBI
Target Description C29F3.1 /REP_DB=WormBase Gene ID /WP=CE08435 /TR=O17612 /GB=CAB02799.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=long-chain enoyl-CoA hydratase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074409(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074409 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-1) (ech-1) mRNA, complete cds. 11/11 A 
SNAP00000013693 ENSEMBL cdna:SNAP chromosome:CEL140:V:15360378:15363324:-1 11/11 None
GENEFINDER00000013695 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15360378:15363324:-1 11/11 None
C29F3.1 ENSEMBL cdna:known chromosome:CEL140:V:15360282:15363324:-1 gene:C29F3.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15360377-15363324(-) 100.0 100.0
Public Domain and Genome References
Gene Title long-chain enoyl-CoA hydratase
Gene Symbol ech-1
Ensembl C29F3.1
Entrez Gene 180037 Entrez gene
SwissProt O17612 EMBL-EBI
WormBase CE08435 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6631 fatty acid metabolism inferred from electronic annotation QuickGO AmiGO
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02799 Hypothetical protein C29F3.1 [Caenorhabditis elegans] ref|NP_506810.1| Enoyl-CoA Hydratase family member (ech-1) [Caenorhabditis elegans] 0.0
blast NP_491790 T08B2.7b [Caenorhabditis elegans] gb|AAK21464.1| Hypothetical protein T08B2.7b [Caenorhabditis elegans] 0.0
blast NP_491789 T08B2.7a [Caenorhabditis elegans] gb|AAK21462.1| Hypothetical protein T08B2.7a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 2.9E-36
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 8.2E-6
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 6.4E-58
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 3.9E-80
Sequence
Target Sequence 
>C. ELEGANS:191983_S_AT
tccgtctcctccaggaaggtgtcgaaccatctgaacttgacaaactcacaacgaaattcg
gattccctgtgggagctgcgacgttggccgacgaagctggactcgacgtcgcagaacacg
tggctcgatatctcggaaccgctttaggtccacgtgttcatggaggatcggttgatttgt
tgtccgaattggtgcgcgctggacataaggggcgtaagacttcaaagggaatctttgtgt
acggagatggggcaaaaggcagcaagaaagtaaaccaagaggcggcaaagctgtttgaaa
agtataaattgacaccaatcaaatctgtctcatctcctgaggaccgtcaacttcgtctcg
tctctcgatttgtcaatgaggcacttctttgtcttgaagaaggtgttattagctcaccat
ctgacggagatatagcctctgtatttggtctcggttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGTCTCCTCCAGGAAGGTGTCGA 383 593 1661 Antisense
CGAACCATCTGAACTTGACAAACTC 303 253 1683 Antisense
TCGGATTCCCTGTGGGAGCTGCGAC 419 605 1718 Antisense
GGCCGACGAAGCTGGACTCGACGTC 396 543 1746 Antisense
GACTCGACGTCGCAGAACACGTGGC 347 375 1760 Antisense
GAACACGTGGCTCGATATCTCGGAA 322 351 1774 Antisense
TATCTCGGAACCGCTTTAGGTCCAC 450 657 1789 Antisense
CCGCTTTAGGTCCACGTGTTCATGG 519 261 1799 Antisense
TTGGTGCGCGCTGGACATAAGGGGC 569 701 1849 Antisense
ATGAGGCACTTCTTTGTCTTGAAGA 488 45 2036 Antisense
TAGCCTCTGTATTTGGTCTCGGTTT 550 651 2093 Antisense
Sequence Source Affymetrix Proprietary Database