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[191982_s_at] cAMP-dependant protein kinase

Description M03C11.1 /REP_DB=WormBase Gene ID /WP=CE03492 /TR=Q21483 /GB=CAA88953.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=cAMP-dependant protein kinase
Cluster 6grp_high1_10_10 > 06      6grp_total > 06      4grp_total > 22      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 299
1 2 3 4 range
A 374.8
(3)
P 		171.5
(-1.2)
A 		84
(-3)
A 		310.6
(1.7)
P 		291
B 336.3
(3)
P 		185.9
(-0.8)
A 		101.1
(-3)
A 		329
(2.8)
P 		235
C 359.9
(2.4)
P 		194.1
(-1.6)
A 		136.7
(-3)
A 		382.6
(3)
P 		246
avr 357
(3) 		183.8
(-1.2) 		107.3
(-3) 		340.7
(2.6) 		250

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 304
1 2 3 4 5 6 range
A 361.3
(3)
P 		262.9
(0.6)
P 		224.8
(-0.3)
A 		189.2
(-1.1)
P 		124.5
(-2.7)
P 		110.2
(-3)
P 		251
B 381
(3)
P 		253.4
(0)
P 		178.9
(-1.7)
P 		164.9
(-2.1)
P 		149.2
(-2.4)
P 		124.6
(-3)
A 		256
C 330.5
(3)
P 		216.3
(0.3)
P 		225
(0.5)
P 		181.7
(-0.5)
P 		140
(-1.5)
P 		77.1
(-3)
P 		253
avr 357.6
(3) 		244.2
(0.3) 		209.6
(-0.5) 		178.6
(-1.2) 		137.9
(-2.2) 		104
(-3) 		254
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 136.5
(3)
P 		37.2
(-2.2)
A 		21.9
(-3)
A 		45.5
(-1.8)
P 		115
B 107.2
(3)
P 		45.9
(-1.4)
A 		23.4
(-3)
A 		70.1
(0.3)
P 		84
C 119.1
(3)
P 		55.8
(-2.3)
A 		47.6
(-3)
A 		64.5
(-1.6)
P 		72
avr 120.9
(3) 		46.3
(-2) 		31
(-3) 		60
(-1.1) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 66.6
(0.6)
P 		93.5
(3)
P 		48.9
(-1)
A 		48
(-1.1)
P 		37.6
(-2)
P 		26.8
(-3)
P 		67
B 132.6
(3)
P 		84.3
(-0.2)
P 		43.4
(-3)
P 		43.7
(-3)
P 		60.6
(-1.8)
P 		46.5
(-2.8)
A 		89
C 122.5
(3)
P 		66
(-0.5)
P 		57.3
(-1)
P 		36.5
(-2.3)
P 		45.3
(-1.8)
P 		25.8
(-3)
P 		97
avr 107.2
(3) 		81.3
(0.9) 		49.9
(-1.6) 		42.7
(-2.2) 		47.8
(-1.8) 		33
(-3) 		74
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191982_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6561
Sequence Type Exemplar sequence
Representative Public ID M03C11.1 NCBI
Target Description M03C11.1 /REP_DB=WormBase Gene ID /WP=CE03492 /TR=Q21483 /GB=CAA88953.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=cAMP-dependant protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066893(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066893 NCBI Caenorhabditis elegans M03C11.1 (M03C11.1) mRNA, complete cds. 11/11 A 
GENEFINDER00000000557 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10392753:10395955:-1 10/11 None
SNAP00000000514 ENSEMBL cdna:SNAP chromosome:CEL140:III:10393347:10395438:-1 11/11 None
M03C11.1 ENSEMBL cdna:known chromosome:CEL140:III:10393275:10395959:-1 gene:M03C11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10393342-10395939(-) 100.0 100.0
Public Domain and Genome References
Gene Title cAMP-dependant protein kinase
Ensembl M03C11.1
Entrez Gene 176456 Entrez gene
SwissProt Q21483 EMBL-EBI
WormBase CE03492 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597354_AT similar to serine/threonine kinase 32B cfa
CANINE_2:CFA.8447.1.A1_AT similar to serine/threonine kinase 32B cfa
CANINE_2:CFAAFFX.24090.1.S1_AT similar to serine/threonine kinase 32B cfa
CANINE_2:CFAAFFX.24090.1.S1_S_AT similar to serine/threonine kinase 32B cfa
CHICKEN:GGA.11676.1.S1_S_AT serine/threonine kinase 32B gga
CHICKEN:GGAAFFX.24572.1.S1_AT serine/threonine kinase 32B gga
CHICKEN:GGAAFFX.9537.1.S1_S_AT serine/threonine kinase 32B gga
HG-U133_PLUS_2:219686_AT serine/threonine kinase 32B hs
HG-U133A:219686_AT serine/threonine kinase 32B hs
HG-FOCUS:219686_AT serine/threonine kinase 32B hs
HG-U133A_2:219686_AT serine/threonine kinase 32B hs
HU35KSUBC:RC_W72870_AT serine/threonine kinase 32B hs
U133_X3P:G8923753_3P_AT serine/threonine kinase 32B hs
HG-U95B:51856_AT serine/threonine kinase 32B hs
HG-U133_PLUS_2:240679_AT Serine/threonine kinase 32B hs
HG-U133B:240679_AT Serine/threonine kinase 32B hs
HG-U95C:49543_AT Serine/threonine kinase 32B hs
HG-U95D:74216_AT Serine/threonine kinase 32B hs
U133_X3P:HS.232204.0.A1_3P_AT Serine/threonine kinase 32B hs
MOE430B:1431236_AT serine/threonine kinase 32B mm
MOUSE430_2:1431236_AT serine/threonine kinase 32B mm
MOE430A:1452420_AT serine/threonine kinase 32B mm
MOUSE430_2:1452420_AT serine/threonine kinase 32B mm
MOUSE430A_2:1452420_AT serine/threonine kinase 32B mm
MU11KSUBB:MSA.15267.0_S_AT serine/threonine kinase 32B mm
MG-U74CV2:131549_AT serine/threonine kinase 32B mm
MG-U74BV2:109434_AT Serine/threonine kinase 32B, mRNA (cDNA clone MGC:67312 IMAGE:5715729) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88953 Hypothetical protein M03C11.1 [Caenorhabditis elegans] ref|NP_499294.1| M03C11.1 [Caenorhabditis elegans] 0.0
blast CAE71477 Hypothetical protein CBG18395 [Caenorhabditis briggsae] 0.0
blast CAA88953 Hypothetical protein M03C11.1 [Caenorhabditis elegans] ref|NP_499294.1| M03C11.1 [Caenorhabditis elegans] 1.0E-175
blast CAE71477 Hypothetical protein CBG18395 [Caenorhabditis briggsae] 1.0E-167
blast AAH58412 Serine/threonine kinase 32B [Mus musculus] gb|AAH56396.1| Serine/threonine kinase 32B [Mus musculus] gb|AAH52404.1| Serine/threonine kinase 32B [Mus musculus] ref|NP_071861.1| serine/threonine kinase 32B [Mus musculus] 2.0E-93
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-63
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 4.0E-58
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 2.0E-74
hanks 1.4.5 AGC group; AGC IV GPCR kinases; IT11 1.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-79
Pfam IPR000719 EMBL-EBI Protein kinase 1.5E-61
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-79
Sequence
Target Sequence 
>C. ELEGANS:191982_S_AT
ctggcgacacagctggaggatgatcagcttgccacgtcttactcaggcacacggccctac
atggctccggagatttatgcgacttatttggagatagaagacggatatgacagtcgagtt
gattggtgggctcttggtgtgtgcttttatgaaatgctgcgaggaaggacaccgtttgag
tttagctccaggacgaagcccgaggaagcgtacgtagcattccgggagtcatcgattcct
tatcctgcacactggccaactgatctcattcaatttataaattcgatgctgaaattcgac
aaggaaaagcgtctagtcggtttggaagccatcaagaaacacagttacacagaacgtata
gatttcaagagtgtttttgagaaaaaaccatcgccagtgttcattccttgtaaagagggt
ctcaactgtgatccgatgtatgagttggaagaacgtattcttgtctcaacaccaattcat
cggcggagaaccaatcataataatagctcagggagaagctcgagtgagccgcaaaatgcc
gcgttggtcgaggtttcaaaggcgttcattgatttttcccgccataacgtcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGGCGACACAGCTGGAGGATGATC 381 241 526 Antisense
ATGGCTCCGGAGATTTATGCGACTT 541 51 586 Antisense
TGATTGGTGGGCTCTTGGTGTGTGC 684 559 645 Antisense
GGTGTGTGCTTTTATGAAATGCTGC 19 501 661 Antisense
GAAGGACACCGTTTGAGTTTAGCTC 428 333 689 Antisense
GAGTTTAGCTCCAGGACGAAGCCCG 298 381 703 Antisense
CGTACGTAGCATTCCGGGAGTCATC 197 247 734 Antisense
TCCGGGAGTCATCGATTCCTTATCC 153 595 746 Antisense
CACTGGCCAACTGATCTCATTCAAT 394 199 775 Antisense
GCGTCTAGTCGGTTTGGAAGCCATC 90 291 834 Antisense
TTGATTTTTCCCGCCATAACGTCAA 423 705 1094 Antisense
Sequence Source Affymetrix Proprietary Database