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[191981_at] mechanosensory protein like

Description T28F4.2 /REP_DB=WormBase Gene ID /WP=CE06524 /TR=SW:Q22851 /GB=CAA96693.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=mechanosensory protein like
Cluster 6grp_total > 23      4grp_total > 47      6grp_cgc > 31      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 118
1 2 3 4 range
A 67.1
(3)
A 		19.3
(-1.9)
A 		12.7
(-2.6)
A 		8.5
(-3)
A 		59
B 107.8
(3)
A 		4.4
(-3)
A 		6.6
(-2.9)
A 		21.3
(-2)
A 		103
C 122.8
(3)
A 		59
(-2.3)
A 		53.3
(-2.7)
A 		50.1
(-3)
A 		73
avr 99.2
(3) 		27.6
(-2.7) 		24.2
(-3) 		26.6
(-2.8) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 121.6
(3)
A 		113.4
(2.5)
P 		92.2
(1.2)
A 		42.9
(-1.9)
A 		45.5
(-1.7)
A 		25.3
(-3)
A 		96
B 123.8
(3)
P 		88.1
(1.2)
P 		59.2
(-0.3)
M 		69.6
(0.3)
A 		51.8
(-0.7)
A 		5.5
(-3)
A 		118
C 133.5
(3)
A 		125.7
(2.6)
P 		117
(2.1)
P 		68.9
(-0.4)
A 		67.6
(-0.4)
A 		18.1
(-3)
A 		115
avr 126.3
(3) 		109.1
(2.1) 		89.5
(1) 		60.5
(-0.6) 		55
(-0.9) 		16.3
(-3) 		110
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 24.4
(3)
A 		4.2
(-2.2)
A 		3.3
(-2.5)
A 		1.2
(-3)
A 		23
B 34.4
(3)
A 		1.1
(-3)
A 		1.5
(-2.9)
A 		4.5
(-2.4)
A 		33
C 40.7
(3)
A 		17
(-1.4)
A 		18.6
(-1.1)
A 		8.4
(-3)
A 		32
avr 33.2
(3) 		7.4
(-2.4) 		7.8
(-2.3) 		4.7
(-3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 22.4
(-0.1)
A 		40.3
(3)
P 		20.1
(-0.6)
A 		10.9
(-2.2)
A 		13.7
(-1.7)
A 		6.2
(-3)
A 		34
B 43.1
(3)
P 		29.3
(1)
P 		14.4
(-1.2)
M 		18.4
(-0.6)
A 		21
(-0.2)
A 		2
(-3)
A 		41
C 49.5
(3)
A 		38.4
(1.5)
P 		29.8
(0.3)
P 		13.8
(-1.9)
A 		21.9
(-0.8)
A 		6.1
(-3)
A 		43
avr 38.3
(3) 		36
(2.6) 		21.4
(-0) 		14.4
(-1.3) 		18.9
(-0.5) 		4.8
(-3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191981_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2282
Sequence Type Exemplar sequence
Representative Public ID T28F4.2 NCBI
Target Description T28F4.2 /REP_DB=WormBase Gene ID /WP=CE06524 /TR=SW:Q22851 /GB=CAA96693.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=mechanosensory protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059698(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059698 NCBI Caenorhabditis elegans Acid-sensing/Amiloride-Sensitive Ion Channel family member (asic-2) (asic-2) mRNA, complete cds. 11/11 None
GENEFINDER00000023981 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7488263:7491520:1 10/11 None
SNAP00000023975 ENSEMBL cdna:SNAP chromosome:CEL140:I:7488636:7491520:1 11/11 None
T28F4.2 ENSEMBL cdna:known chromosome:CEL140:I:7488263:7491520:1 gene:T28F4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7488193-7491451(+) 100.0 100.0
Public Domain and Genome References
Gene Title mechanosensory protein like
Gene Symbol asic-2
Ensembl T28F4.2
Entrez Gene 189058 Entrez gene
SwissProt Q22851 EMBL-EBI
WormBase CE06524 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA96693 Hypothetical protein T28F4.2 [Caenorhabditis elegans] ref|NP_492099.1| Acid-sensing/Amiloride-Sensitive Ion Channel family member (asic-2) [Caenorhabditis elegans] sp|Q22851|DEGW_CAEEL Degenerin-like protein T28F4.2 in chromosome I 0.0
blast CAE66559 Hypothetical protein CBG11871 [Caenorhabditis briggsae] 0.0
blast CAB03443 Hypothetical protein T28B8.5 [Caenorhabditis elegans] ref|NP_492230.2| T28B8.5 [Caenorhabditis elegans] 4.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492099.1 1 35-57
T28F4.2 1 35-57
GENEFINDER00000023981 1 35-57
Sequence
Target Sequence 
>C. ELEGANS:191981_AT
aaatcaaacaagttccactccgaatattgatttattagttgaatgcaattgtaatcctcc
ctgtgatgaatcaacctacacaccaacagtatctcttgctcaatttccatccactagtta
ttacgtcgcaacttcgtctactgctggggttggaagctgctcttcaacaaactcgaagtt
ttcaagcaaatcagattgtcagaaatggtacaacaataatggaatgattatacaagtgtt
tttggaaacgctcagctacgagctatacactgaaactgcaggatatacggttagcaacgt
tattaatgacttgggaggacaagccggtttgtggcttggactatccgttatttctgttgt
tgagatgacaggtttaatgttagtcatgggtgcgttctgtgtaactggtggagctatcaa
aatggctccagacgatgatgaaattgaaaatgatcacagaatcaaggacgtggaagatgt
gaaaaaggaaatcgatcatttggagaaaaaacacggagaaatggagagcggcagcgatgg
ggaagttgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCAAACAAGTTCCACTCCGAAT 674 113 1059 Antisense
TGAATGCAATTGTAATCCTCCCTGT 414 575 1098 Antisense
ATGAATCAACCTACACACCAACAGT 63 45 1124 Antisense
ACAGTATCTCTTGCTCAATTTCCAT 339 107 1144 Antisense
TTCCATCCACTAGTTATTACGTCGC 126 701 1163 Antisense
TATTACGTCGCAACTTCGTCTACTG 595 661 1177 Antisense
TACTGCTGGGGTTGGAAGCTGCTCT 455 639 1197 Antisense
GCCGGTTTGTGGCTTGGACTATCCG 70 283 1381 Antisense
GGACTATCCGTTATTTCTGTTGTTG 232 525 1396 Antisense
GTTAGTCATGGGTGCGTTCTGTGTA 114 445 1437 Antisense
GAGCGGCAGCGATGGGGAAGTTGAC 516 385 1584 Antisense
Sequence Source Affymetrix Proprietary Database