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[191979_at]

Description C09G4.2 /REP_DB=WormBase Gene ID /WP=CE03979 /TR=Q9GYS0 /GB=AAF99865.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cAMP-dependant protein kinase
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 97      4grp_highest_8_8 > 14      4grp_high_8_8 > 14      6grp_highest_nonorm > 78      6grp_total > 96      4grp_total > 17      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2397
1 2 3 4 range
A 809.9
(-3)
P 		1186.1
(-2)
P 		1095.3
(-2.3)
P 		3136.8
(3)
P 		2327
B 771.1
(-3)
P 		1232.8
(-1.8)
P 		1212
(-1.9)
P 		3155.8
(3)
P 		2385
C 759.1
(-3)
P 		1272.9
(-1.6)
P 		1179
(-1.9)
P 		3010.6
(3)
P 		2252
avr 780
(-3) 		1230.6
(-1.8) 		1162.1
(-2) 		3101.1
(3) 		2321

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 895
1 2 3 4 5 6 range
A 1040.8
(-3)
P 		1748.1
(1.7)
P 		1348.2
(-0.9)
P 		1555.4
(0.5)
P 		1574.8
(0.6)
P 		1935.6
(3)
P 		895
B 1108.1
(-3)
P 		1590.9
(2.1)
P 		1387.4
(-0)
P 		1489.7
(1)
P 		1600.9
(2.2)
P 		1674.6
(3)
P 		567
C 1474.6
(-2.8)
P 		1516.1
(-2.2)
P 		1511.5
(-2.2)
P 		1460.9
(-3)
P 		1861.4
(3)
P 		1772.7
(1.7)
P 		401
avr 1207.8
(-3) 		1618.4
(1.2) 		1415.7
(-0.9) 		1502
(0) 		1679
(1.8) 		1794.3
(3) 		587
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 427
1 2 3 4 range
A 294.9
(-1.9)
P 		257.5
(-3)
P 		286.3
(-2.1)
P 		459.2
(3)
P 		202
B 245.9
(-3)
P 		304.5
(-2.2)
P 		280.8
(-2.5)
P 		672.5
(3)
P 		427
C 251.3
(-3)
P 		366
(-0.3)
P 		410.9
(0.7)
P 		507.6
(3)
P 		256
avr 264
(-3) 		309.3
(-2) 		326
(-1.7) 		546.4
(3) 		282

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 459
1 2 3 4 5 6 range
A 191.9
(-3)
P 		621.7
(3)
P 		293.6
(-1.6)
P 		394.5
(-0.2)
P 		475.2
(1)
P 		471.3
(0.9)
P 		430
B 385.6
(-2.1)
P 		529.6
(0.7)
P 		336.9
(-3)
P 		394.6
(-1.9)
P 		650.4
(3)
P 		624.5
(2.5)
P 		314
C 546.6
(1.9)
P 		462.6
(0.3)
P 		384.7
(-1.2)
P 		293.8
(-3)
P 		602.3
(3)
P 		593.4
(2.8)
P 		309
avr 374.7
(-2.1) 		538
(2) 		338.4
(-3) 		361
(-2.4) 		576
(3) 		563.1
(2.7) 		238
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191979_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8977
Sequence Type Exemplar sequence
Representative Public ID C09G4.2 NCBI
Target Description C09G4.2 /REP_DB=WormBase Gene ID /WP=CE03979 /TR=Q9GYS0 /GB=AAF99865.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=cAMP-dependant protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171401(11), NM_171402(11), NM_171403(11), NM_069057(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171401 NCBI Caenorhabditis elegans C09G4.2c (C09G4.2) mRNA, complete cds. 11/11 None
NM_171402 NCBI Caenorhabditis elegans C09G4.2a (C09G4.2) mRNA, complete cds. 11/11 None
NM_171403 NCBI Caenorhabditis elegans C09G4.2b (C09G4.2) mRNA, complete cds. 11/11 None
NM_069057 NCBI Caenorhabditis elegans C09G4.2d (C09G4.2) mRNA, complete cds. 11/11 None
SNAP00000025647 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8535890:8537721:1 11/11 None
GENEFINDER00000025655 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8535890:8537721:1 11/11 None
C09G4.2c ENSEMBL cdna:known chromosome:CEL140:IV:8520876:8538155:1 gene:C09G4.2 11/11 A 
C09G4.2a ENSEMBL cdna:known chromosome:CEL140:IV:8522586:8537721:1 gene:C09G4.2 11/11 None
C09G4.2b.1 ENSEMBL cdna:known chromosome:CEL140:IV:8535620:8537721:1 gene:C09G4.2 11/11 None
C09G4.2d ENSEMBL cdna:known chromosome:CEL140:IV:8535838:8537721:1 gene:C09G4.2 11/11 None
C09G4.2b.2 ENSEMBL cdna:known chromosome:CEL140:IV:8535838:8537721:1 gene:C09G4.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021734 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8523145-8537727(+) 100.0 100.0
Public Domain and Genome References
Ensembl C09G4.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM75371 Hypothetical protein C09G4.2c [Caenorhabditis elegans] ref|NP_741467.1| C09G4.2c [Caenorhabditis elegans] 0.0
blast AAM75369 Hypothetical protein C09G4.2a [Caenorhabditis elegans] ref|NP_741468.1| C09G4.2a [Caenorhabditis elegans] 0.0
blast T29830 hypothetical protein C09G4.2 - Caenorhabditis elegans 0.0
blast AAM75370 Hypothetical protein C09G4.2b [Caenorhabditis elegans] ref|NP_741469.1| C09G4.2b [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
hanks 1.1.18 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); DG2 2.0E-53
hanks 1.1.18 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); DG2 5.0E-73
hanks 1.1.18 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); DG2 3.0E-91
hanks 1.1.18 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); DG2 2.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 7.2E-80
Pfam IPR000595 EMBL-EBI Cyclic nucleotide-binding domain 3.7E-28
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-5
Pfam IPR000719 EMBL-EBI Protein kinase 7.2E-80
Pfam IPR000595 EMBL-EBI Cyclic nucleotide-binding domain 3.7E-28
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-5
Pfam IPR000719 EMBL-EBI Protein kinase 3.4E-11
Pfam IPR000719 EMBL-EBI Protein kinase 2.3E-52
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-5
Pfam IPR000719 EMBL-EBI Protein kinase 3.6E-52
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-5
Pfam IPR000719 EMBL-EBI Protein kinase 7.2E-80
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 4.4E-5
Sequence
Target Sequence 
>C. ELEGANS:191979_AT
acccgattttactgtgctggagccatggaagctcttgaatacttgcaccgaaaaaacatt
gtttatcgagatttgaagcctgaaaacatgcttctggacagaaatggatggccaaagctc
gtcgatttcggattcgccaagaaattgaagaatggcggaaggacgtggactttctgtgga
actgccgaatatgttgcaccagaaattgtactcaataagggacatgatttgtctgttgat
atttgggcacttggaattttcatgtgtgaattgttgactggatcacctccattctcaagt
accgacccaatggttacctataacgctattttgaagggactggaaaagtgggcgtggcca
agattcgtcacaaaagaagccatcgatatgatgctctccctttgtaagtatgagccaaca
gaacgtcttggctttggagatattggagaaatccgtcatcatatttggtttgacaacttt
gatttcgtcggattccgtgctcaccgaattcgtccaccattcattccatcggtggccaac
gacattgacacgtcgaactttgacactttcccagcgtttgacaacttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCCGATTTTACTGTGCTGGAGCCA 478 87 1129 Antisense
GGAAGCTCTTGAATACTTGCACCGA 383 531 1155 Antisense
AAAGCTCGTCGATTTCGGATTCGCC 228 125 1242 Antisense
GGACGTGGACTTTCTGTGGAACTGC 242 525 1289 Antisense
ATTGTTGACTGGATCACCTCCATTC 651 3 1398 Antisense
AGTGGGCGTGGCCAAGATTCGTCAC 506 59 1475 Antisense
TATGATGCTCTCCCTTTGTAAGTAT 377 655 1515 Antisense
TGATTTCGTCGGATTCCGTGCTCAC 92 563 1608 Antisense
CATTCATTCCATCGGTGGCCAACGA 157 217 1646 Antisense
GACACGTCGAACTTTGACACTTTCC 365 367 1675 Antisense
CACTTTCCCAGCGTTTGACAACTTT 58 197 1692 Antisense
Sequence Source Affymetrix Proprietary Database