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[191977_at]

Description Y87G2A.8 /REP_DB=WormBase Gene ID /WP=CE24687 /TR=Q9U1Q2 /GB=CAB60430.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=glucose-6-phosphate isomerase
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 52      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 25      6grp_total > 52      4grp_total > 61      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1287
1 2 3 4 range
A 2949.4
(3)
P 		2945.1
(3)
P 		2668.6
(1.7)
P 		1674.6
(-3)
P 		1275
B 2713.7
(2.7)
P 		2756.8
(3)
P 		2597.9
(1.9)
P 		1921.1
(-3)
P 		836
C 2961.8
(3)
P 		2626.5
(1.4)
P 		2490
(0.7)
P 		1728.4
(-3)
P 		1233
avr 2875
(3) 		2776.1
(2.5) 		2585.5
(1.4) 		1774.7
(-3) 		1100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3652
1 2 3 4 5 6 range
A 3194.7
(-1.8)
P 		4337.2
(0.5)
P 		5623.2
(3)
P 		4233.1
(0.3)
P 		3045.5
(-2.1)
P 		2585.1
(-3)
P 		3038
B 2902.6
(-2.4)
P 		3902.1
(-0.7)
P 		6088.8
(3)
P 		4314.6
(0)
P 		3205.1
(-1.9)
P 		2533.7
(-3)
P 		3555
C 3121.8
(-1.9)
P 		4202.5
(-0.2)
P 		6126.6
(3)
P 		4265.4
(-0.1)
P 		3203.3
(-1.8)
P 		2474.5
(-3)
P 		3652
avr 3073
(-2) 		4147.3
(-0.2) 		5946.2
(3) 		4271
(0.1) 		3151.3
(-1.9) 		2531.1
(-3) 		3415
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 829
1 2 3 4 range
A 1073.9
(3)
P 		639.3
(-0.1)
P 		697.6
(0.3)
P 		245.1
(-3)
P 		829
B 865.4
(3)
P 		680.9
(0.6)
P 		602
(-0.5)
P 		409.4
(-3)
P 		456
C 980.4
(3)
P 		755.1
(1)
P 		867.8
(2)
P 		291.4
(-3)
P 		689
avr 973.2
(3) 		691.8
(0.4) 		722.5
(0.7) 		315.3
(-3) 		658

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 970
1 2 3 4 5 6 range
A 588.9
(-3)
P 		1542.5
(3)
P 		1224.6
(1)
P 		1073.6
(0)
P 		919
(-0.9)
P 		629.4
(-2.7)
P 		954
B 1010
(-2.3)
P 		1298.8
(1)
P 		1478.5
(3)
P 		1142.8
(-0.8)
P 		1302.2
(1)
P 		944.8
(-3)
P 		534
C 1157.1
(-0.3)
P 		1282.3
(0.7)
P 		1559.3
(3)
P 		857.8
(-2.8)
P 		1036.5
(-1.3)
P 		828.4
(-3)
P 		731
avr 918.7
(-1.9) 		1374.5
(2.6) 		1420.8
(3) 		1024.7
(-0.8) 		1085.9
(-0.2) 		800.9
(-3) 		620
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191977_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.521
Sequence Type Exemplar sequence
Representative Public ID Y87G2A.8 NCBI
Target Description Y87G2A.8 /REP_DB=WormBase Gene ID /WP=CE24687 /TR=Q9U1Q2 /GB=CAB60430.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=glucose-6-phosphate isomerase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026667(11), NM_001026666(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026667 NCBI Caenorhabditis elegans Y87G2A.8b (Y87G2A.8) mRNA, complete cds. 11/11 None
NM_001026666 NCBI Caenorhabditis elegans Y87G2A.8a (Y87G2A.8) mRNA, complete cds. 11/11 None
SNAP00000019592 ENSEMBL cdna:SNAP chromosome:CEL140:I:13584776:13589549:1 11/11 None
GENEFINDER00000019617 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:13584776:13589549:1 11/11 None
Y87G2A.8b ENSEMBL cdna:known chromosome:CEL140:I:13584407:13589702:1 gene:Y87G2A.8 11/11 None
Y87G2A.8a.2 ENSEMBL cdna:known chromosome:CEL140:I:13584461:13589549:1 gene:Y87G2A.8 11/11 None
Y87G2A.8a.3 ENSEMBL cdna:known chromosome:CEL140:I:13584756:13589549:1 gene:Y87G2A.8 11/11 None
Y87G2A.8a.1 ENSEMBL cdna:known chromosome:CEL140:I:13584774:13589549:1 gene:Y87G2A.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:13584706-13589480(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y87G2A.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE54920 Hypothetical protein Y87G2A.8b [Caenorhabditis elegans] ref|NP_001021838.1| Y87G2A.8b [Caenorhabditis elegans] 0.0
blast CAB60430 Hypothetical protein Y87G2A.8a [Caenorhabditis elegans] ref|NP_001021837.1| Y87G2A.8a [Caenorhabditis elegans] 0.0
blast CAE63551 Hypothetical protein CBG08037 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001672 EMBL-EBI Phosphoglucose isomerase (PGI) 1.0E-126
Pfam IPR001672 EMBL-EBI Phosphoglucose isomerase (PGI) 1.0E-126
Pfam IPR001672 EMBL-EBI Phosphoglucose isomerase (PGI) 5.0E-66
Pfam IPR001672 EMBL-EBI Phosphoglucose isomerase (PGI) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191977_AT
tcgattcgccgcatatttccaacagggagatatggagagtaacgggaaatttgtgacacg
tcacggacaacgtgtcgactattcgactggaccaattgtttggggagaacctggaactaa
tggacaacacgcattttatcagttgattcatcagggtactcgactgattccagcggattt
tattgctccggttaagacgttgaatccgattcgtgggggattgcatcatcagattcttct
cgccaacttcctcgctcaaaccgaggcactaatgaaaggaaagactgccgccgtggcaga
agccgagctgaaatcatctggaatgagccctgaatcgattgcaaagattcttccacataa
agtgttcgaaggaaataaaccgacaacttctattgtacttccagttgtcacaccattcac
attgggagcattgatcgctttttatgagcacaagatctttgttcagggaattatttggga
tatttgcagttatgatcaatggggagttgagctcggaaagcaattggcaaaggttattca
accggaattggcatcagcggatacagtaacctcacatgatgcctca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGATTCGCCGCATATTTCCAACAG 671 603 1044 Antisense
TCGACTATTCGACTGGACCAATTGT 53 603 1118 Antisense
GGTACTCGACTGATTCCAGCGGATT 186 505 1198 Antisense
ATTGCTCCGGTTAAGACGTTGAATC 315 5 1225 Antisense
GATTCGTGGGGGATTGCATCATCAG 315 433 1251 Antisense
ACTTCCTCGCTCAAACCGAGGCACT 597 101 1289 Antisense
TGAAAGGAAAGACTGCCGCCGTGGC 362 571 1316 Antisense
GGAATGAGCCCTGAATCGATTGCAA 178 533 1363 Antisense
ATTGTACTTCCAGTTGTCACACCAT 504 3 1435 Antisense
GTCACACCATTCACATTGGGAGCAT 472 465 1450 Antisense
TACAGTAACCTCACATGATGCCTCA 101 645 1605 Antisense
Sequence Source Affymetrix Proprietary Database