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[191975_at] RAS-related protein

Description C35C5.4 /REP_DB=WormBase Gene ID /WP=CE18544 /GEN=mig-2 /TR=P90979 /GB=CAB01691.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 01      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 31      6grp_total > 14      4grp_total > 52      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 593
1 2 3 4 range
A 1022.9
(3)
P 		946.6
(1.8)
P 		932.2
(1.6)
P 		630.9
(-3)
P 		392
B 1034.8
(2.5)
P 		1059.8
(3)
P 		817.5
(-1.9)
P 		766
(-3)
P 		294
C 999.4
(0.6)
P 		1223.7
(3)
P 		912.8
(-0.4)
P 		668.3
(-3)
P 		555
avr 1019
(2.1) 		1076.7
(3) 		887.5
(0.1) 		688.4
(-3) 		388

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 836
1 2 3 4 5 6 range
A 1333
(3)
P 		1021.7
(0.8)
P 		940.3
(0.2)
P 		963.2
(0.3)
P 		624.6
(-2.1)
P 		496.8
(-3)
P 		836
B 1251
(3)
P 		998
(0.5)
P 		971.7
(0.3)
P 		805.3
(-1.4)
P 		664.3
(-2.8)
P 		639.1
(-3)
P 		612
C 1114.1
(3)
P 		966
(1.5)
P 		826
(0)
P 		940.9
(1.2)
P 		537.6
(-3)
P 		632.2
(-2)
P 		577
avr 1232.7
(3) 		995.2
(0.8) 		912.7
(0) 		903.1
(-0.1) 		608.8
(-2.8) 		589.4
(-3) 		643
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 280
1 2 3 4 range
A 372.5
(3)
P 		205.5
(-0.6)
P 		243.7
(0.2)
P 		92.4
(-3)
P 		280
B 330
(3)
P 		261.7
(0.5)
P 		189.4
(-2.1)
P 		163.2
(-3)
P 		167
C 330.8
(2.5)
P 		351.8
(3)
P 		318.1
(2.2)
P 		112.7
(-3)
P 		239
avr 344.4
(3) 		273
(1.1) 		250.4
(0.5) 		122.8
(-3) 		222

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 314
1 2 3 4 5 6 range
A 245.7
(0.1)
P 		363.4
(3)
P 		204.8
(-0.9)
P 		244.3
(0.1)
P 		188.5
(-1.3)
P 		121
(-3)
P 		242
B 435.3
(3)
P 		332.2
(0.2)
P 		236
(-2.4)
P 		213.3
(-3)
P 		269.9
(-1.5)
P 		238.3
(-2.3)
P 		222
C 413
(3)
P 		294.8
(0)
P 		210.2
(-2.1)
P 		189.2
(-2.6)
P 		174
(-3)
P 		211.6
(-2.1)
P 		239
avr 364.7
(3) 		330.1
(1.8) 		217
(-2.1) 		215.6
(-2.1) 		210.8
(-2.3) 		190.3
(-3) 		174
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191975_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17019
Sequence Type Exemplar sequence
Representative Public ID C35C5.4 NCBI
Target Description C35C5.4 /REP_DB=WormBase Gene ID /WP=CE18544 /GEN=mig-2 /TR=P90979 /GB=CAB01691.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=RAS-related protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077530(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077530 NCBI Caenorhabditis elegans abnormal cell MIGration family member (mig-2) (mig-2) mRNA, complete cds. 11/11 None
SNAP00000005108 ENSEMBL cdna:SNAP chromosome:CEL140:X:11548529:11550105:1 11/11 None
GENEFINDER00000005120 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11548529:11550105:1 11/11 None
C35C5.4 ENSEMBL cdna:known chromosome:CEL140:X:11548529:11550561:1 gene:C35C5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11548528-11550105(+) 100.0 100.0
Public Domain and Genome References
Gene Title RAS-related protein
Gene Symbol mig-2
Ensembl C35C5.4
Entrez Gene 181344 Entrez gene
SwissProt P90979 EMBL-EBI
WormBase CE18544 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262729_AT Rac-like GTP-binding protein (ARAC5) / Rho-like GTP-binding protein (ROP4) at
ATGENOME1:15167_S_AT Rac-like GTP-binding protein (ARAC5) / Rho-like GTP-binding protein (ROP4) at
DROSOPHILA_2:1629644_S_AT Mig-2-like dm
DROSGENOME1:150742_AT Mig-2-like dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01691 Hypothetical protein C35C5.4 [Caenorhabditis elegans] ref|NP_509931.1| abnormal cell MIGration family member (mig-2) [Caenorhabditis elegans] gb|AAC47729.1| Rac-like GTPase [Caenorhabditis elegans] 1.0E-109
blast CAE70618 Hypothetical protein CBG17302 [Caenorhabditis briggsae] 1.0E-105
blast CAB01691 Hypothetical protein C35C5.4 [Caenorhabditis elegans] ref|NP_509931.1| abnormal cell MIGration family member (mig-2) [Caenorhabditis elegans] gb|AAC47729.1| Rac-like GTPase [Caenorhabditis elegans] 1.0E-104
blast CAE70618 Hypothetical protein CBG17302 [Caenorhabditis briggsae] 1.0E-100
blast XP_624520 PREDICTED: similar to ENSANGP00000022835 [Apis mellifera] 2.0E-78
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.9E-84
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.0E-76
Sequence
Target Sequence 
>C. ELEGANS:191975_AT
tgtcttcaccgtcgaggcagatcaaatgtgtagttgttggagacggaacagttggaaaaa
catgcatgttaatatcttacacaactgactcttttccagttcagtatgtgcctacagtat
ttgataactattcggcacagatgagtcttgatgggaacgttgtgaacttaggattgtggg
atactgctggacaggaggattatgatcgtttacgaccactttcctacccacagacggatg
ttttcattctctgcttctctgtcgtctcgcccgtatcgtttgacaatgtggcaagcaagt
ggattccggaaatacgacagcattgtccagatgcgcctgtcattctagttggtaccaaac
tcgatttgcgcgacgaggccgaaccgatgcgtgctctgcaggccgaaggaaagtccccaa
tttccaaaacgcaaggcatgaaaatggctcaaaaaattaaagctgtcaagtatttggaat
gctctgcattgacgcaacagggactcacacaggtgttcgaagacgccgtacggtccattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTCTTCACCGTCGAGGCAGATCAA 117 559 14 Antisense
ATCTTACACAACTGACTCTTTTCCA 568 31 87 Antisense
ATGATCGTTTACGACCACTTTCCTA 666 23 215 Antisense
AGACGGATGTTTTCATTCTCTGCTT 469 71 245 Antisense
TCTCGCCCGTATCGTTTGACAATGT 470 611 278 Antisense
GACAGCATTGTCCAGATGCGCCTGT 85 369 329 Antisense
ATGCGCCTGTCATTCTAGTTGGTAC 445 41 344 Antisense
GGTACCAAACTCGATTTGCGCGACG 344 501 364 Antisense
TCTGCAGGCCGAAGGAAAGTCCCCA 508 597 408 Antisense
GCATTGACGCAACAGGGACTCACAC 603 307 499 Antisense
TTCGAAGACGCCGTACGGTCCATTC 599 687 529 Antisense
Sequence Source Affymetrix Proprietary Database