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[191971_at] seven trans-membrane receptor

Description K10B4.4 /REP_DB=WormBase Gene ID /WP=CE12068 /TR=O17239 /GB=AAB71009.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=seven trans-membrane receptor
Cluster 6grp_high1_10_10 > 23      4grp_high_8_8 > 10      6grp_total > 03      4grp_total > 22      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 101.4
(0.1)
P 		49.1
(-3)
P 		56.9
(-2.5)
M 		151.9
(3)
P 		103
B 120.8
(3)
P 		101.7
(0.1)
P 		86.5
(-2.3)
P 		81.7
(-3)
P 		39
C 150.2
(2)
P 		90.8
(-1.5)
P 		65.4
(-3)
P 		166.4
(3)
P 		101
avr 124.1
(2.1) 		80.5
(-2) 		69.6
(-3) 		133.3
(3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 131.4
(3)
P 		108.1
(1.5)
P 		60.3
(-1.5)
P 		98.7
(0.9)
P 		90.2
(0.4)
P 		36.4
(-3)
P 		95
B 82.2
(0.3)
P 		106
(3)
P 		75.2
(-0.4)
A 		88
(1)
P 		63.7
(-1.7)
P 		52.4
(-3)
P 		54
C 96.7
(-0.6)
P 		119.3
(3)
P 		115.8
(2.4)
P 		113.9
(2.1)
P 		81.3
(-3)
P 		92.1
(-1.3)
P 		38
avr 103.4
(2.1) 		111.1
(3) 		83.8
(-0.2) 		100.2
(1.7) 		78.4
(-0.9) 		60.3
(-3) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 36.9
(3)
P 		10.7
(-3)
P 		14.9
(-2)
M 		22.2
(-0.4)
P 		26
B 38.5
(3)
P 		25.1
(-0.8)
P 		20
(-2.3)
P 		17.4
(-3)
P 		21
C 49.7
(3)
P 		26.1
(-2.3)
P 		22.8
(-3)
P 		28.1
(-1.8)
P 		27
avr 41.7
(3) 		20.6
(-2.6) 		19.2
(-3) 		22.6
(-2.1) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 24.2
(0.1)
P 		38.4
(3)
P 		13.1
(-2.1)
P 		25
(0.3)
P 		27.2
(0.7)
P 		8.9
(-3)
P 		30
B 28.6
(0.6)
P 		35.3
(3)
P 		18.3
(-3)
A 		23.3
(-1.2)
P 		25.9
(-0.3)
P 		19.6
(-2.5)
P 		17
C 35.8
(2.7)
P 		36.4
(3)
P 		29.5
(-0.1)
P 		22.9
(-3)
P 		26.3
(-1.5)
P 		30.8
(0.5)
P 		14
avr 29.5
(0.4) 		36.7
(3) 		20.3
(-2.8) 		23.7
(-1.6) 		26.5
(-0.6) 		19.8
(-3) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191971_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4397
Sequence Type Exemplar sequence
Representative Public ID K10B4.4 NCBI
Target Description K10B4.4 /REP_DB=WormBase Gene ID /WP=CE12068 /TR=O17239 /GB=AAB71009.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=seven trans-membrane receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061265(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061265 NCBI Caenorhabditis elegans K10B4.4 (K10B4.4) mRNA, complete cds. 11/11 None
SNAP00000039348 ENSEMBL cdna:SNAP chromosome:CEL140:II:123398:125002:-1 11/11 None
GENEFINDER00000039353 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:123398:125002:-1 11/11 None
K10B4.4 ENSEMBL cdna:known chromosome:CEL140:II:123339:125002:-1 gene:K10B4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:123397-125002(-) 100.0 100.0
Public Domain and Genome References
Gene Title seven trans-membrane receptor
Ensembl K10B4.4
Entrez Gene 173398 Entrez gene
SwissProt O17239 EMBL-EBI
WormBase CE38395 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1630762_AT dm
DROSGENOME1:149874_AT dm
CHICKEN:GGAAFFX.4802.1.S1_AT similar to neuromedin U receptor 1; G protein-coupled receptor 66 gga
HG-U133_PLUS_2:221383_AT neuromedin U receptor 1 hs
HG-U133A_2:221383_AT neuromedin U receptor 1 hs
HG-U133A:221383_AT neuromedin U receptor 1 hs
HG-FOCUS:221383_AT neuromedin U receptor 1 hs
U133_X3P:G5174442_3P_AT neuromedin U receptor 1 hs
MOUSE430A_2:1421667_AT neuromedin U receptor 1 mm
MOE430A:1421667_AT neuromedin U receptor 1 mm
MOUSE430_2:1421667_AT neuromedin U receptor 1 mm
MG-U74AV2:99417_AT neuromedin U receptor 1 mm
RAE230A:1368795_AT G protein-coupled receptor 66 rn
RAT230_2:1368795_AT G protein-coupled receptor 66 rn
Protein Similarities
Method ID Description E-Value
blast NP_493666 K10B4.4 [Caenorhabditis elegans] gb|AAB71009.2| Hypothetical protein K10B4.4 [Caenorhabditis elegans] 0.0
blast CAE62805 Hypothetical protein CBG06980 [Caenorhabditis briggsae] 1.0E-180
blast CAE62805 Hypothetical protein CBG06980 [Caenorhabditis briggsae] 1.0E-178
blast CAE62805 Hypothetical protein CBG06980 [Caenorhabditis briggsae] 1.0E-170
blast XP_623966 PREDICTED: similar to putative pyrokinin receptor [Apis mellifera] 1.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.7E-69
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.5E-43
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.7E-26
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.2E-8
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.0E-22
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.7E-26
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493666.2 7 34-56,68-90,110-129,150-172,217-239,267-289,304-326
K10B4.4 7 34-56,68-90,110-129,150-172,232-254,282-304,319-341
SNAP00000039348 6 36-58,98-117,138-160,220-242,270-292,307-329
GENEFINDER00000039353 7 34-56,68-90,110-129,150-172,232-254,282-304,319-341
Sequence
Target Sequence 
>C. ELEGANS:191971_AT
gaaccaaagtcgtccggatcaacaaaaaatgattattatatttgcattcactgtgttttt
tgttattccagcgattgcgatcgttattatgtacgcgcatattgccgttcaactagaatc
atcagaaattgacttgaaaggagataaaatggtaaagaaaagaaggaacaaaagcaatag
gacagttctgaagatgttgttatcggttgtcatcacatttttcatttgctggcttccatt
ccatattcagcgacttttatctgtctatacgacgtggtccgaaaccacgacaatatcacc
tccggtgcaattcctatctatgattgtcttctacatctcaggattttgctattattcaaa
ctctgctgcgaacccaattctgtacaacattctgtctcaaaagtatcgaagtgcattttg
tcggaccattctaggggaccatattgctaattttgtgtttaaaggtcatcagagaccagg
gcagtcaaaaagatgctcttcctcaacagaagccga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCAAAGTCGTCCGGATCAACAA 518 343 678 Antisense
GTACGCGCATATTGCCGTTCAACTA 174 459 768 Antisense
TCACATTTTTCATTTGCTGGCTTCC 143 629 890 Antisense
GGCTTCCATTCCATATTCAGCGACT 12 537 908 Antisense
ATCTGTCTATACGACGTGGTCCGAA 364 33 936 Antisense
AAACCACGACAATATCACCTCCGGT 518 125 959 Antisense
CTCCGGTGCAATTCCTATCTATGAT 59 235 977 Antisense
GCTGCGAACCCAATTCTGTACAACA 168 297 1042 Antisense
CGAAGTGCATTTTGTCGGACCATTC 516 251 1084 Antisense
GGACCATTCTAGGGGACCATATTGC 530 521 1100 Antisense
GATGCTCTTCCTCAACAGAAGCCGA 418 413 1169 Antisense
Sequence Source Affymetrix Proprietary Database