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[191962_s_at] HSF-type DNA-binding domain

Description Y53C10A.12 /REP_DB=WormBase Gene ID /WP=CE22380 /TR=Q9XW45 /GB=CAA22146.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=HSF-type DNA-binding domain
Cluster 6grp_total > 48      4grp_total > 29      6grp_cgc > 18      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 134
1 2 3 4 range
A 225.2
(3)
A 		190.1
(1.4)
A 		91.1
(-3)
A 		222.7
(2.9)
A 		134
B 171.6
(3)
P 		132.7
(-2.8)
A 		131.6
(-3)
A 		133.6
(-2.7)
A 		40
C 145
(-3)
P 		191.1
(3)
A 		174.5
(0.8)
A 		170
(0.3)
A 		46
avr 180.6
(3) 		171.3
(1.8) 		132.4
(-3) 		175.4
(2.4) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 243
1 2 3 4 5 6 range
A 209.4
(-0.3)
A 		195.9
(-0.7)
P 		126
(-3)
A 		153
(-2.1)
A 		175.6
(-1.4)
A 		309
(3)
P 		183
B 175.5
(1.8)
A 		142.1
(0.3)
A 		165.5
(1.3)
A 		66.5
(-3)
A 		119.3
(-0.7)
A 		203.7
(3)
P 		137
C 266.2
(3)
P 		153.6
(-1.3)
A 		162.7
(-0.9)
A 		107.5
(-3)
A 		194.9
(0.3)
A 		211.8
(0.9)
P 		159
avr 217
(1.9) 		163.9
(-0.5) 		151.4
(-1.1) 		109
(-3) 		163.3
(-0.5) 		241.5
(3) 		133
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 58
1 2 3 4 range
A 82
(3)
A 		41.3
(-1.2)
A 		23.8
(-3)
A 		32.6
(-2.1)
A 		58
B 54.7
(3)
P 		32.8
(-2)
A 		30.5
(-2.5)
A 		28.5
(-3)
A 		26
C 48
(0.6)
P 		55
(1.9)
A 		60.8
(3)
A 		28.7
(-3)
A 		32
avr 61.6
(3) 		43
(-0.5) 		38.4
(-1.4) 		29.9
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 38.6
(-1.6)
A 		69.7
(2.3)
P 		27.4
(-3)
A 		38.8
(-1.6)
A 		53
(0.2)
A 		75.2
(3)
P 		48
B 61.1
(1.5)
A 		47.3
(0.1)
A 		40.2
(-0.7)
A 		17.6
(-3)
A 		48.5
(0.2)
A 		75.9
(3)
P 		58
C 98.7
(3)
P 		46.9
(-1)
A 		41.4
(-1.5)
A 		21.6
(-3)
A 		63.1
(0.2)
A 		70.9
(0.8)
P 		77
avr 66.1
(2) 		54.6
(0.6) 		36.3
(-1.7) 		26
(-3) 		54.9
(0.6) 		74
(3) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191962_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1224
Sequence Type Exemplar sequence
Representative Public ID Y53C10A.12 NCBI
Target Description Y53C10A.12 /REP_DB=WormBase Gene ID /WP=CE22380 /TR=Q9XW45 /GB=CAA22146.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=HSF-type DNA-binding domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060630(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060630 NCBI Caenorhabditis elegans Heat Shock Factor family member (hsf-1) (hsf-1) mRNA, complete cds. 11/11 None
GENEFINDER00000000697 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11961919:11971411:-1 11/11 None
SNAP00000000680 ENSEMBL cdna:SNAP chromosome:CEL140:I:11961925:11976782:-1 11/11 None
Y53C10A.12 ENSEMBL cdna:known chromosome:CEL140:I:11961634:11970108:-1 gene:Y53C10A.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11961849-11970015(-) 100.0 100.0
Public Domain and Genome References
Gene Title HSF-type DNA-binding domain
Gene Symbol hsf-1
Ensembl Y53C10A.12
Entrez Gene 173078 Entrez gene
SwissProt Q9XW45 EMBL-EBI
WormBase CE22380 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAS72409 heat shock transcription factor 1 [Caenorhabditis elegans] emb|CAA22146.1| Hypothetical protein Y53C10A.12 [Caenorhabditis elegans] ref|NP_493031.1| Heat Shock Factor family member (hsf-1) [Caenorhabditis elegans] 0.0
blast CAE71722 Hypothetical protein CBG18699 [Caenorhabditis briggsae] 1.0E-154
blast CAE71722 Hypothetical protein CBG18699 [Caenorhabditis briggsae] 1.0E-127
blast CAA22136 Hypothetical protein Y53C10A.2 [Caenorhabditis elegans] ref|NP_493033.1| Y53C10A.2 [Caenorhabditis elegans] 2.0E-64
blast XP_532354 PREDICTED: similar to Heat shock factor protein 1 (HSF 1) (Heat shock transcription factor 1) (HSTF 1) isoform 1 [Canis familiaris] 7.0E-43
Protein Domains
Database ID Description E-Value
scop a.2.7.Seryl-tRNA synthetase (SerRS) All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Seryl-tRNA synthetase (SerRS) 1.10000002384186
Pfam IPR000232 EMBL-EBI Heat shock factor (HSF)-type DNA-binding domain 7.8E-60
Pfam IPR000232 EMBL-EBI Heat shock factor (HSF)-type DNA-binding domain 7.0E-10
Pfam IPR000232 EMBL-EBI Heat shock factor (HSF)-type DNA-binding domain 7.8E-60
Sequence
Target Sequence 
>C. ELEGANS:191962_S_AT
ggctcaaaaacgagcggctccgtataagaatgcgactaggcaaatggctcagccgcaaca
agactattcgggaggatttgtgaacaattacagtggcttcatgccttcagatccttcaat
gattccatatcaaccatctcatcaataccttcaaccacatcagaagctaatggcaataga
agatcagcatcatccaacgacttccacctcatccaccaacgcagatcctcatcaaaatct
atattctccaactctcggactctctccatcatttgatcgtcaattatctcaagaacttca
agaatatttcactggaactgatacttcattgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTCAAAAACGAGCGGCTCCGTAT 426 533 1401 Antisense
GCGGCTCCGTATAAGAATGCGACTA 148 295 1414 Antisense
GCGACTAGGCAAATGGCTCAGCCGC 445 289 1432 Antisense
TGGCTCAGCCGCAACAAGACTATTC 367 545 1445 Antisense
TACAGTGGCTTCATGCCTTCAGATC 123 645 1489 Antisense
GCCTTCAGATCCTTCAATGATTCCA 577 281 1503 Antisense
GATTCCATATCAACCATCTCATCAA 669 433 1521 Antisense
TCATCAATACCTTCAACCACATCAG 33 621 1539 Antisense
AAGATCAGCATCATCCAACGACTTC 36 155 1580 Antisense
CACCAACGCAGATCCTCATCAAAAT 219 203 1614 Antisense
TCACTGGAACTGATACTTCATTGGA 637 621 1709 Antisense
Sequence Source Affymetrix Proprietary Database