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[191961_at]

Description E01F3.1 /REP_DB=WormBase Gene ID /WP=CE24876 /GB=CAC35868.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=3prime5prime-cyclic nucleotide phosphodiesterases
Cluster 6grp_total > 16      4grp_total > 22      6grp_cgc > 12      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 137.1
(3)
A 		8.7
(-2.9)
A 		6.8
(-3)
A 		83.2
(0.5)
A 		130
B 139.4
(2)
A 		26
(-2.3)
A 		7.5
(-3)
A 		165
(3)
A 		158
C 151.7
(2.5)
P 		39.5
(-1.9)
A 		12.7
(-3)
A 		164
(3)
A 		151
avr 142.7
(3) 		24.7
(-2.3) 		9
(-3) 		137.4
(2.8) 		134

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 96.1
(3)
A 		50.8
(0)
A 		4.1
(-3)
A 		18.2
(-2.1)
A 		25.1
(-1.6)
A 		29.6
(-1.3)
A 		92
B 115.8
(3)
A 		84
(1.3)
A 		64.1
(0.2)
A 		5.4
(-3)
A 		78.1
(1)
A 		29.4
(-1.7)
A 		110
C 164.8
(3)
A 		70.8
(-0.7)
A 		12.4
(-3)
A 		22.3
(-2.6)
A 		15.2
(-2.9)
A 		45.5
(-1.7)
A 		152
avr 125.6
(3) 		68.5
(-0.1) 		26.9
(-2.4) 		15.3
(-3) 		39.5
(-1.7) 		34.8
(-1.9) 		110
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 49.9
(3)
A 		1.9
(-3)
A 		1.8
(-3)
A 		12.2
(-1.7)
A 		48
B 44.5
(3)
A 		6.4
(-2.3)
A 		1.7
(-3)
A 		35.2
(1.7)
A 		43
C 50.2
(3)
P 		11.4
(-2.1)
A 		4.4
(-3)
A 		27.6
(0)
A 		46
avr 48.2
(3) 		6.6
(-2.5) 		2.6
(-3) 		25
(-0.1) 		46

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 60
1 2 3 4 5 6 range
A 17.7
(2.9)
A 		18.1
(3)
A 		0.9
(-3)
A 		4.6
(-1.7)
A 		7.6
(-0.7)
A 		7.2
(-0.8)
A 		17
B 40.3
(3)
A 		28
(1.1)
A 		15.6
(-0.8)
A 		1.4
(-3)
A 		31.7
(1.7)
A 		11
(-1.5)
A 		39
C 61.1
(3)
A 		21.6
(-1.1)
A 		3.1
(-3)
A 		4.5
(-2.9)
A 		4.9
(-2.8)
A 		15.2
(-1.7)
A 		58
avr 39.7
(3) 		22.6
(0.2) 		6.5
(-2.5) 		3.5
(-3) 		14.7
(-1.1) 		11.1
(-1.7) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191961_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3943
Sequence Type Exemplar sequence
Representative Public ID E01F3.1 NCBI
Target Description E01F3.1 /REP_DB=WormBase Gene ID /WP=CE24876 /GB=CAC35868.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=3prime5prime-cyclic nucleotide phosphodiesterases
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182143(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182143 NCBI Caenorhabditis elegans PhosphoDiEsterase family member (pde-3) (pde-3) mRNA, complete cds. 10/11 None
GENEFINDER00000043137 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:14951142:14952725:1 11/11 None
E01F3.1b ENSEMBL cdna:known chromosome:CEL140:II:14931128:14953233:1 gene:E01F3.1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_064683 6/11 Cross Hyb Matching Probes None
SNAP00000043032 6/11 Cross Hyb Matching Probes None
E01F3.1a 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
183981 2 1 NM_182143 191961_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:14949636-14952722(+) 100.0 100.0
Public Domain and Genome References
Ensembl E01F3.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD59173 Hypothetical protein E01F3.1b [Caenorhabditis elegans] emb|CAD59146.2| Hypothetical protein E01F3.1b [Caenorhabditis elegans] emb|CAD59150.2| Hypothetical protein E01F3.1b [Caenorhabditis elegans] ref|NP_871943.2| PhosphoDiEsterase family member (pde-3) [Caenorhabditis elegans] sp|Q8I0P7|PDE3_CAEEL Probable 3',5'-cyclic phosphodiesterase pde-3 0.0
blast CAC35868 Hypothetical protein E01F3.1a [Caenorhabditis elegans] emb|CAB07570.3| Hypothetical protein E01F3.1a [Caenorhabditis elegans] ref|NP_497084.2| PhosphoDiEsterase family member (pde-3) [Caenorhabditis elegans] 0.0
blast CAE73440 Hypothetical protein CBG20885 [Caenorhabditis briggsae] 0.0
blast CAD59173 Hypothetical protein E01F3.1b [Caenorhabditis elegans] emb|CAD59146.2| Hypothetical protein E01F3.1b [Caenorhabditis elegans] emb|CAD59150.2| Hypothetical protein E01F3.1b [Caenorhabditis elegans] ref|NP_871943.2| PhosphoDiEsterase family member (pde-3) [Caenorhabditis elegans] sp|Q8I0P7|PDE3_CAEEL Probable 3',5'-cyclic phosphodiesterase pde-3 8.0E-88
blast CAC35868 Hypothetical protein E01F3.1a [Caenorhabditis elegans] emb|CAB07570.3| Hypothetical protein E01F3.1a [Caenorhabditis elegans] ref|NP_497084.2| PhosphoDiEsterase family member (pde-3) [Caenorhabditis elegans] 8.0E-88
blast CAE73440 Hypothetical protein CBG20885 [Caenorhabditis briggsae] 1.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 2.0E-24
Pfam IPR002073 EMBL-EBI 35-cyclic nucleotide phosphodiesterase 1.4E-62
Sequence
Target Sequence 
>C. ELEGANS:191961_AT
gcttcaggactcgtttattgcacatgttgtgagcccgttggcgacagctatgaatgagtg
cggcctcctacctatcctgcccggcctagatacctcggagctcatcataaatatggagca
caaccaccgaaagtggaaagagcaaattgaacttgaaaatggtggctcatatgaggccca
gattacgtgtaatggcggaacagcagtaaacggtgtaatagaagaagaaagtgcaagtac
aagtgatagtccggaccctcgacgggattctccgttggactcggatcttagtcagtgctc
tagagtactctttttgaaaattcacaaaaaaaattataagaaaattcgaagtccatgcat
gtcaacaaaaccctattatattttcagtgatgccaagccgccagtctatctctcttttaa
aaaatcaataaatcaatattccctctggccgtctgcgtctctatctctctccctctttgc
taagctctttctcgtttcattttcctctcttgaacttccccgaatagctttcgtgcacct
ttttcttgtcattttccctcggttttatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTTCAGGACTCGTTTATTGCACAT 237 305 1089 Antisense
TGAGCCCGTTGGCGACAGCTATGAA 692 567 1118 Antisense
CTATGAATGAGTGCGGCCTCCTACC 241 179 1136 Antisense
GCCTAGATACCTCGGAGCTCATCAT 562 281 1172 Antisense
AGTACAAGTGATAGTCCGGACCCTC 61 59 1324 Antisense
TCGACGGGATTCTCCGTTGGACTCG 606 599 1347 Antisense
ACTCGGATCTTAGTCAGTGCTCTAG 527 99 1367 Antisense
CGCCAGTCTATCTCTCTTTTAAAAA 393 257 1487 Antisense
AATAAATCAATATTCCCTCTGGCCG 625 171 1515 Antisense
GAACTTCCCCGAATAGCTTTCGTGC 695 345 1600 Antisense
TTGTCATTTTCCCTCGGTTTTATCA 580 707 1634 Antisense
Sequence Source Affymetrix Proprietary Database