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[191959_at]

Description T07D1.1 /REP_DB=WormBase Gene ID /WP=CE07468 /GEN=gcy-31 /TR=Q22301 /GB=AAK71400.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster 6grp_total > 11      4grp_total > 13      6grp_cgc > 53      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 20.6
(-1.4)
A 		2.2
(-3)
A 		5.7
(-2.7)
A 		70.6
(3)
P 		68
B 31.2
(0)
A 		28.1
(-0.6)
A 		17.3
(-3)
A 		44.7
(3)
P 		27
C 52.8
(3)
A 		11.1
(-3)
A 		15.6
(-2.4)
A 		43
(1.6)
A 		42
avr 34.9
(0.3) 		13.8
(-2.9) 		12.9
(-3) 		52.8
(3) 		40

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 12
(-3)
A 		48
(2.7)
A 		49.8
(3)
P 		24
(-1.1)
A 		15.2
(-2.5)
A 		15.7
(-2.4)
A 		38
B 33.7
(2.6)
A 		36
(3)
M 		19.1
(-0)
A 		2.3
(-3)
A 		12.8
(-1.1)
A 		4.7
(-2.6)
A 		34
C 2.2
(-3)
A 		36.6
(3)
A 		5.3
(-2.5)
A 		11.6
(-1.4)
A 		11.4
(-1.4)
A 		3.7
(-2.7)
A 		34
avr 16
(-1.5) 		40.2
(3) 		24.7
(0.1) 		12.6
(-2.1) 		13.1
(-2) 		8
(-3) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 7.5
(1.3)
A 		0.5
(-3)
A 		1.5
(-2.4)
A 		10.3
(3)
P 		10
B 10
(3)
A 		6.9
(-0.1)
A 		4
(-3)
A 		9.5
(2.5)
P 		6
C 17.5
(3)
A 		3.2
(-3)
A 		5.4
(-2.1)
A 		7.2
(-1.3)
A 		14
avr 11.7
(3) 		3.5
(-3) 		3.6
(-2.9) 		9
(1) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 2.2
(-3)
A 		17.1
(3)
A 		10.8
(0.5)
P 		6.1
(-1.4)
A 		4.6
(-2)
A 		3.8
(-2.4)
A 		15
B 11.7
(2.8)
A 		12
(3)
M 		4.6
(-0.9)
A 		0.6
(-3)
A 		5.2
(-0.6)
A 		1.8
(-2.4)
A 		11
C 0.8
(-3)
A 		11.2
(3)
A 		1.3
(-2.7)
A 		2.3
(-2.1)
A 		3.7
(-1.3)
A 		1.2
(-2.8)
A 		10
avr 4.9
(-1.6) 		13.4
(3) 		5.6
(-1.2) 		3
(-2.6) 		4.5
(-1.8) 		2.3
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191959_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18364
Sequence Type Exemplar sequence
Representative Public ID T07D1.1 NCBI
Target Description T07D1.1 /REP_DB=WormBase Gene ID /WP=CE07468 /GEN=gcy-31 /TR=Q22301 /GB=AAK71400.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029719, NM_001029717, NM_001029718
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000042796 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:2420456:2427498:-1 None
T07D1.1a ENSEMBL cdna:known chromosome:CEL140:X:2420456:2427498:-1 gene:T07D1.1 None
NM_001029718 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-31) (gcy-31) mRNA, complete cds. None
T07D1.1b ENSEMBL cdna:known chromosome:CEL140:X:2420456:2427498:-1 gene:T07D1.1 None
T07D1.1c ENSEMBL cdna:known chromosome:CEL140:X:2420456:2427449:-1 gene:T07D1.1 None
NM_001029717 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-31) (gcy-31) mRNA, complete cds. None
NM_001029719 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-31) (gcy-31) mRNA, complete cds. None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042788 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2421071-2427498(-) 96.33 100.0
Public Domain and Genome References
Ensembl T07D1.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAR85901 Guanylyl cyclase protein 31, isoform b [Caenorhabditis elegans] ref|NP_001024889.1| Guanylyl CYclase family member (gcy-31) [Caenorhabditis elegans] gb|AAP32290.1| soluble guanylyl cyclase GCY-31a [Caenorhabditis elegans] sp|Q86C56|GCY31_CAEEL Soluble guanylate cyclase gcy-31 0.0
blast T16822 hypothetical protein T07D1.1 - Caenorhabditis elegans 0.0
blast CAE58877 Hypothetical protein CBG02110 [Caenorhabditis briggsae] 0.0
blast AAR85900 Guanylyl cyclase protein 31, isoform a [Caenorhabditis elegans] ref|NP_001024888.1| Guanylyl CYclase family member (gcy-31) [Caenorhabditis elegans] gb|AAP32292.1| soluble guanylyl cyclase GCY-31c [Caenorhabditis elegans] 0.0
blast AAR85902 Guanylyl cyclase protein 31, isoform c [Caenorhabditis elegans] ref|NP_001024890.1| Guanylyl CYclase family member (gcy-31) [Caenorhabditis elegans] gb|AAP32291.1| soluble guanylyl cyclase GCY-31b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 7.4E-62
Pfam IPR001054 EMBL-EBI Guanylate cyclase 7.4E-62
Pfam IPR001054 EMBL-EBI Guanylate cyclase 7.4E-62
Pfam IPR001054 EMBL-EBI Guanylate cyclase 7.4E-62
Sequence
Target Sequence 
>C. ELEGANS:191959_AT
tgtgcatcgcagtttcttgtagaagctgggaaaatggtcaatatgaataataagattcat
aaaattgatataagagccggtgtacattcaggatcggtagtagcaggagttgtcggtttg
agcatgcccagatactgtttgtttggagaaacagtttatgtcgctaataaaatggagcaa
aattcaagtccaatgaaaattttggtcagtgaaactacgcataataaaatcgaagaatca
gatccggggttgtatcagtttgagagaagagaggaaattgagataaaagacgatcagact
attcagacattttttgttgtatctcgacatggacctcaccgtgtaccttctccaagaaac
tgcgagagtcgacaagacgattcacaaaccgaagatgatgatgatgacgaacttctgtta
cccagggttttatatattttaaatattcactcggatcataagataattaaaaaaataaag
cggccgacatctcgcgaatccattatttgccttattgactttgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTGCATCGCAGTTTCTTGTAGAAG 701 555 1705 Antisense
AAGAGCCGGTGTACATTCAGGATCG 643 151 1776 Antisense
GGAGTTGTCGGTTTGAGCATGCCCA 279 517 1810 Antisense
GAGCATGCCCAGATACTGTTTGTTT 575 387 1824 Antisense
GAATCAGATCCGGGGTTGTATCAGT 28 327 1939 Antisense
TGTTGTATCTCGACATGGACCTCAC 504 563 2019 Antisense
ACCGTGTACCTTCTCCAAGAAACTG 630 87 2042 Antisense
TGATGACGAACTTCTGTTACCCAGG 358 565 2106 Antisense
ATAAAGCGGCCGACATCTCGCGAAT 646 23 2179 Antisense
ATCTCGCGAATCCATTATTTGCCTT 461 33 2193 Antisense
ATTATTTGCCTTATTGACTTTGTTC 650 13 2206 Antisense
Sequence Source Affymetrix Proprietary Database