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[191946_at] fork head DNA-binding protein

Description B0286.5 /REP_DB=WormBase Gene ID /WP=CE03865 /TR=Q10924 /GB=AAA80692.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=fork head DNA-binding protein
Cluster 6grp_total > 94      4grp_total > 50      6grp_cgc > 88      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 64.6
(-1.7)
A 		138.2
(3)
A 		112.4
(1.3)
A 		45.1
(-3)
A 		93
B 60.9
(-2)
A 		103.2
(3)
A 		85.4
(0.9)
A 		52
(-3)
A 		51
C 79.5
(1.6)
A 		85.1
(2.7)
A 		55.9
(-3)
A 		86.4
(3)
A 		31
avr 68.3
(-2.1) 		108.8
(3) 		84.6
(-0.1) 		61.2
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 91.5
(0.8)
A 		66.2
(-0.4)
A 		11.1
(-3)
A 		86.6
(0.5)
A 		139.6
(3)
P 		82.9
(0.4)
P 		129
B 62.4
(-0.5)
A 		49.7
(-1.2)
A 		61.6
(-0.5)
A 		21.3
(-3)
A 		118.4
(3)
P 		76.4
(0.4)
M 		97
C 38.8
(-2.5)
A 		31.2
(-3)
A 		63.1
(-1)
A 		124.6
(3)
P 		111.2
(2.1)
P 		86.3
(0.5)
P 		93
avr 64.2
(-1.5) 		49
(-2.7) 		45.3
(-3) 		77.5
(-0.5) 		123.1
(3) 		81.9
(-0.2) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 23.5
(1.3)
A 		30
(3)
A 		29.4
(2.8)
A 		6.6
(-3)
A 		23
B 19.4
(0.5)
A 		25.5
(3)
A 		19.8
(0.6)
A 		11.1
(-3)
A 		14
C 26.3
(3)
A 		24.5
(2.1)
A 		19.5
(-0.5)
A 		14.6
(-3)
A 		12
avr 23.1
(1.6) 		26.7
(3) 		22.9
(1.6) 		10.8
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 46
1 2 3 4 5 6 range
A 16.9
(-0.8)
A 		23.5
(0.2)
A 		2.4
(-3)
A 		22
(-0)
A 		42.1
(3)
P 		20.2
(-0.3)
P 		40
B 21.7
(-0.7)
A 		16.6
(-1.4)
A 		15
(-1.7)
A 		5.6
(-3)
A 		48.1
(3)
P 		28.5
(0.2)
M 		43
C 14.4
(-1.9)
A 		9.5
(-3)
A 		16.1
(-1.5)
A 		25.1
(0.5)
P 		36
(3)
P 		28.9
(1.4)
P 		27
avr 17.7
(-1.7) 		16.5
(-2) 		11.2
(-3) 		17.6
(-1.8) 		42.1
(3) 		25.9
(-0.1) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191946_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5959
Sequence Type Exemplar sequence
Representative Public ID B0286.5 NCBI
Target Description B0286.5 /REP_DB=WormBase Gene ID /WP=CE03865 /TR=Q10924 /GB=AAA80692.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=fork head DNA-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062374(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062374 NCBI Caenorhabditis elegans ForKHead transcription factor family member (fkh-6) (fkh-6) mRNA, complete cds. 11/11 None
SNAP00000035728 ENSEMBL cdna:SNAP chromosome:CEL140:II:4379876:4383280:-1 11/11 None
GENEFINDER00000035729 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4379876:4383280:-1 11/11 None
B0286.5 ENSEMBL cdna:known chromosome:CEL140:II:4379876:4383280:-1 gene:B0286.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4379874-4383279(-) 100.0 100.0
Public Domain and Genome References
Gene Title fork head DNA-binding protein
Gene Symbol fkh-6
Ensembl B0286.5
Entrez Gene 181907 Entrez gene
SwissProt Q10924 EMBL-EBI
WormBase CE03865 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
45449 regulation of transcription inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
30528 transcription regulator activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA80692 Forkhead transcription factor family protein 6 [Caenorhabditis elegans] ref|NP_494775.1| ForKHead transcription factor family member (fkh-6) [Caenorhabditis elegans] 1.0E-156
blast CAE60133 Hypothetical protein CBG03678 [Caenorhabditis briggsae] 1.0E-126
blast AAA80692 Forkhead transcription factor family protein 6 [Caenorhabditis elegans] ref|NP_494775.1| ForKHead transcription factor family member (fkh-6) [Caenorhabditis elegans] 1.0E-125
blast CAE60133 Hypothetical protein CBG03678 [Caenorhabditis briggsae] 3.0E-97
blast EAL29923 GA10915-PA [Drosophila pseudoobscura] 3.0E-29
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 3.00000001813429E-36
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 3.99999987306209E-20
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 4.99999996961265E-9
Pfam IPR001766 EMBL-EBI Fork head transcription factor 4.2E-47
Pfam IPR001766 EMBL-EBI Fork head transcription factor 1.9E-14
Pfam IPR001766 EMBL-EBI Fork head transcription factor 3.8E-22
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000035729 1 50-72
Sequence
Target Sequence 
>C. ELEGANS:191946_AT
aacagggaccattctacagcaacagcttccgtttttcaacgggttgaagtggccacagaa
tattcaaactatggatcctttccagttttttaagttccaataccccaactccatcactga
tagtgccaacacctcccaaatcaacaactcctcttcctcatcgtcatcatttgatacctc
aatttacacaagcgccttcccaattccaacttctcatttcgacacaaatctagtggctcc
atctcaaccaccaccagttgtgtctgatgtagaagttgtaccatcggatactgtcaaaga
ggaggttctagttgatatgaagccattgatccctgggatttcaagtacaacattcttgac
aagtcttcagatgactgatccgaggagtatcgagcaacagcaactgctctccactgcaag
caacatgtacccaaacgccttcatgcccccttataccaattggtcctgtcaaccaacaac
cactttcacgggattatcatttgatgacccatctctctatggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACAGGGACCATTCTACAGCAACAG 601 135 432 Antisense
TACAGCAACAGCTTCCGTTTTTCAA 636 641 446 Antisense
TCTTCCTCATCGTCATCATTTGATA 697 617 583 Antisense
CAAATCTAGTGGCTCCATCTCAACC 660 193 656 Antisense
AACCACCACCAGTTGTGTCTGATGT 698 147 677 Antisense
ATGAAGCCATTGATCCCTGGGATTT 45 45 748 Antisense
TCGAGCAACAGCAACTGCTCTCCAC 121 603 821 Antisense
GCTCTCCACTGCAAGCAACATGTAC 631 299 837 Antisense
CAACATGTACCCAAACGCCTTCATG 182 189 852 Antisense
CAACAACCACTTTCACGGGATTATC 230 189 905 Antisense
ATTTGATGACCCATCTCTCTATGGT 513 17 930 Antisense
Sequence Source Affymetrix Proprietary Database