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[191945_at] n-acetyllactosamine synthase

Description W02B12.11 /REP_DB=WormBase Gene ID /WP=CE25132 /TR=Q21608 /GB=CAA91401.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=n-acetyllactosamine synthase
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 39      4grp_highest_8_8 > 49      4grp_high_8_8 > 41      6grp_highest_nonorm > 73      6grp_total > 39      4grp_total > 54      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 390
1 2 3 4 range
A 664
(3)
P 		376.8
(-2.7)
P 		464.8
(-1)
P 		363.6
(-3)
P 		300
B 472.2
(3)
P 		454
(2.3)
P 		317.1
(-3)
P 		441.8
(1.8)
P 		155
C 706.7
(3)
P 		574.2
(0.9)
P 		535.1
(0.3)
P 		326.5
(-3)
P 		380
avr 614.3
(3) 		468.3
(-0.7) 		439
(-1.4) 		377.3
(-3) 		237

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 259
1 2 3 4 5 6 range
A 467.7
(-0.4)
P 		584.9
(3)
P 		498.9
(0.5)
P 		460.1
(-0.6)
P 		379.6
(-3)
P 		400.7
(-2.4)
P 		205
B 608.9
(3)
P 		481
(-2.3)
P 		464.1
(-3)
P 		558.8
(0.9)
P 		580.6
(1.8)
P 		527
(-0.4)
P 		145
C 588.5
(1.3)
P 		485.8
(-2.3)
P 		465.8
(-3)
P 		471.1
(-2.8)
P 		623
(2.5)
P 		638.7
(3)
P 		173
avr 555
(3) 		517.2
(0.1) 		476.3
(-3) 		496.7
(-1.4) 		527.7
(0.9) 		522.1
(0.5) 		79
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 189
1 2 3 4 range
A 241.8
(3)
P 		81.8
(-2.1)
P 		121.5
(-0.8)
P 		53.2
(-3)
P 		189
B 150.6
(3)
P 		112.1
(0)
P 		73.5
(-3)
P 		94.1
(-1.4)
P 		77
C 233.9
(3)
P 		165.1
(0.7)
P 		186.5
(1.4)
P 		55.1
(-3)
P 		179
avr 208.8
(3) 		119.7
(-0.8) 		127.2
(-0.5) 		67.5
(-3) 		141

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 86.2
(-3)
P 		208
(3)
P 		108.6
(-1.9)
P 		116.7
(-1.5)
P 		114.5
(-1.6)
P 		97.5
(-2.4)
P 		122
B 211.9
(1.8)
P 		160.1
(-0.7)
P 		112.7
(-3)
P 		148
(-1.3)
P 		235.9
(3)
P 		196.5
(1.1)
P 		123
C 218.1
(3)
P 		148.2
(-0.4)
P 		118.5
(-1.8)
P 		94.7
(-3)
P 		201.6
(2.2)
P 		213.8
(2.8)
P 		123
avr 172.1
(2) 		172.1
(2) 		113.3
(-3) 		119.8
(-2.4) 		184
(3) 		169.3
(1.8) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191945_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3278
Sequence Type Exemplar sequence
Representative Public ID W02B12.11 NCBI
Target Description W02B12.11 /REP_DB=WormBase Gene ID /WP=CE25132 /TR=Q21608 /GB=CAA91401.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=n-acetyllactosamine synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064048(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064048 NCBI Caenorhabditis elegans W02B12.11 (W02B12.11) mRNA, complete cds. 11/11 None
SNAP00000002916 ENSEMBL cdna:SNAP chromosome:CEL140:II:11474001:11475548:1 9/11 None
W02B12.11.1 ENSEMBL cdna:known chromosome:CEL140:II:11473766:11475925:1 gene:W02B12.11 11/11 None
W02B12.11.2 ENSEMBL cdna:known chromosome:CEL140:II:11473962:11475917:1 gene:W02B12.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000002919 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11473997-11475644(+) 100.0 100.0
Public Domain and Genome References
Gene Title n-acetyllactosamine synthase
Ensembl W02B12.11
Entrez Gene 174754 Entrez gene
SwissProt Q21608 EMBL-EBI
WormBase CE25132 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.17802.1.S1_AT similar to Beta-1,4-galactosyltransferase 5 (Beta-1,4-GalTase 5) (Beta4Gal-T5) (b4Gal-T5) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5) (Beta-1,4-GalT II)... cfa
CHICKEN:GGAAFFX.5043.1.S1_S_AT similar to Beta-1,4-galactosyltransferase 5 (Beta-1,4-GalTase 5) (Beta4Gal-T5) (b4Gal-T5) (UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5) (UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5) (Beta-1,4-GalT II)... gga
HU35KSUBA:AA248199_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HU35KSUBC:RC_N67966_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HU35KSUBC:RC_AA133969_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
U133_X3P:HS.107526.0.A3_3P_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
U133_X3P:HS.107526.0.A2_3P_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U133A_2:221484_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U133_PLUS_2:221484_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U133A:221484_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U133A_2:221485_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-FOCUS:221485_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U133_PLUS_2:221485_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U133A:221485_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U95AV2:39888_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U95B:56373_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
U133_X3P:HS2.370487.1.A1_3P_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U133_PLUS_2:1559391_S_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U95D:79520_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
HG-U95AV2:39889_AT UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 5 hs
MOE430A:1421967_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 mm
MOUSE430A_2:1421967_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 mm
MOUSE430_2:1421967_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 mm
MG-U74BV2:106071_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 mm
MOE430B:1433617_S_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 mm
MOUSE430_2:1433617_S_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 mm
MG-U74BV2:109537_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA mm
MOE430B:1444563_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA mm
MOUSE430_2:1444563_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA mm
MG-U74CV2:166953_R_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA mm
MU19KSUBA:TC15062_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA mm
MU19KSUBA:TC23195_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA mm
MU19KSUBA:TC23195_G_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), mRNA mm
RAE230A:1389898_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (predicted) rn
RAT230_2:1389898_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (predicted) rn
RG-U34B:RC_AA944170_AT UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5975 carbohydrate metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16757 transferase activity, transferring glycosyl groups inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91401 Hypothetical protein W02B12.11 [Caenorhabditis elegans] emb|CAA66831.1| N-acetyllactosamine synthase [Caenorhabditis elegans] ref|NP_496449.1| W02B12.11 [Caenorhabditis elegans] 0.0
blast CAE59496 Hypothetical protein CBG02882 [Caenorhabditis briggsae] 0.0
blast AAT11926 beta 1,4-N-acetylgalactosaminyltransferase [Trichoplusia ni] 1.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR003859 EMBL-EBI Metazoa galactosyltransferase 1.0E-126
Pfam IPR003859 EMBL-EBI Metazoa galactosyltransferase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496449.1 1 12-31
W02B12.11.1 1 12-31
W02B12.11.2 1 12-31
SNAP00000002916 1 12-31
Sequence
Target Sequence 
>C. ELEGANS:191945_AT
gattccaatacttcaacgacagcggctagattttcgattcattgttactgaacaatatgg
aaacgacttgttcaacaaaggccgaattatgaatgctgcatttatcttcgcagaaagcct
tggagtcgattgtgtagtcttccacgacgtcgacatgttcccacaagacgatcggaaccc
gtattcgtgcccacctggtccacgtcatcttggagcttttgtcagtaatctgggatatca
attgtggtacaaggagattgtcggaggcgttttagcagtatcaatggcagattatcgtgc
agttaatggatattcaaatcaattctgggcgtggggaggtgaagacgatgatatgggaca
gagaatactttcattaaattacacaattgaacgaccaaatcctgaaacagggagatattc
tatgttgaagcatgtgaagaggaaacggactgcgccgaagttgatctacaagcttctcgg
gaacagtgcaaatcgtgttgcgtacgatggattgaacgaaaccgataaatggacgattcg
aaaggtcacaacacgtccgctctactatcatctttatgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCCAATACTTCAACGACAGCGG 285 433 555 Antisense
GACAGCGGCTAGATTTTCGATTCAT 647 367 572 Antisense
CGATTGTGTAGTCTTCCACGACGTC 437 247 681 Antisense
GTCGACATGTTCCCACAAGACGATC 419 473 703 Antisense
GACGATCGGAACCCGTATTCGTGCC 214 377 721 Antisense
CACGTCATCTTGGAGCTTTTGTCAG 705 199 755 Antisense
GGAGATTGTCGGAGGCGTTTTAGCA 39 519 807 Antisense
AACGGACTGCGCCGAAGTTGATCTA 699 143 998 Antisense
AGTTGATCTACAAGCTTCTCGGGAA 579 63 1013 Antisense
TTCGAAAGGTCACAACACGTCCGCT 536 637 1091 Antisense
TCCGCTCTACTATCATCTTTATGTT 111 597 1110 Antisense
Sequence Source Affymetrix Proprietary Database