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[191931_at] Ubiquitin-conjugating enzymes

Description Y54E5B.4 /REP_DB=WormBase Gene ID /WP=CE19228 /TR=Q9XWF6 /GB=CAA21716.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Ubiquitin-conjugating enzymes
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_highest_8_8 > 63      4grp_high_8_8 > 28      6grp_highest_nonorm > 91      6grp_total > 87      4grp_total > 28      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 214.4
(3)
P 		182.4
(-0.2)
P 		157.1
(-2.7)
P 		154
(-3)
P 		60
B 204.4
(-2.8)
P 		224.2
(0.5)
P 		203.4
(-3)
P 		238.7
(3)
P 		35
C 194.5
(-2.4)
P 		220.2
(0.7)
P 		189.5
(-3)
P 		239.6
(3)
P 		50
avr 204.4
(1.6) 		208.9
(2.6) 		183.3
(-3) 		210.8
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 216
1 2 3 4 5 6 range
A 177.8
(-3)
P 		185.1
(-2.6)
P 		224.6
(-0.5)
P 		209.7
(-1.3)
P 		271.3
(2)
P 		290.4
(3)
P 		113
B 250
(0.7)
P 		136.4
(-3)
P 		232.4
(0.2)
P 		214.5
(-0.4)
P 		244.6
(0.6)
P 		318.7
(3)
P 		182
C 247.2
(0.2)
P 		131.1
(-3)
P 		217.3
(-0.6)
P 		193.1
(-1.3)
P 		219
(-0.6)
P 		347.1
(3)
P 		216
avr 225
(-0.4) 		150.9
(-3) 		224.8
(-0.4) 		205.8
(-1) 		245
(0.4) 		318.7
(3) 		168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 78
(3)
P 		39.6
(-1.2)
P 		41.1
(-1)
P 		22.5
(-3)
P 		56
B 65.2
(3)
P 		55.4
(-0.2)
P 		47.1
(-3)
P 		50.9
(-1.7)
P 		18
C 64.4
(2.6)
P 		63.3
(2.4)
P 		66
(3)
P 		40.4
(-3)
P 		26
avr 69.2
(3) 		52.8
(-0.1) 		51.4
(-0.4) 		37.9
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 32.8
(-3)
P 		65.8
(1)
P 		48.9
(-1)
P 		53.2
(-0.5)
P 		81.9
(3)
P 		70.7
(1.6)
P 		49
B 87
(0.4)
P 		45.4
(-3)
P 		56.4
(-2.1)
P 		56.8
(-2.1)
P 		99.4
(1.4)
P 		118.9
(3)
P 		74
C 91.6
(1.1)
P 		40
(-2.9)
P 		55.3
(-1.7)
P 		38.8
(-3)
P 		70.9
(-0.5)
P 		116.2
(3)
P 		77
avr 70.5
(-0.6) 		50.4
(-2.9) 		53.5
(-2.6) 		49.6
(-3) 		84.1
(1) 		101.9
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191931_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.765
Sequence Type Exemplar sequence
Representative Public ID Y54E5B.4 NCBI
Target Description Y54E5B.4 /REP_DB=WormBase Gene ID /WP=CE19228 /TR=Q9XWF6 /GB=CAA21716.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Ubiquitin-conjugating enzymes
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061186(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061186 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-16) (ubc-16) mRNA, complete cds. 11/11 None
GENEFINDER00000016376 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14830187:14831915:1 11/11 None
Y54E5B.4.1 ENSEMBL cdna:known chromosome:CEL140:I:14830166:14832417:1 gene:Y54E5B.4 11/11 None
Y54E5B.4.2 ENSEMBL cdna:known chromosome:CEL140:I:14830168:14832417:1 gene:Y54E5B.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14830117-14831782(+) 100.0 100.0
Public Domain and Genome References
Gene Title Ubiquitin-conjugating enzymes
Gene Symbol ubc-16
Ensembl Y54E5B.4
Entrez Gene 173354 Entrez gene
SwissProt Q9XWF6 EMBL-EBI
WormBase CE19228 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261110_AT ubiquitin-conjugating enzyme 16 (UBC16) at
ATGENOME1:18599_AT ubiquitin-conjugating enzyme 16 (UBC16) at
CANINE_2:CFAAFFX.12945.1.S1_AT similar to CG7220-PA, isoform A cfa
DROSOPHILA_2:1637771_S_AT dm
DROSGENOME1:153071_AT dm
HG-U133_PLUS_2:218521_S_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133A:218521_S_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133A_2:218521_S_AT ubiquitin-conjugating enzyme E2W (putative) hs
HU35KSUBA:RC_D25755_S_AT ubiquitin-conjugating enzyme E2W (putative) hs
HU35KSUBA:W92515_AT ubiquitin-conjugating enzyme E2W (putative) hs
HU35KSUBB:RC_N73275_AT ubiquitin-conjugating enzyme E2W (putative) hs
HU35KSUBB:RC_AA226963_AT ubiquitin-conjugating enzyme E2W (putative) hs
HU35KSUBD:RC_AA453418_AT ubiquitin-conjugating enzyme E2W (putative) hs
U133_X3P:G8922821_3P_A_AT ubiquitin-conjugating enzyme E2W (putative) hs
U133_X3P:HS2.376816.1.S1_3P_AT ubiquitin-conjugating enzyme E2W (putative) hs
U133_X3P:HS2.21275.2.S1_3P_AT ubiquitin-conjugating enzyme E2W (putative) hs
U133_X3P:HS.21275.0.A3_3P_AT ubiquitin-conjugating enzyme E2W (putative) hs
U133_X3P:HS.21275.0.A1_3P_A_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133_PLUS_2:1555217_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133_PLUS_2:1562458_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133_PLUS_2:222656_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133B:222656_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133_PLUS_2:222657_S_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U133B:222657_S_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U95B:46903_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U95B:59735_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U95C:60199_AT ubiquitin-conjugating enzyme E2W (putative) hs
HG-U95C:60791_AT ubiquitin-conjugating enzyme E2W (putative) hs
HU35KSUBC:RC_N66498_AT Ubiquitin-conjugating enzyme E2W (putative) hs
MG-U74AV2:103582_R_AT ubiquitin-conjugating enzyme E2W (putative) mm
MG-U74CV2:134526_R_AT ubiquitin-conjugating enzyme E2W (putative) mm
MG-U74CV2:134525_F_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOE430A:1452054_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOUSE430A_2:1452054_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOUSE430_2:1452054_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOE430A:1426398_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOUSE430A_2:1426398_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOUSE430_2:1426398_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOE430B:1457544_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOUSE430_2:1457544_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOE430B:1443188_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOUSE430_2:1443188_AT ubiquitin-conjugating enzyme E2W (putative) mm
MOE430B:1444917_AT RIKEN cDNA 6130401J04 gene, mRNA (cDNA clone MGC:28304 IMAGE:4012785) mm
MOUSE430_2:1444917_AT RIKEN cDNA 6130401J04 gene, mRNA (cDNA clone MGC:28304 IMAGE:4012785) mm
MU19KSUBC:TC35519_AT RIKEN cDNA 6130401J04 gene, mRNA (cDNA clone MGC:28304 IMAGE:4012785) mm
MU19KSUBC:TC41287_AT RIKEN cDNA 6130401J04 gene, mRNA (cDNA clone MGC:28304 IMAGE:4012785) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21716 Hypothetical protein Y54E5B.4 [Caenorhabditis elegans] ref|NP_493587.1| UBiquitin Conjugating enzyme family member (ubc-16) [Caenorhabditis elegans] 2.0E-85
blast CAE72523 Hypothetical protein CBG19703 [Caenorhabditis briggsae] 3.0E-77
blast CAA21716 Hypothetical protein Y54E5B.4 [Caenorhabditis elegans] ref|NP_493587.1| UBiquitin Conjugating enzyme family member (ubc-16) [Caenorhabditis elegans] 3.0E-71
blast CAE72523 Hypothetical protein CBG19703 [Caenorhabditis briggsae] 8.0E-66
blast XP_392626 PREDICTED: similar to ENSANGP00000024002 [Apis mellifera] 1.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 4.3E-46
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 2.8E-44
Sequence
Target Sequence 
>C. ELEGANS:191931_AT
gatgctgcgacaagacgactgatgaaagagctggctcaactgaaaagtgaggctccagaa
gggcttttagtggataatacatcaacaagtaacgatttgaaacaatggaaaataggagtc
gtcggcgccgagggaacattgtatgcgggtgaagtttttatgctgcaattcacgtttggt
cctcagtacccgttcaattctccggaggtgatgttcgtcggcgaaactatcccagcccac
ccgcacatctactcaaatggacatatctgcctgtctatcctatcagatgattggacacct
gctctcagtgttcaatccgtgtgcctctcgattctgtcaatgctttcatcgtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGCTGCGACAAGACGACTGATGA 536 413 19 Antisense
GAAAATAGGAGTCGTCGGCGCCGAG 473 351 126 Antisense
GTCGGCGCCGAGGGAACATTGTATG 578 471 139 Antisense
GCGGGTGAAGTTTTTATGCTGCAAT 498 291 163 Antisense
CAATTCACGTTTGGTCCTCAGTACC 583 179 184 Antisense
AATTCTCCGGAGGTGATGTTCGTCG 42 173 214 Antisense
TGTTCGTCGGCGAAACTATCCCAGC 124 561 230 Antisense
CACCCGCACATCTACTCAAATGGAC 420 201 256 Antisense
TGGACATATCTGCCTGTCTATCCTA 178 549 276 Antisense
TCAGATGATTGGACACCTGCTCTCA 22 623 301 Antisense
GATTCTGTCAATGCTTTCATCGTCA 128 433 348 Antisense
Sequence Source Affymetrix Proprietary Database