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[191924_s_at] 2-oxoisovalerate dehydrogenase

Description F27D4.5 /REP_DB=WormBase Gene ID /WP=CE05736 /TR=Q93619 /GB=CAB01970.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=2-oxoisovalerate dehydrogenase
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 31      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 33      6grp_total > 42      4grp_total > 60      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2767
1 2 3 4 range
A 2521.2
(1.4)
P 		2063.8
(0.3)
P 		3169.9
(3)
P 		688.5
(-3)
P 		2481
B 2497.4
(1.1)
P 		2577.5
(1.3)
P 		3380
(3)
P 		613.4
(-3)
P 		2767
C 2295.3
(1.5)
P 		2733.9
(2.7)
P 		2860.5
(3)
P 		622.9
(-3)
P 		2238
avr 2438
(1.3) 		2458.4
(1.4) 		3136.8
(3) 		641.6
(-3) 		2495

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1551
1 2 3 4 5 6 range
A 1990.3
(1.1)
P 		2226.6
(2.4)
P 		2332.6
(3)
P 		1803.6
(0)
P 		1419.5
(-2.1)
P 		1257.8
(-3)
P 		1075
B 2024.9
(0.9)
P 		1860
(0.1)
P 		2491.5
(3)
P 		1820.9
(-0)
P 		1412.1
(-1.9)
P 		1162.3
(-3)
P 		1329
C 1872.2
(-0.5)
P 		1893.2
(-0.4)
P 		2712.9
(3)
P 		1763.6
(-1)
P 		1400.8
(-2.5)
P 		1273
(-3)
P 		1440
avr 1962.5
(0.4) 		1993.3
(0.6) 		2512.3
(3) 		1796
(-0.4) 		1410.8
(-2.2) 		1231
(-3) 		1281
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 896
1 2 3 4 range
A 918
(3)
P 		448
(-0.5)
P 		828.7
(2.3)
P 		100.8
(-3)
P 		817
B 796.4
(3)
P 		636.6
(1.6)
P 		783.2
(2.9)
P 		130.7
(-3)
P 		666
C 759.8
(1.4)
P 		786
(1.6)
P 		997
(3)
P 		105
(-3)
P 		892
avr 824.7
(2.6) 		623.5
(1.1) 		869.6
(3) 		112.2
(-3) 		757

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 486
1 2 3 4 5 6 range
A 366.9
(-2.3)
P 		791.9
(3)
P 		508
(-0.5)
P 		457.4
(-1.1)
P 		428.3
(-1.5)
P 		306.2
(-3)
P 		486
B 704.6
(3)
P 		619.1
(1.1)
P 		605
(0.8)
P 		482.3
(-1.9)
P 		573.7
(0.1)
P 		433.4
(-3)
P 		271
C 693.9
(3)
P 		577.7
(0.9)
P 		690.5
(2.9)
P 		354.7
(-3)
P 		453.3
(-1.3)
P 		426.2
(-1.7)
P 		339
avr 588.5
(1.4) 		662.9
(3) 		601.2
(1.7) 		431.5
(-2.1) 		485.1
(-0.9) 		388.6
(-3) 		274
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191924_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1515
Sequence Type Exemplar sequence
Representative Public ID F27D4.5 NCBI
Target Description F27D4.5 /REP_DB=WormBase Gene ID /WP=CE05736 /TR=Q93619 /GB=CAB01970.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=2-oxoisovalerate dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059748(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059748 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-173) (tag-173) mRNA, complete cds. 11/11 None
SNAP00000008677 ENSEMBL cdna:SNAP chromosome:CEL140:I:7735394:7736938:1 11/11 None
GENEFINDER00000008685 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:7735394:7745987:1 11/11 None
F27D4.5.1 ENSEMBL cdna:known chromosome:CEL140:I:7735392:7737203:1 gene:F27D4.5 11/11 None
F27D4.5.2 ENSEMBL cdna:known chromosome:CEL140:I:7735394:7737203:1 gene:F27D4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7735324-7736869(+) 100.0 100.0
Public Domain and Genome References
Gene Title 2-oxoisovalerate dehydrogenase
Gene Symbol tag-173
Ensembl F27D4.5
Entrez Gene 172539 Entrez gene
SwissProt Q93619 EMBL-EBI
WormBase CE05736 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:265070_AT 2-oxoisovalerate dehydrogenase, putative / 3-methyl-2-oxobutanoate dehydrogenase, putative / branched-chain alpha-keto acid dehydrogenase E1 beta subunit, putative at
ATGENOME1:15670_S_AT 2-oxoisovalerate dehydrogenase, putative / 3-methyl-2-oxobutanoate dehydrogenase, putative / branched-chain alpha-keto acid dehydrogenase E1 beta subunit, putative at
CANINE:1586610_AT similar to 2-oxoisovalerate dehydrogenase beta subunit, mitochondrial precursor (Branched-chain alpha-keto acid dehydrogenase E1 component beta chain) (BCKDH E1-beta) cfa
CANINE:1584611_AT similar to 2-oxoisovalerate dehydrogenase beta subunit, mitochondrial precursor (Branched-chain alpha-keto acid dehydrogenase E1 component beta chain) (BCKDH E1-beta) cfa
CANINE_2:CFA.11286.1.A1_AT similar to 2-oxoisovalerate dehydrogenase beta subunit, mitochondrial precursor (Branched-chain alpha-keto acid dehydrogenase E1 component beta chain) (BCKDH E1-beta) cfa
CANINE_2:CFA.18456.1.S1_S_AT similar to 2-oxoisovalerate dehydrogenase beta subunit, mitochondrial precursor (Branched-chain alpha-keto acid dehydrogenase E1 component beta chain) (BCKDH E1-beta) cfa
CHICKEN:GGA.3355.1.S1_S_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) gga
HUGENEFL:U50708_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U133A:210653_S_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U133_PLUS_2:210653_S_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-FOCUS:210653_S_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U133A_2:210653_S_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
U133_X3P:G179361_3P_A_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
U133_X3P:HS.1265.2.A1_3P_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U133A_2:213321_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U133_PLUS_2:213321_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U133A:213321_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U95AV2:41682_S_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U95AV2:41683_I_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U95AV2:41684_AT branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U95C:60364_R_AT Branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HG-U95D:77414_I_AT Branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
HU35KSUBC:RC_T59946_AT Branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) hs
MG-U74AV2:102302_AT branched chain ketoacid dehydrogenase E1, beta polypeptide mm
MOE430A:1427153_AT branched chain ketoacid dehydrogenase E1, beta polypeptide mm
MOUSE430A_2:1427153_AT branched chain ketoacid dehydrogenase E1, beta polypeptide mm
MOUSE430_2:1427153_AT branched chain ketoacid dehydrogenase E1, beta polypeptide mm
MU19KSUBB:TC32179_AT Branched chain ketoacid dehydrogenase E1, beta polypeptide (Bckdhb), mRNA mm
RG-U34A:RC_AI168942_AT branched chain keto acid dehydrogenase E1, beta polypeptide rn
RAE230A:1370906_AT branched chain keto acid dehydrogenase E1, beta polypeptide rn
RAT230_2:1370906_AT branched chain keto acid dehydrogenase E1, beta polypeptide rn
Protein Similarities
Method ID Description E-Value
blast CAB01970 Hypothetical protein F27D4.5 [Caenorhabditis elegans] ref|NP_492149.1| Temporarily Assigned Gene name family member (tag-173) [Caenorhabditis elegans] 0.0
blast CAB01971 Hypothetical protein F27D4.6 [Caenorhabditis elegans] ref|NP_492150.2| F27D4.6 [Caenorhabditis elegans] 0.0
blast XP_343440 PREDICTED: branched chain keto acid dehydrogenase E1, beta polypeptide [Rattus norvegicus] 1.0E-121
blast NP_898871 branched chain keto acid dehydrogenase E1, beta polypeptide precursor [Homo sapiens] ref|NP_000047.1| branched chain keto acid dehydrogenase E1, beta polypeptide precursor [Homo sapiens] gb|AAV38866.1| branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) [Homo sapiens] emb|CAI15049.1| branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) [Homo sapiens] emb|CAC36881.2| branched chain keto acid dehydrogenase E1, beta polypeptide (maple syrup urine disease) [Homo sapiens] gb|AAH40139.1| Branched chain keto acid dehydrogenase E1, beta polypeptide, precursor [Homo sapiens] dbj|BAA14389.1| E-1-beta subunit of branched chain alpha-keto acid dehydrogenase [Homo sapiens] gb|AAX41176.1| branched chain keto acid dehydrogenase E1 beta polypeptide [synthetic construct] sp|P21953|ODBB_HUMAN 2-oxoisovalerate dehydrogenase beta subunit, mitochondrial precursor (Branched-chain alpha-keto acid dehydrogenase E1 component beta chain) (BCKDH E1-beta) gb|AAB16763.1| branched chain alpha-ketoacid dehydrogenase E1 beta subunit gb|AAA51812.1| branched chain alpha-keto acid dehydrogenase E1-beta subunit 1.0E-121
Protein Domains
Database ID Description E-Value
Pfam IPR005476 EMBL-EBI Transketolase, C terminal 9.7E-54
Pfam IPR005475 EMBL-EBI Transketolase, central region 7.4E-71
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000008685 2 373-395,415-437
Sequence
Target Sequence 
>C. ELEGANS:191924_S_AT
gagtcctgaagctaacttcacccataccccaggattaaagcttgttgttccacgtgggcc
agttcaagccaaaggacttcttctgtcgtgcattcgtgatccgaatccatgtattttctt
cgaaccaaagattctttatcgattggcttctgaagatgttccaactggagattacacaat
tccactgggacaagctgaaactgttcgttctggaaaagatttgacacttgttgcctgggg
aacacaggttcatgttgctcttgaagcagctcaattggccaaggagaagctcaatgcaga
tgttgaagttattgatcttcagacgattcaaccttgggacgaggatcatgtagtagagag
tgttcaaaaaactggtcgactcattgtcactcatgaagctccgatttcttctggttttgg
agctgaaattgcttctactgttcagaaacgttgcttcctgaaccttgaatctccaataga
tcgtgttgctggattcgatacaccattcccacacgttcacgaaccattctacctgccaac
tgttcatcgagtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTCCTGAAGCTAACTTCACCCAT 491 399 528 Antisense
CTTCACCCATACCCCAGGATTAAAG 401 217 543 Antisense
TGTTCCACGTGGGCCAGTTCAAGCC 443 561 573 Antisense
GCCAAAGGACTTCTTCTGTCGTGCA 575 277 595 Antisense
TTCTGTCGTGCATTCGTGATCCGAA 321 695 608 Antisense
GATTTGACACTTGTTGCCTGGGGAA 585 425 745 Antisense
ACTGGTCGACTCATTGTCACTCATG 276 97 898 Antisense
GTCACTCATGAAGCTCCGATTTCTT 425 465 913 Antisense
ACGTTGCTTCCTGAACCTTGAATCT 131 93 975 Antisense
ACACGTTCACGAACCATTCTACCTG 597 109 1038 Antisense
CTACCTGCCAACTGTTCATCGAGTT 182 225 1056 Antisense
Sequence Source Affymetrix Proprietary Database