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[191914_at]

Description T20H4.3 /REP_DB=WormBase Gene ID /WP=CE00748 /TR=Q22620 /GB=AAA50660.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Prolyl-tRNA synthetase region
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 21      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 23      6grp_total > 31      4grp_total > 60      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2428
1 2 3 4 range
A 2968.5
(2.1)
P 		3298.2
(3)
P 		2998.3
(2.2)
P 		1069.6
(-3)
P 		2229
B 2880.9
(1.4)
P 		2932.3
(1.5)
P 		3497.9
(3)
P 		1251.9
(-3)
P 		2246
C 2845.5
(1.8)
P 		2536.5
(1)
P 		3252.6
(3)
P 		1144.8
(-3)
P 		2108
avr 2898.3
(2) 		2922.3
(2.1) 		3249.6
(3) 		1155.4
(-3) 		2094

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1368
1 2 3 4 5 6 range
A 2544.3
(3)
P 		2531.1
(2.9)
P 		2212.5
(0.2)
P 		2496.9
(2.6)
P 		1836.8
(-3)
P 		1925.8
(-2.2)
P 		708
B 2223.1
(-0.5)
P 		2537.6
(0.9)
P 		3016.7
(3)
P 		2510.8
(0.8)
P 		1961.5
(-1.6)
P 		1648.7
(-3)
P 		1368
C 2478.2
(1.8)
P 		2656.8
(3)
P 		2603.2
(2.7)
P 		2221.7
(0.2)
P 		1798.5
(-2.6)
P 		1733.6
(-3)
P 		923
avr 2415.2
(1.6) 		2575.2
(2.7) 		2610.8
(3) 		2409.8
(1.6) 		1865.6
(-2.3) 		1769.4
(-3) 		841
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 977
1 2 3 4 range
A 1080.8
(3)
P 		715.9
(0.6)
P 		783.8
(1.1)
P 		156.6
(-3)
P 		924
B 918.7
(3)
P 		724.2
(1.2)
P 		810.5
(2)
P 		266.8
(-3)
P 		652
C 941.9
(1.8)
P 		729.3
(0.4)
P 		1133.6
(3)
P 		193
(-3)
P 		941
avr 980.5
(3) 		723.1
(1) 		909.3
(2.4) 		205.5
(-3) 		775

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 472
1 2 3 4 5 6 range
A 469
(-3)
P 		900.2
(3)
P 		481.8
(-2.8)
P 		633.3
(-0.7)
P 		554.3
(-1.8)
P 		468.9
(-3)
P 		431
B 773.5
(1.1)
P 		844.7
(3)
P 		732.6
(0.1)
P 		665
(-1.7)
P 		796.9
(1.8)
P 		614.8
(-3)
P 		230
C 918.5
(3)
P 		810.7
(1.6)
P 		662.5
(-0.3)
P 		446.8
(-3)
P 		582
(-1.3)
P 		580.3
(-1.3)
P 		472
avr 720.3
(0.3) 		851.9
(3) 		625.6
(-1.6) 		581.7
(-2.5) 		644.4
(-1.2) 		554.7
(-3) 		297
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191914_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7427
Sequence Type Exemplar sequence
Representative Public ID T20H4.3 NCBI
Target Description T20H4.3 /REP_DB=WormBase Gene ID /WP=CE00748 /TR=Q22620 /GB=AAA50660.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Prolyl-tRNA synthetase region
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027606(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027606 NCBI Caenorhabditis elegans Prolyl tRNA Synthetase family member (prs-1) (prs-1) mRNA, complete cds. 11/11 None
SNAP00000008520 ENSEMBL cdna:SNAP chromosome:CEL140:III:7238962:7241025:1 11/11 None
GENEFINDER00000008525 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7238962:7242824:1 11/11 None
T20H4.3a.2 ENSEMBL cdna:known chromosome:CEL140:III:7238946:7241193:1 gene:T20H4.3 11/11 None
T20H4.3a.1 ENSEMBL cdna:known chromosome:CEL140:III:7238951:7241208:1 gene:T20H4.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001027607 8/11 Cross Hyb Matching Probes None
T20H4.3b 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176024 2 1 NM_001027606 191914_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7238959-7241023(+) 100.0 100.0
Public Domain and Genome References
Ensembl T20H4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA50660 Prolyl trna synthetase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001022777.1| Prolyl tRNA Synthetase family member (prs-1) [Caenorhabditis elegans] 0.0
blast CAE56448 Hypothetical protein CBG24153 [Caenorhabditis briggsae] 0.0
blast AAU20840 Prolyl trna synthetase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001022778.1| Prolyl tRNA Synthetase family member (prs-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002314 EMBL-EBI tRNA synthetases, class-II (G, H, P and S) 1.2E-26
Pfam IPR004154 EMBL-EBI Anticodon binding domain 7.7E-30
Pfam IPR000738 EMBL-EBI WHEP-TRS 5.7E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000008525 2 585-607,627-649
Sequence
Target Sequence 
>C. ELEGANS:191914_AT
aagttattgttgttccagttggattcaagactgagaacaagcaatcgcttttcgatgctg
ttgataaggttaccaaggatttggtcgcggatggagtaaaggctgagcacgatttgagag
aaaactacaatgctggctggaagttcaatcattgggaattgaaaggagtcccaatccgtt
ttgaggtcggaccaaatgatttggcaaagaaccaggtaaccgctgtaatccgtcacaacg
gagagaagaaacaactttccctggaaggactgtctaaaactgtcaaatctcttcttgatg
aaattcatactgctatgtacaataaggtactcgctcaacgtgactctcacctgaagagca
ccacgtgcatcgaagaattcaagaagcttcttgacgagaagtgcattatactgtccccat
tctgtggtcgtccagactgtgaagaggaaatcaagaaggcatcaacaagagaagacggag
aaggcgcacagatgggagccaagacgttatgtattccattggatcaaccaaaacaagaac
ttccatcaaaatgtcttttcccatcatgcacatacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTTATTGTTGTTCCAGTTGGATT 667 155 1145 Antisense
GGAGTCCCAATCCGTTTTGAGGTCG 428 505 1309 Antisense
CCAGGTAACCGCTGTAATCCGTCAC 461 261 1356 Antisense
ACAACTTTCCCTGGAAGGACTGTCT 102 111 1395 Antisense
ATAAGGTACTCGCTCAACGTGACTC 215 25 1466 Antisense
GTGACTCTCACCTGAAGAGCACCAC 337 483 1484 Antisense
AGAAGTGCATTATACTGTCCCCATT 467 75 1541 Antisense
CCATTCTGTGGTCGTCCAGACTGTG 23 271 1561 Antisense
GAGCCAAGACGTTATGTATTCCATT 335 385 1640 Antisense
ACTTCCATCAAAATGTCTTTTCCCA 195 103 1683 Antisense
GTCTTTTCCCATCATGCACATACAA 312 469 1697 Antisense
Sequence Source Affymetrix Proprietary Database