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[191912_at]

Description C13B9.4 /REP_DB=WormBase Gene ID /WP=CE01779 /TR=SW:Q09460 /GB=AAA62517.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_highest_8_8 > 52      4grp_high_8_8 > 17      6grp_highest_nonorm > 30      6grp_total > 26      4grp_total > 31      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Cup      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 863
1 2 3 4 range
A 1238.5
(3)
P 		399.7
(-3)
P 		450.2
(-2.6)
P 		771.4
(-0.3)
P 		839
B 1233.7
(3)
P 		521.9
(-2)
P 		375.4
(-3)
P 		842.8
(0.3)
P 		858
C 1199.8
(3)
P 		527.6
(-2.7)
P 		489.6
(-3)
P 		858.6
(0.1)
P 		710
avr 1224
(3) 		483.1
(-2.7) 		438.4
(-3) 		824.3
(-0.1) 		786

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 730
1 2 3 4 5 6 range
A 1018.8
(3)
P 		938.3
(1.9)
P 		584.5
(-3)
P 		637
(-2.3)
P 		594.7
(-2.9)
P 		583.9
(-3)
P 		435
B 1194.8
(3)
P 		961.7
(0.8)
P 		686.5
(-1.9)
P 		640.1
(-2.3)
P 		587
(-2.8)
P 		566.7
(-3)
P 		628
C 1005.2
(2.2)
P 		1085.2
(3)
P 		690.6
(-0.8)
P 		683.6
(-0.9)
P 		464.5
(-3)
P 		581.3
(-1.9)
P 		621
avr 1072.9
(3) 		995.1
(2.1) 		653.9
(-1.8) 		653.6
(-1.8) 		548.7
(-3) 		577.3
(-2.7) 		524
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 364
1 2 3 4 range
A 451
(3)
P 		86.8
(-3)
P 		117.7
(-2.5)
P 		112.9
(-2.6)
P 		364
B 393.4
(3)
P 		128.9
(-2.2)
P 		87
(-3)
P 		179.6
(-1.2)
P 		306
C 397.2
(3)
P 		151.7
(-2.8)
P 		170.6
(-2.4)
P 		144.8
(-3)
P 		252
avr 413.9
(3) 		122.5
(-3) 		125.1
(-2.9) 		145.8
(-2.5) 		291

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 288
1 2 3 4 5 6 range
A 187.8
(-1.2)
P 		333.7
(3)
P 		127.3
(-3)
P 		161.6
(-2)
P 		179.5
(-1.5)
P 		142.2
(-2.6)
P 		206
B 415.7
(3)
P 		320.1
(0.7)
P 		166.7
(-3)
P 		169.5
(-2.9)
P 		238.5
(-1.3)
P 		211.3
(-1.9)
P 		249
C 372.6
(3)
P 		331.1
(1.9)
P 		175.8
(-2)
P 		137.5
(-3)
P 		150.3
(-2.7)
P 		194.6
(-1.5)
P 		235
avr 325.4
(2.9) 		328.3
(3) 		156.6
(-3) 		156.2
(-3) 		189.4
(-1.8) 		182.7
(-2.1) 		172
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191912_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6314
Sequence Type Exemplar sequence
Representative Public ID C13B9.4 NCBI
Target Description C13B9.4 /REP_DB=WormBase Gene ID /WP=CE01779 /TR=SW:Q09460 /GB=AAA62517.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026001(11), NM_001025999(11), NM_001026000(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026001 NCBI Caenorhabditis elegans C13B9.4c (C13B9.4) mRNA, complete cds. 11/11 None
NM_001025999 NCBI Caenorhabditis elegans C13B9.4a (C13B9.4) mRNA, complete cds. 11/11 None
NM_001026000 NCBI Caenorhabditis elegans C13B9.4b (C13B9.4) mRNA, complete cds. 11/11 None
GENEFINDER00000006909 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6633374:6638759:-1 11/11 None
SNAP00000006904 ENSEMBL cdna:SNAP chromosome:CEL140:III:6634017:6636432:-1 11/11 None
C13B9.4b.2 ENSEMBL cdna:known chromosome:CEL140:III:6632776:6652018:-1 gene:C13B9.4 11/11 A 
C13B9.4c.2 ENSEMBL cdna:known chromosome:CEL140:III:6632776:6652018:-1 gene:C13B9.4 11/11 A 
C13B9.4a ENSEMBL cdna:known chromosome:CEL140:III:6632776:6652018:-1 gene:C13B9.4 11/11 A 
C13B9.4b.1 ENSEMBL cdna:known chromosome:CEL140:III:6633374:6638781:-1 gene:C13B9.4 11/11 None
C13B9.4c.1 ENSEMBL cdna:known chromosome:CEL140:III:6634017:6650960:-1 gene:C13B9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6634015-6638758(-) 100.0 100.0
Public Domain and Genome References
Ensembl C13B9.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAT92086 Hypothetical protein C13B9.4c [Caenorhabditis elegans] ref|NP_001021172.1| C13B9.4c [Caenorhabditis elegans] gb|AAQ84884.1| calcitonin receptor-like protein SEB-1B [Caenorhabditis elegans] 0.0
blast AAN73883 Hypothetical protein C13B9.4a [Caenorhabditis elegans] ref|NP_001021170.1| C13B9.4a [Caenorhabditis elegans] gb|AAQ84885.1| calcitonin receptor-like protein SEB-1C [Caenorhabditis elegans] sp|Q09460|SEB1_CAEEL Calcitonin receptor-like protein seb-1 0.0
blast AAT92085 Hypothetical protein C13B9.4b [Caenorhabditis elegans] ref|NP_001021171.1| C13B9.4b [Caenorhabditis elegans] gb|AAQ84883.1| calcitonin receptor-like protein SEB-1A [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001879 EMBL-EBI Hormone receptor, extracellular 1.1E-19
Pfam IPR000832 EMBL-EBI G-protein coupled receptor family 2 (secretin-like) 8.0E-90
Pfam IPR001879 EMBL-EBI Hormone receptor, extracellular 1.2E-19
Pfam IPR000832 EMBL-EBI G-protein coupled receptor family 2 (secretin-like) 8.0E-90
Pfam IPR001879 EMBL-EBI Hormone receptor, extracellular 5.2E-5
Pfam IPR000832 EMBL-EBI G-protein coupled receptor family 2 (secretin-like) 8.0E-90
Pfam IPR000832 EMBL-EBI G-protein coupled receptor family 2 (secretin-like) 8.0E-90
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021172.1 6 170-192,202-221,252-274,289-311,375-397,407-429
NP_001021170.1 6 170-192,202-221,252-274,289-311,375-397,407-429
NP_001021171.1 6 160-182,192-211,242-264,279-301,365-387,397-419
C13B9.4b.2 6 160-182,192-211,242-264,279-301,365-387,397-419
C13B9.4c.2 6 170-192,202-221,252-274,289-311,375-397,407-429
C13B9.4a 6 170-192,202-221,252-274,289-311,375-397,407-429
C13B9.4b.1 6 160-182,192-211,242-264,279-301,365-387,397-419
C13B9.4c.1 6 170-192,202-221,252-274,289-311,375-397,407-429
GENEFINDER00000006909 7 273-295,307-329,364-386,393-415,435-457,478-500,510-532
SNAP00000006904 7 44-66,78-100,135-157,164-186,206-228,249-271,281-303
Sequence
Target Sequence 
>C. ELEGANS:191912_AT
aatgtcattcgagtgctctacagtaaagttcgagaatctaacaatacgtctgaagcaggt
ctgaaaaaatcggtcaaagctgcaatgatgttattgccgctgttaggtgttccgaatata
atgcaaacaattccgtttgcaccaactagggataatattatggtatttgctgtttggacg
tacaccgcctctttcacctatatgtatcaaggacttatggttgccagtatttattgcttt
acgaacaaggaggttaatcacgtcctcaagaccttctatgctcggtatcgactcctccat
aaatcacaaaacgaacttcgacgaggatcacgaagcgtcgcatcccattatgctgcaaaa
aatggaacagctaatgcaagtgcaccacagacaaataacgctgacgagtttggaaaactt
tctccgttcccgtcaaggagtaaaaaaggtagcgatgattccacgacgaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGTCATTCGAGTGCTCTACAGTA 392 161 1375 Antisense
TTGCCGCTGTTAGGTGTTCCGAATA 557 701 1468 Antisense
ACAATTCCGTTTGCACCAACTAGGG 357 113 1501 Antisense
TATTTGCTGTTTGGACGTACACCGC 608 661 1538 Antisense
CGCCTCTTTCACCTATATGTATCAA 641 257 1560 Antisense
GTTAATCACGTCCTCAAGACCTTCT 564 445 1627 Antisense
CAAGACCTTCTATGCTCGGTATCGA 240 169 1641 Antisense
TATCGACTCCTCCATAAATCACAAA 576 657 1660 Antisense
CGAGGATCACGAAGCGTCGCATCCC 97 251 1696 Antisense
CGTCGCATCCCATTATGCTGCAAAA 235 245 1710 Antisense
GGTAGCGATGATTCCACGACGAAGC 12 505 1822 Antisense
Sequence Source Affymetrix Proprietary Database