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[191897_at] tryptophanyl-tRNA synthetase

Description Y80D3A.1 /REP_DB=WormBase Gene ID /WP=CE24673 /TR=Q9U1R2 /GB=CAB60439.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=tryptophanyl-tRNA synthetase
Cluster 6grp_total > 50      4grp_total > 28      6grp_cgc > 94      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 140.7
(3)
A 		120.5
(-3)
A 		140.2
(2.9)
P 		139
(2.5)
A 		20
B 110.7
(0.1)
M 		135.7
(2.6)
A 		80.5
(-3)
M 		139.6
(3)
A 		59
C 134.6
(3)
P 		127.6
(0.7)
M 		123.6
(-0.5)
A 		116
(-3)
A 		19
avr 128.7
(2) 		127.9
(1.7) 		114.8
(-3) 		131.5
(3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 65.7
(3)
A 		61.4
(2.6)
A 		30.9
(-0.4)
A 		43.7
(0.9)
A 		4.3
(-3)
A 		55.5
(2)
A 		61
B 61.7
(2.7)
A 		42.3
(-2)
A 		61.2
(2.6)
A 		38
(-3)
A 		45.3
(-1.2)
A 		63
(3)
A 		25
C 47.5
(-0.7)
A 		32.4
(-1.5)
A 		79.9
(1)
A 		116.9
(3)
A 		3.3
(-3)
A 		25
(-1.9)
A 		114
avr 58.3
(2) 		45.4
(0.4) 		57.3
(1.9) 		66.2
(3) 		17.6
(-3) 		47.8
(0.7) 		49
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 33
1 2 3 4 range
A 51.2
(3)
A 		26.2
(-1.9)
A 		36.7
(0.2)
P 		20.4
(-3)
A 		31
B 35.3
(3)
M 		33.5
(2.3)
A 		18.7
(-3)
M 		29.8
(1)
A 		17
C 44.6
(3)
P 		36.7
(1.1)
M 		43.1
(2.6)
A 		19.6
(-3)
A 		25
avr 43.7
(3) 		32.1
(-0.4) 		32.8
(-0.2) 		23.3
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 12.1
(0.2)
A 		21.8
(3)
A 		6.7
(-1.4)
A 		11.1
(-0.1)
A 		1.3
(-3)
A 		13.5
(0.6)
A 		21
B 21.5
(2.1)
A 		14.1
(-1.2)
A 		14.9
(-0.9)
A 		10.1
(-3)
A 		18.4
(0.7)
A 		23.5
(3)
A 		13
C 17.6
(1.4)
A 		9.9
(-0.6)
A 		20.3
(2.1)
A 		23.5
(3)
A 		1.1
(-3)
A 		8.4
(-1)
A 		22
avr 17.1
(3) 		15.3
(1.9) 		14
(1.2) 		14.9
(1.7) 		6.9
(-3) 		15.1
(1.8) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191897_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13719
Sequence Type Exemplar sequence
Representative Public ID Y80D3A.1 NCBI
Target Description Y80D3A.1 /REP_DB=WormBase Gene ID /WP=CE24673 /TR=Q9U1R2 /GB=CAB60439.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=tryptophanyl-tRNA synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075282(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075282 NCBI Caenorhabditis elegans tryptophanyl (W) tRNA Synthetase family member (wrs-1) (wrs-1) mRNA, complete cds. 11/11 None
Y80D3A.1 ENSEMBL cdna:known chromosome:CEL140:V:18855308:18861794:-1 gene:Y80D3A.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000017380 3/11 Cross Hyb Matching Probes None
GENEFINDER00000017382 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18858633-18861777(-) 100.0 100.0
Public Domain and Genome References
Gene Title tryptophanyl-tRNA synthetase
Gene Symbol wrs-1
Ensembl Y80D3A.1
Entrez Gene 180231 Entrez gene
SwissProt Q9U1R2 EMBL-EBI
WormBase CE35140 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:258820_AT tRNA synthetase class I (W and Y) family protein at
CANINE:1604274_AT similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) cfa
CANINE_2:CFA.18133.1.S1_S_AT similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) cfa
CANINE_2:CFA.19799.1.S1_AT similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) cfa
CANINE_2:CFAAFFX.27413.1.S1_AT similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) cfa
CANINE_2:CFAAFFX.27416.1.S1_AT similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) cfa
CANINE_2:CFAAFFX.27416.1.S1_S_AT similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) cfa
DROSOPHILA_2:1629619_AT Tryptophanyl-tRNA synthetase dm
DROSOPHILA_2:1638511_AT Tryptophanyl-tRNA synthetase dm
DROSOPHILA_2:1635547_A_AT Tryptophanyl-tRNA synthetase dm
DROSGENOME1:149546_AT Tryptophanyl-tRNA synthetase dm
CHICKEN:GGA.11973.1.S1_AT tryptophanyl-tRNA synthetase gga
HG-U133_PLUS_2:200629_AT tryptophanyl-tRNA synthetase hs
HG-U133A:200629_AT tryptophanyl-tRNA synthetase hs
HG-FOCUS:200629_AT tryptophanyl-tRNA synthetase hs
HG-U133A_2:200629_AT tryptophanyl-tRNA synthetase hs
HG-U133A:200628_S_AT tryptophanyl-tRNA synthetase hs
HG-U133_PLUS_2:200628_S_AT tryptophanyl-tRNA synthetase hs
HG-U133A_2:200628_S_AT tryptophanyl-tRNA synthetase hs
HU35KSUBC:RC_H13689_AT tryptophanyl-tRNA synthetase hs
HUGENEFL:X59892_AT tryptophanyl-tRNA synthetase hs
U133_X3P:G7710155_3P_AT tryptophanyl-tRNA synthetase hs
U133_X3P:G340367_3P_A_AT tryptophanyl-tRNA synthetase hs
HG-U95AV2:38121_AT tryptophanyl-tRNA synthetase hs
HG-U95C:60998_AT tryptophanyl-tRNA synthetase hs
HG-U95C:66555_AT Tryptophanyl-tRNA synthetase hs
HG-U95D:71839_AT Tryptophanyl-tRNA synthetase hs
MG-U74AV2:98606_S_AT tryptophanyl-tRNA synthetase mm
MOE430A:1425106_A_AT tryptophanyl-tRNA synthetase mm
MOUSE430A_2:1425106_A_AT tryptophanyl-tRNA synthetase mm
MOUSE430_2:1425106_A_AT tryptophanyl-tRNA synthetase mm
MU11KSUBB:MSA.2538.0_S_AT tryptophanyl-tRNA synthetase mm
MU11KSUBB:MSA.23820.0_S_AT tryptophanyl-tRNA synthetase mm
MG-U74AV2:98605_AT tryptophanyl-tRNA synthetase mm
MG-U74AV2:161337_F_AT tryptophanyl-tRNA synthetase mm
MG-U74CV2:169756_R_AT tryptophanyl-tRNA synthetase mm
MG-U74CV2:169301_R_AT tryptophanyl-tRNA synthetase mm
MOE430A:1415694_AT tryptophanyl-tRNA synthetase mm
MOE430B:1415694_AT tryptophanyl-tRNA synthetase mm
MOUSE430A_2:1415694_AT tryptophanyl-tRNA synthetase mm
MOUSE430_2:1415694_AT tryptophanyl-tRNA synthetase mm
MOE430A:1434813_X_AT tryptophanyl-tRNA synthetase mm
MOUSE430A_2:1434813_X_AT tryptophanyl-tRNA synthetase mm
MOUSE430_2:1434813_X_AT tryptophanyl-tRNA synthetase mm
MOE430A:1437832_X_AT tryptophanyl-tRNA synthetase mm
MOUSE430A_2:1437832_X_AT tryptophanyl-tRNA synthetase mm
MOUSE430_2:1437832_X_AT tryptophanyl-tRNA synthetase mm
MG-U74CV2:134284_AT Tryptophanyl-tRNA synthetase, mRNA (cDNA clone MGC:6537 IMAGE:2654231) mm
MU19KSUBC:TC35977_S_AT Tryptophanyl-tRNA synthetase, mRNA (cDNA clone MGC:6537 IMAGE:2654231) mm
RG-U34B:RC_AA899491_AT tryptophanyl-tRNA synthetase (predicted) rn
RAE230A:1388574_AT tryptophanyl-tRNA synthetase (predicted) rn
RAT230_2:1388574_AT tryptophanyl-tRNA synthetase (predicted) rn
RAE230B:1395124_AT tryptophanyl-tRNA synthetase (predicted) rn
RAT230_2:1395124_AT tryptophanyl-tRNA synthetase (predicted) rn
YEAST_2:1770820_AT Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6436 tryptophanyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4830 tryptophan-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB60439 Hypothetical protein Y80D3A.1 [Caenorhabditis elegans] emb|CAE17953.1| Hypothetical protein Y80D3A.1 [Caenorhabditis elegans] ref|NP_507683.2| tryptophanyl (W) tRNA Synthetase family member (wrs-1) [Caenorhabditis elegans] dbj|BAC76735.1| tryptophanyl-tRNA synthetase (cytosolic) [Caenorhabditis elegans] 0.0
blast XP_780428 PREDICTED: similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) [Strongylocentrotus purpuratus] 1.0E-149
blast XP_868547 PREDICTED: similar to Tryptophanyl-tRNA synthetase (Tryptophan--tRNA ligase) (TrpRS) (IFP53) (hWRS) isoform 3 [Canis familiaris] 1.0E-146
Protein Domains
Database ID Description E-Value
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 3.2E-17
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 1.3E-22
Sequence
Target Sequence 
>C. ELEGANS:191897_AT
ggaagcctacatttgggccatttagtcccatttatcttcacaaaatggcttcaggaggtg
ttcgatgtgccacttgtcattcaaatgactgatgacgagaaattcttgtggaaagatatg
aaggtcgacgaggcgaaaaaaatggcccgtgaaaacatgaaagacatcatttccgtcggt
tttgatccgacaaaaaccttcattttcaacaatttcgactacatgtgcccaccgttctac
gagaatatcgtcaaaatctggaaagtcgtcaatacgaatcaggcccgagcgattttcggc
ttcacccccgaggattgtctcggaaaagcggcttttccggctgtcgaagcggctccgtgc
tttgcttcatcatttccacaaattttcggaaaacgaaacgatattccatgtctgatcccg
tgtgccatcgaccaggacccatttttccgtatgacacgtgacgtggctccaagattgaag
gcctctaagccatctttaatcttttccacattcctaccggctcttacgggagcacagacg
aagatgagcgcttctgagccgaatacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAGCCTACATTTGGGCCATTTAG 418 511 358 Antisense
GCCATTTAGTCCCATTTATCTTCAC 458 279 374 Antisense
AGGTGTTCGATGTGCCACTTGTCAT 574 55 413 Antisense
GACATCATTTCCGTCGGTTTTGATC 693 369 520 Antisense
ATCAGGCCCGAGCGATTTTCGGCTT 546 23 635 Antisense
TGCTTTGCTTCATCATTTCCACAAA 580 579 715 Antisense
AACGATATTCCATGTCTGATCCCGT 530 143 754 Antisense
CAGGACCCATTTTTCCGTATGACAC 584 201 790 Antisense
GAAGGCCTCTAAGCCATCTTTAATC 60 333 834 Antisense
ATCTTTAATCTTTTCCACATTCCTA 22 33 849 Antisense
GATGAGCGCTTCTGAGCCGAATACG 11 411 900 Antisense
Sequence Source Affymetrix Proprietary Database