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[191892_at]

Description ZK1290.4 /REP_DB=WormBase Gene ID /WP=CE02083 /GEN=nfi-1 /TR=Q09631 /GB=AAB93320.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Nuclear factor 1
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 21      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 62      6grp_total > 32      4grp_total > 31      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 255
1 2 3 4 range
A 513.9
(3)
P 		331.9
(-1.7)
P 		279.7
(-3)
P 		497.7
(2.6)
P 		234
B 384
(3)
P 		266
(-2.7)
P 		259.1
(-3)
P 		321.4
(-0)
P 		125
C 482.2
(3)
P 		299.4
(-1.9)
P 		260.2
(-3)
P 		345.9
(-0.7)
P 		222
avr 460
(3) 		299.1
(-2) 		266.3
(-3) 		388.3
(0.8) 		194

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 368
1 2 3 4 5 6 range
A 602.4
(1.3)
P 		495.9
(-0.6)
P 		699.7
(3)
P 		392.9
(-2.4)
P 		402.1
(-2.2)
P 		357
(-3)
P 		343
B 492
(1.3)
P 		552.8
(3)
P 		549.6
(2.9)
P 		543.3
(2.7)
P 		372.8
(-2)
P 		338.6
(-3)
P 		214
C 499.5
(1)
P 		509
(1.2)
P 		461.5
(0.1)
P 		586.3
(3)
P 		331.7
(-3)
P 		338.6
(-2.8)
P 		255
avr 531.3
(2) 		519.2
(1.6) 		570.3
(3) 		507.5
(1.3) 		368.9
(-2.4) 		344.7
(-3) 		226
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 187.1
(3)
P 		72
(-3)
P 		73.1
(-2.9)
P 		72.8
(-3)
P 		115
B 122.4
(3)
P 		65.7
(-2.5)
P 		60
(-3)
P 		68.5
(-2.2)
P 		62
C 159.6
(3)
P 		86.1
(-1.4)
P 		90.7
(-1.1)
P 		58.3
(-3)
P 		101
avr 156.4
(3) 		74.6
(-2.5) 		74.6
(-2.5) 		66.5
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 98
1 2 3 4 5 6 range
A 111.1
(-1.4)
P 		176.4
(3)
P 		152.4
(1.4)
P 		99.6
(-2.1)
P 		121.3
(-0.7)
P 		86.9
(-3)
P 		90
B 171.2
(1.7)
P 		184
(3)
P 		133.5
(-2.3)
P 		143.9
(-1.2)
P 		151.5
(-0.4)
P 		126.3
(-3)
P 		58
C 185.1
(3)
P 		155.3
(0.7)
P 		117.5
(-2.2)
P 		117.9
(-2.2)
P 		107.3
(-3)
P 		113.4
(-2.5)
P 		78
avr 155.8
(1.5) 		171.9
(3) 		134.5
(-0.6) 		120.5
(-1.9) 		126.7
(-1.3) 		108.9
(-3) 		63
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191892_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5272
Sequence Type Exemplar sequence
Representative Public ID ZK1290.4 NCBI
Target Description ZK1290.4 /REP_DB=WormBase Gene ID /WP=CE02083 /GEN=nfi-1 /TR=Q09631 /GB=AAB93320.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Nuclear factor 1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027334(11), NM_001027336(11), NM_001027335(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027334 NCBI Caenorhabditis elegans NFI (Nuclear Factor I) family member (nfi-1) (nfi-1) mRNA, complete cds. 11/11 None
NM_001027336 NCBI Caenorhabditis elegans NFI (Nuclear Factor I) family member (nfi-1) (nfi-1) mRNA, complete cds. 11/11 None
NM_001027335 NCBI Caenorhabditis elegans NFI (Nuclear Factor I) family member (nfi-1) (nfi-1) mRNA, complete cds. 10/11 None
SNAP00000002675 ENSEMBL cdna:SNAP chromosome:CEL140:II:7538517:7542461:1 11/11 None
GENEFINDER00000002686 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7534426:7542461:1 11/11 None
ZK1290.4c ENSEMBL cdna:known chromosome:CEL140:II:7538117:7542927:1 gene:ZK1290.4 11/11 None
ZK1290.4a ENSEMBL cdna:known chromosome:CEL140:II:7538161:7542461:1 gene:ZK1290.4 11/11 None
ZK1290.4b ENSEMBL cdna:known chromosome:CEL140:II:7538626:7542461:1 gene:ZK1290.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7534422-7542458(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK1290.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN60509 Nfi (nuclear factor i) family protein 1, isoform a [Caenorhabditis elegans] ref|NP_001022505.1| NFI (Nuclear Factor I) family member (nfi-1) [Caenorhabditis elegans] 0.0
blast AAW57823 Nfi (nuclear factor i) family protein 1, isoform c [Caenorhabditis elegans] ref|NP_001022507.1| NFI (Nuclear Factor I) family member (nfi-1) [Caenorhabditis elegans] 0.0
blast AAW57822 Nfi (nuclear factor i) family protein 1, isoform b [Caenorhabditis elegans] ref|NP_001022506.1| NFI (Nuclear Factor I) family member (nfi-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 6.5E-40
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 6.5E-40
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 6.5E-40
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 6.5E-40
Pfam IPR003619 EMBL-EBI Dwarfin protein, domain A 6.5E-40
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000002686 3 126-148,153-175,187-209
Sequence
Target Sequence 
>C. ELEGANS:191892_AT
aatcaaccgagcacgagctactgtaattcacaaatccaaccaccaatcttatcatcgaaa
ccagttgattcatcagtgaaattaattgctccagttgcagtgaaaccaattatgtcagga
tgtaattcaatcattccatcacccatcacaactcctcgtataactccttcatttcgaatg
cttgaagatgattcattgattaatgtacttggacaacttgcacacagtaatgacggaaca
actttaaacgatagcttcattcaacatcttattgataccaattctcgatctccattgctt
agtagtggaaatgctttttcggcattatcaatgggagctcggatcgaattcgttgggtgt
agcaatgggattggccgttcctcaaaatattgcactggccgtccagcaaactcaaaatgc
aatgtcacctcttcatcaagagctgcaaatcaagcaccggtctcaaatactccacaagat
ccgaatgctccaaagctgccaactgact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCAACCGAGCACGAGCTACTGTA 563 169 2554 Antisense
ACCACCAATCTTATCATCGAAACCA 72 87 2592 Antisense
TCCTCGTATAACTCCTTCATTTCGA 501 591 2706 Antisense
ATTCTCGATCTCCATTGCTTAGTAG 681 9 2834 Antisense
GGGAGCTCGGATCGAATTCGTTGGG 511 493 2886 Antisense
GTAGCAATGGGATTGGCCGTTCCTC 383 457 2912 Antisense
TATTGCACTGGCCGTCCAGCAAACT 638 663 2941 Antisense
AAATGCAATGTCACCTCTTCATCAA 692 119 2968 Antisense
TCAAGCACCGGTCTCAAATACTCCA 596 631 3003 Antisense
TACTCCACAAGATCCGAATGCTCCA 274 645 3021 Antisense
AATGCTCCAAAGCTGCCAACTGACT 70 167 3037 Antisense
Sequence Source Affymetrix Proprietary Database