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[191874_s_at] guanylate cyclase

Description F57F5.2 /REP_DB=WormBase Gene ID /WP=CE20887 /GEN=gcy-33 /TR=P90895 /GB=CAB01418.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster 6grp_total > 45      4grp_total > 21      6grp_cgc > 35      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 150
1 2 3 4 range
A 74.6
(0.5)
M 		43.2
(-2.2)
A 		33
(-3)
A 		105.3
(3)
A 		72
B 87
(0.2)
A 		63.4
(-0.7)
A 		6.5
(-3)
A 		156.6
(3)
P 		150
C 113.9
(2.5)
M 		91.1
(0.3)
A 		56.6
(-3)
A 		118.7
(3)
A 		62
avr 91.8
(0.8) 		65.9
(-0.9) 		32
(-3) 		126.9
(3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 78.6
(0.4)
A 		79.4
(0.5)
A 		79.2
(0.5)
A 		98
(3)
P 		52.9
(-3)
A 		87.1
(1.5)
A 		45
B 89.7
(-0.1)
P 		117.8
(2.7)
P 		120.8
(3)
P 		60.8
(-3)
A 		65.5
(-2.5)
A 		72.2
(-1.9)
P 		60
C 100.9
(2.2)
P 		106.2
(3)
P 		91.2
(0.7)
P 		67.6
(-3)
A 		67.3
(-3)
A 		90.8
(0.6)
P 		39
avr 89.7
(1.3) 		101.1
(3) 		97.1
(2.4) 		75.5
(-0.9) 		61.9
(-3) 		83.4
(0.3) 		39
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 27.2
(3)
M 		9.4
(-2.7)
A 		8.6
(-3)
A 		15.4
(-0.8)
A 		19
B 27.8
(1.9)
A 		15.7
(-0.3)
A 		1.5
(-3)
A 		33.4
(3)
P 		32
C 37.7
(3)
M 		26.2
(-0.8)
A 		19.7
(-3)
A 		20
(-2.9)
A 		18
avr 30.9
(3) 		17.1
(-0.9) 		9.9
(-3) 		22.9
(0.7) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 14.5
(-3)
A 		28.2
(3)
A 		17.2
(-1.8)
A 		24.9
(1.6)
P 		16
(-2.3)
A 		21.2
(-0.1)
A 		14
B 31.2
(0.9)
P 		39.2
(3)
P 		29.3
(0.4)
P 		16.1
(-3)
A 		26.6
(-0.3)
A 		26.9
(-0.2)
P 		23
C 37.4
(3)
P 		32.4
(1.7)
P 		23.2
(-0.6)
P 		13.6
(-3)
A 		21.8
(-0.9)
A 		30.4
(1.2)
P 		24
avr 27.7
(0.8) 		33.3
(3) 		23.2
(-1) 		18.2
(-3) 		21.5
(-1.7) 		26.2
(0.2) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191874_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13829
Sequence Type Exemplar sequence
Representative Public ID F57F5.2 NCBI
Target Description F57F5.2 /REP_DB=WormBase Gene ID /WP=CE20887 /GEN=gcy-33 /TR=P90895 /GB=CAB01418.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073609(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073609 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-33) (gcy-33) mRNA, complete cds. 11/11 A 
F57F5.2 ENSEMBL cdna:known chromosome:CEL140:V:11998456:12004364:-1 gene:F57F5.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11998455-12004364(-) 100.0 100.0
Public Domain and Genome References
Gene Title guanylate cyclase
Gene Symbol gcy-33
Ensembl F57F5.2
Entrez Gene 179644 Entrez gene
WormBase CE34891 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01418 Hypothetical protein F57F5.2 [Caenorhabditis elegans] emb|CAB00103.2| Hypothetical protein F57F5.2 [Caenorhabditis elegans] ref|NP_506010.2| Guanylyl CYclase family member (gcy-33) [Caenorhabditis elegans] gb|AAP32289.1| soluble guanylyl cyclase GCY-33 [Caenorhabditis elegans] sp|P90895|GCY33_CAEEL Soluble guanylate cyclase gcy-33 0.0
blast CAE75366 Hypothetical protein CBG23350 [Caenorhabditis briggsae] 0.0
blast BAD89804.1 soluble guanylyl cyclase beta-3 [Apis mellifera] ref|NP_001012645.1| soluble guanylyl cyclase beta-3 [Apis mellifera] 1.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 2.1E-41
Sequence
Target Sequence 
>C. ELEGANS:191874_S_AT
gtcgtttgagttcctctgatttggcagttagtcgtgtagaaacatctaaagattctgatg
gtgaaactccccggccaacgtctagcgaactaaaagaagtcaatcggatccgtgaagaag
cacttgctcaagaaaaggaagaagagagaacaacgaaagaagagaatcagaaaatagaag
aagtgggggaggatcatgtcagtgaagcaacctcacttcttgactctgaggtcagtcacg
gcgataacaacatcagtttctctcaaatgccgtctgatagcattcctcacgaagatcgga
cttctcttccttcagcgacaccttcagaaataggagatgcaatttccaaaaaaaaattgg
aaaaggaagattcaaactcatcgatgagcagtttggacgaaagaacaactgtgtctgcaa
aacctaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGTTTGAGTTCCTCTGATTTGGC 435 473 2228 Antisense
CTGATTTGGCAGTTAGTCGTGTAGA 559 237 2243 Antisense
GGATCCGTGAAGAAGCACTTGCTCA 632 511 2333 Antisense
GAAGTGGGGGAGGATCATGTCAGTG 94 335 2407 Antisense
GTGAAGCAACCTCACTTCTTGACTC 644 469 2429 Antisense
TCACTTCTTGACTCTGAGGTCAGTC 463 621 2440 Antisense
GAGGTCAGTCACGGCGATAACAACA 595 401 2455 Antisense
GCGATAACAACATCAGTTTCTCTCA 317 291 2468 Antisense
GTTTCTCTCAAATGCCGTCTGATAG 278 451 2483 Antisense
ACGAAGATCGGACTTCTCTTCCTTC 209 93 2516 Antisense
AACAACTGTGTCTGCAAAACCTACA 431 133 2631 Antisense
Sequence Source Affymetrix Proprietary Database