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[191861_at] RAS-like GTP-binding protein

Description Y32F6B.3 /REP_DB=WormBase Gene ID /WP=CE16612 /TR=O62422 /GB=CAA16313.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=RAS-like GTP-binding protein
Cluster 6grp_highest_10_10 > 06      6grp_high1_10_10 > 51      4grp_highest_8_8 > 60      4grp_high_8_8 > 26      6grp_highest_nonorm > 62      6grp_total > 61      4grp_total > 38      6grp_highest_sub > 04      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 359
1 2 3 4 range
A 431.9
(2.1)
P 		457.2
(3)
P 		287.5
(-3)
P 		353.3
(-0.7)
P 		170
B 550.7
(3)
P 		465.2
(-0.7)
P 		412.5
(-3)
P 		470.9
(-0.5)
P 		138
C 646.9
(3)
P 		419.6
(-2.5)
P 		396.9
(-3)
P 		471.3
(-1.2)
P 		250
avr 543.2
(3) 		447.3
(-0.2) 		365.6
(-3) 		431.8
(-0.8) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 510
1 2 3 4 5 6 range
A 528.2
(0.4)
P 		471.9
(-0.3)
P 		752.5
(3)
P 		637.7
(1.7)
P 		428.8
(-0.8)
P 		242.2
(-3)
P 		510
B 432.4
(-1)
P 		581.8
(1.6)
P 		647.1
(2.7)
P 		664.7
(3)
P 		487
(-0)
P 		312.6
(-3)
P 		352
C 436.2
(-2.4)
P 		695
(3)
P 		424.7
(-2.7)
P 		651.9
(2.1)
P 		458.4
(-1.9)
P 		408.2
(-3)
P 		287
avr 465.6
(-0.4) 		582.9
(1.8) 		608.1
(2.2) 		651.4
(3) 		458.1
(-0.5) 		321
(-3) 		330
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 162
1 2 3 4 range
A 157.2
(3)
P 		99.2
(-0.3)
P 		75.2
(-1.7)
P 		51.7
(-3)
P 		106
B 175.6
(3)
P 		114.9
(-1.6)
P 		95.6
(-3)
P 		100.4
(-2.6)
P 		80
C 214.1
(3)
P 		120.6
(-1.2)
P 		138.3
(-0.4)
P 		79.5
(-3)
P 		135
avr 182.3
(3) 		111.6
(-1) 		103
(-1.5) 		77.2
(-3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 97.4
(-0.9)
P 		167.8
(3)
P 		163.9
(2.8)
P 		161.7
(2.7)
P 		129.4
(0.9)
P 		59
(-3)
P 		109
B 150.4
(-0.5)
P 		193.6
(2.7)
P 		157.1
(-0)
P 		176.1
(1.4)
P 		197.9
(3)
P 		116.6
(-3)
P 		81
C 161.7
(0.1)
P 		212.1
(3)
P 		108.1
(-3)
P 		131.1
(-1.7)
P 		148.3
(-0.7)
P 		136.7
(-1.4)
P 		104
avr 136.5
(-0.8) 		191.2
(3) 		143
(-0.3) 		156.3
(0.6) 		158.5
(0.7) 		104.1
(-3) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191861_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11875
Sequence Type Exemplar sequence
Representative Public ID Y32F6B.3 NCBI
Target Description Y32F6B.3 /REP_DB=WormBase Gene ID /WP=CE16612 /TR=O62422 /GB=CAA16313.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=RAS-like GTP-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073287(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073287 NCBI Caenorhabditis elegans Cdc-42 Related Protein family member (crp-1) (crp-1) mRNA, complete cds. 11/11 None
GENEFINDER00000025296 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10481348:10483334:1 11/11 None
Y32F6B.3 ENSEMBL cdna:known chromosome:CEL140:V:10481172:10483529:1 gene:Y32F6B.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10481347-10483334(+) 100.0 100.0
Public Domain and Genome References
Gene Title RAS-like GTP-binding protein
Gene Symbol crp-1
Ensembl Y32F6B.3
Entrez Gene 179462 Entrez gene
SwissProt O62422 EMBL-EBI
WormBase CE16612 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA16313 Hypothetical protein Y32F6B.3 [Caenorhabditis elegans] ref|NP_505688.1| Cdc-42 Related Protein family member (crp-1) [Caenorhabditis elegans] 1.0E-107
blast CAE64808 Hypothetical protein CBG09601 [Caenorhabditis briggsae] 5.0E-92
blast AAX55504 small GTPase Cd42 [Schizophyllum commune] gb|AAK77967.2| small GTPase CDC42 [Schizophyllum commune] 1.0E-38
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 7.5E-46
Sequence
Target Sequence 
>C. ELEGANS:191861_AT
agtcgtgggggatacctatactggtaaaacaagtcttcttgtcgcttacacgaaaaaaca
atttctggataactacacaactactgtatttgataactgggctgtttcagttcatattga
caataagaactatgctgtcaatttatttgatactgctggacagggaaactatgaacaaat
tcgatgtctgagttatccacatgcaaatgtctttcttgtttgtttttcaatgattgacag
gaaaacgttagaatcgtgcagaagtacgtggatacctgaaattcggaaatatgctggtga
taatgttcctataatgttagttgggacaaaaaatgacctcgtggatgacgctgattctcg
gaatacagtgacagaagattatgctaaaagagtcgctcatgagatcggttgtcacaaatt
ctattcatgttctgcgctgacgcacaaaggcttaaaacgtgtatttgatgaaagcttcct
ggctgctgtaggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTCGTGGGGGATACCTATACTGGT 156 59 36 Antisense
AAGTCTTCTTGTCGCTTACACGAAA 307 157 66 Antisense
AATTTCTGGATAACTACACAACTAC 88 173 95 Antisense
GTCTGAGTTATCCACATGCAAATGT 540 471 221 Antisense
GTACGTGGATACCTGAAATTCGGAA 253 457 299 Antisense
ATATGCTGGTGATAATGTTCCTATA 483 21 324 Antisense
GACCTCGTGGATGACGCTGATTCTC 463 383 370 Antisense
GTCGCTCATGAGATCGGTTGTCACA 625 473 427 Antisense
CAAATTCTATTCATGTTCTGCGCTG 70 195 450 Antisense
GTTCTGCGCTGACGCACAAAGGCTT 309 443 464 Antisense
TGAAAGCTTCCTGGCTGCTGTAGGT 698 571 504 Antisense
Sequence Source Affymetrix Proprietary Database