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[191856_s_at] myosin heavy chain

Description K12F2.1 /REP_DB=WormBase Gene ID /WP=CE12204 /GEN=myo-3 /TR=Q21440 /GB=CAB01576.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=myosin heavy chain
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 05      6grp_highest_nonorm > 20      6grp_total > 06      4grp_total > 59      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1693
1 2 3 4 range
A 866.1
(-0)
P 		1715.2
(3)
P 		1508.5
(2.3)
P 		22
(-3)
A 		1693
B 810.1
(0)
P 		1465.9
(3)
P 		1299.3
(2.2)
P 		147.8
(-3)
A 		1318
C 824.4
(-0.1)
P 		1573.6
(3)
P 		1404
(2.3)
P 		112.9
(-3)
A 		1461
avr 833.5
(-0) 		1584.9
(3) 		1403.9
(2.3) 		94.2
(-3) 		1491

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2315
1 2 3 4 5 6 range
A 1775.5
(3)
P 		1769
(3)
P 		760.8
(-2.1)
P 		768.7
(-2.1)
P 		582.3
(-3)
P 		646.7
(-2.7)
P 		1193
B 1566.6
(3)
P 		1227.6
(1.3)
P 		922.6
(-0.2)
P 		680.1
(-1.4)
P 		660.4
(-1.5)
P 		362.1
(-3)
P 		1205
C 2676.9
(3)
P 		1135
(-1.1)
P 		1214
(-0.9)
P 		580.7
(-2.6)
P 		1091.5
(-1.2)
P 		427.1
(-3)
P 		2250
avr 2006.3
(3) 		1377.2
(0.5) 		965.8
(-1.1) 		676.5
(-2.2) 		778.1
(-1.8) 		478.6
(-3) 		1528
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 486
1 2 3 4 range
A 315.3
(1.8)
P 		372.3
(2.7)
P 		394.4
(3)
P 		3.2
(-3)
A 		391
B 258.3
(1.1)
P 		362
(3)
P 		301.1
(1.9)
P 		31.5
(-3)
A 		331
C 272.9
(0.2)
P 		452.4
(2.5)
P 		489.3
(3)
P 		19
(-3)
A 		470
avr 282.2
(1.2) 		395.6
(3) 		394.9
(3) 		17.9
(-3) 		378

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 875
1 2 3 4 5 6 range
A 327.3
(-0.8)
P 		629.1
(3)
P 		165.7
(-2.9)
P 		195
(-2.5)
P 		175.7
(-2.8)
P 		157.4
(-3)
P 		472
B 545.1
(3)
P 		408.6
(1)
P 		224
(-1.7)
P 		180.1
(-2.3)
P 		268.3
(-1)
P 		135
(-3)
P 		410
C 992.2
(3)
P 		346.3
(-1.4)
P 		309
(-1.7)
P 		116.8
(-3)
P 		353.2
(-1.4)
P 		143
(-2.8)
P 		875
avr 621.5
(3) 		461.3
(1) 		232.9
(-1.9) 		164
(-2.8) 		265.7
(-1.5) 		145.1
(-3) 		476
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191856_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14664
Sequence Type Exemplar sequence
Representative Public ID K12F2.1 NCBI
Target Description K12F2.1 /REP_DB=WormBase Gene ID /WP=CE12204 /GEN=myo-3 /TR=Q21440 /GB=CAB01576.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=myosin heavy chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073664(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073664 NCBI Caenorhabditis elegans MYOsin heavy chain structural genes family member (myo-3) (myo-3) mRNA, complete cds. 11/11 None
SNAP00000012838 ENSEMBL cdna:SNAP chromosome:CEL140:V:12229556:12236888:-1 11/11 None
GENEFINDER00000012839 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12229556:12236888:-1 11/11 None
K12F2.1 ENSEMBL cdna:known chromosome:CEL140:V:12229407:12236888:-1 gene:K12F2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12229555-12236888(-) 100.0 100.0
Public Domain and Genome References
Gene Title myosin heavy chain
Gene Symbol myo-3
Ensembl K12F2.1
Entrez Gene 179676 Entrez gene
SwissProt P12844 EMBL-EBI
WormBase CE34936 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23235.1.S1_AT masticatory myosin heavy chain 16 cfa
CANINE_2:CFAAFFX.23238.1.S1_AT masticatory myosin heavy chain 16 cfa
CANINE_2:CFAAFFX.23241.1.S1_AT masticatory myosin heavy chain 16 cfa
CANINE_2:CFAAFFX.23243.1.S1_AT masticatory myosin heavy chain 16 cfa
CANINE_2:CFAAFFX.23245.1.S1_AT masticatory myosin heavy chain 16 cfa
CANINE_2:CFAAFFX.23247.1.S1_AT masticatory myosin heavy chain 16 cfa
CANINE_2:CFAAFFX.23265.1.S1_AT masticatory myosin heavy chain 16 cfa
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01576 Hypothetical protein K12F2.1 [Caenorhabditis elegans] emb|CAA30856.1| myosin heavy chain 3 [Caenorhabditis elegans] ref|NP_506065.2| MYOsin heavy chain structural genes family member (myo-3) [Caenorhabditis elegans] sp|P12844|MYO3_CAEEL Myosin-3 (Myosin heavy chain A) (MHC A) 0.0
blast CAE75423 Hypothetical protein CBG23416 [Caenorhabditis briggsae] 0.0
blast CAE72546 Hypothetical protein CBG19730 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 7.69999980926514
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 1.6E-24
Pfam IPR002928 EMBL-EBI Myosin tail 1.0E-126
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191856_S_AT
caagaacgccgttgaacaaggacaaaaggcttcagccgatgctgcccgtcttgccgaaga
gctccgtcaagaacaagagcactccatgcacattgaaagaatccgcaagggacttgaact
tcaaatcaaggaaatgcagatcagacttgatgatgctgaaaatgcggcattgaagggagg
aaagaagatcattgctcaattggaagccagaattcgtgcaattgaacaagaattggatgg
agaacagagacgtcatcaagacaccgagaagaactggagaaaggctgaaagacgtgtcaa
ggaggttgaattccaagttgtcgaggagaagaagaatgaagaaagactgacggaattggt
tgacaagcttcaatgcaaacttaagatcttcaagagacaggttgaggaggctgaggaagt
tgccgccagcaacttgaacaagtacaaggttctcactgctcaatttgaacaagctgaaga
gagagccgatattgctgaaaatgccctgtcaaagatgcgtaacaagattcgtgcatcagc
t

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGAACGCCGTTGAACAAGGACAA 572 185 5364 Antisense
CAAAAGGCTTCAGCCGATGCTGCCC 255 191 5386 Antisense
GTCTTGCCGAAGAGCTCCGTCAAGA 238 469 5411 Antisense
GAACAAGAGCACTCCATGCACATTG 181 353 5434 Antisense
GAATCCGCAAGGGACTTGAACTTCA 59 329 5462 Antisense
GGAAGCCAGAATTCGTGCAATTGAA 85 531 5565 Antisense
GAACAGAGACGTCATCAAGACACCG 336 349 5605 Antisense
GAAAGACTGACGGAATTGGTTGACA 471 359 5704 Antisense
TTGCCGCCAGCAACTTGAACAAGTA 254 699 5783 Antisense
AGTACAAGGTTCTCACTGCTCAATT 430 61 5804 Antisense
GCGTAACAAGATTCGTGCATCAGCT 14 293 5880 Antisense
Sequence Source Affymetrix Proprietary Database