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[191852_at] guanylate cyclase

Description C06B3.8 /REP_DB=WormBase Gene ID /WP=CE07965 /GEN=gcy-32 /TR=Q17707 /GB=CAB01118.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster 6grp_total > 14      4grp_total > 29      6grp_cgc > 40      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 72.7
(2)
P 		70.6
(1.8)
A 		24.5
(-3)
A 		81.9
(3)
A 		57
B 71
(3)
P 		57.9
(1.8)
A 		7.6
(-3)
A 		40.4
(0.1)
A 		63
C 99.2
(3)
P 		40.4
(-1.6)
A 		22.3
(-3)
A 		86
(2)
P 		77
avr 81
(3) 		56.3
(0.6) 		18.1
(-3) 		69.4
(1.9) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 38.6
(1.6)
A 		48.9
(3)
P 		16.9
(-1.3)
A 		16.2
(-1.4)
A 		4.4
(-3)
A 		36.2
(1.3)
A 		45
B 57.2
(0.5)
P 		57
(0.5)
P 		54.1
(0)
A 		73.9
(3)
P 		35.1
(-2.8)
A 		34.1
(-3)
A 		40
C 81.5
(3)
P 		63.2
(1.1)
P 		37
(-1.5)
A 		57.9
(0.6)
A 		31.5
(-2.1)
A 		22.3
(-3)
A 		59
avr 59.1
(3) 		56.4
(2.5) 		36
(-0.9) 		49.3
(1.3) 		23.7
(-3) 		30.9
(-1.8) 		35
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 26.5
(3)
P 		15.3
(-0.3)
A 		6.4
(-3)
A 		12
(-1.3)
A 		20
B 22.6
(3)
P 		14.3
(0.6)
A 		1.8
(-3)
A 		8.6
(-1)
A 		21
C 32.8
(3)
P 		11.6
(-2.1)
A 		7.8
(-3)
A 		14.5
(-1.4)
P 		25
avr 27.3
(3) 		13.7
(-0.7) 		5.3
(-3) 		11.7
(-1.3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 7.1
(-0.8)
A 		17.4
(3)
P 		3.7
(-2.1)
A 		4.1
(-2)
A 		1.3
(-3)
A 		8.8
(-0.2)
A 		16
B 19.9
(3)
P 		19
(2.3)
P 		13.1
(-2.7)
A 		19.6
(2.8)
P 		14.3
(-1.7)
A 		12.7
(-3)
A 		7
C 30.2
(3)
P 		19.3
(0.1)
P 		9.4
(-2.5)
A 		11.6
(-1.9)
A 		10.2
(-2.3)
A 		7.5
(-3)
A 		23
avr 19.1
(3) 		18.6
(2.7) 		8.7
(-2.9) 		11.8
(-1.2) 		8.6
(-3) 		9.7
(-2.4) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191852_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13773
Sequence Type Exemplar sequence
Representative Public ID C06B3.8 NCBI
Target Description C06B3.8 /REP_DB=WormBase Gene ID /WP=CE07965 /GEN=gcy-32 /TR=Q17707 /GB=CAB01118.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074051(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074051 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-32) (gcy-32) mRNA, complete cds. 11/11 None
SNAP00000008245 ENSEMBL cdna:SNAP chromosome:CEL140:V:13914759:13917667:-1 11/11 None
GENEFINDER00000008250 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13914759:13917667:-1 11/11 None
C06B3.8.2 ENSEMBL cdna:known chromosome:CEL140:V:13914669:13918025:-1 gene:C06B3.8 11/11 None
C06B3.8.1 ENSEMBL cdna:known chromosome:CEL140:V:13914670:13917779:-1 gene:C06B3.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13914758-13917779(-) 99.9 99.9
Public Domain and Genome References
Gene Title guanylate cyclase
Gene Symbol gcy-32
Ensembl C06B3.8
Entrez Gene 179887 Entrez gene
SwissProt Q6DNF7 EMBL-EBI
WormBase CE30594 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4383 guanylate cyclase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01118 Hypothetical protein C06B3.8 [Caenorhabditis elegans] ref|NP_506452.4| Guanylyl CYclase family member (gcy-32) [Caenorhabditis elegans] 0.0
blast AAT73709 guanylate cyclase-like protein [Caenorhabditis elegans] sp|Q6DNF7|GCY32_CAEEL Soluble guanylate cyclase gcy-32 0.0
blast CAC35530 soluble guanylate cyclase [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.6E-53
Sequence
Target Sequence 
>C. ELEGANS:191852_AT
aattgttgttattcgaggtgtctacaaagtggagacagtgtcggacagttacatggcagt
atccggtattccagactatacacctgaacacgctgaaaacatgtgtcacgtggcattggg
aatgatgtgggaggcaagaagtgtgatagatcccgtgtcaaaaactccattcttgttgag
aattggcattcactcaggaactattacagctggagttgttgggacagtacatccgaaata
ctgtttattcggtgaaacagtaactttggcatctcaaatggaatctcttggaatggctgg
aaagattcaatgtagtaaatgggcatatcagaaagcaatggaaactggaagatttgaatt
ttccccacgtgggagaattgatgtgaagcaaagaggtcttacggaaacgtattttttgac
gagaagtttgaagaaaagtatttgggaaattattgatcatgatagagatattaacgtgaa
cagtatcgaaggttatgaagagttggaaacagcaattgagaacgctgtaactataaagtc
ggcacttccaagaccagatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTGTTGTTATTCGAGGTGTCTAC 393 177 1521 Antisense
AGTGTCGGACAGTTACATGGCAGTA 559 57 1557 Antisense
GGCAGTATCCGGTATTCCAGACTAT 136 535 1575 Antisense
GACTATACACCTGAACACGCTGAAA 479 371 1594 Antisense
ACGCTGAAAACATGTGTCACGTGGC 487 91 1610 Antisense
AGATCCCGTGTCAAAAACTCCATTC 68 65 1668 Antisense
GAATTGGCATTCACTCAGGAACTAT 660 321 1700 Antisense
GGAGTTGTTGGGACAGTACATCCGA 631 517 1732 Antisense
GGTGAAACAGTAACTTTGGCATCTC 285 501 1771 Antisense
GAAGATTTGAATTTTCCCCACGTGG 27 337 1868 Antisense
AAGTCGGCACTTCCAAGACCAGATC 706 155 2056 Antisense
Sequence Source Affymetrix Proprietary Database