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[191851_at] phosphatidylserine synthase I

Description T27E9.5 /REP_DB=WormBase Gene ID /WP=CE24019 /TR=O18143 /GB=CAB04876.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=phosphatidylserine synthase I
Cluster 6grp_total > 87      4grp_total > 24      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 76
(-3)
A 		80.8
(-2.7)
A 		148.2
(1)
M 		185
(3)
P 		109
B 68.1
(-3)
A 		121.9
(2.2)
P 		129.8
(3)
P 		110.3
(1.1)
P 		62
C 62.7
(-3)
A 		116.7
(0.4)
A 		105.1
(-0.3)
P 		158.6
(3)
P 		96
avr 68.9
(-3) 		106.5
(-0.3) 		127.7
(1.3) 		151.3
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 458
1 2 3 4 5 6 range
A 33.5
(-3)
A 		54.8
(-2.7)
A 		116
(-1.8)
P 		120.9
(-1.7)
P 		262.2
(0.3)
P 		443.4
(3)
P 		410
B 72.1
(-2.8)
A 		61.1
(-3)
A 		108
(-2.3)
P 		131.5
(-2)
P 		237.6
(-0.5)
P 		491.2
(3)
P 		430
C 47.8
(-3)
A 		57.8
(-2.8)
A 		106.7
(-2.1)
A 		173.4
(-1)
P 		229.9
(-0.1)
P 		421.3
(3)
P 		374
avr 51.1
(-3) 		57.9
(-2.9) 		110.2
(-2.1) 		141.9
(-1.6) 		243.2
(-0.1) 		452
(3) 		401
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 27.7
(-0.1)
A 		17.5
(-3)
A 		38.8
(3)
M 		27.1
(-0.3)
P 		21
B 21.7
(-3)
A 		30.1
(3)
P 		30.1
(3)
P 		23.5
(-1.7)
P 		8
C 20.8
(-3)
A 		33.5
(1.8)
A 		36.6
(3)
P 		26.7
(-0.8)
P 		16
avr 23.4
(-3) 		27
(-1.2) 		35.2
(3) 		25.8
(-1.8) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 177
1 2 3 4 5 6 range
A 6.2
(-3)
A 		19.5
(-2.2)
A 		25.3
(-1.9)
P 		30.7
(-1.6)
P 		79.1
(1.3)
P 		108
(3)
P 		102
B 25.1
(-2.8)
A 		20.3
(-3)
A 		26.2
(-2.8)
P 		34.8
(-2.5)
P 		96.5
(-0.2)
P 		183.2
(3)
P 		163
C 17.7
(-3)
A 		17.6
(-3)
A 		27.2
(-2.5)
A 		34.9
(-2.2)
P 		74.4
(-0.2)
P 		141
(3)
P 		123
avr 16.3
(-3) 		19.1
(-2.9) 		26.2
(-2.5) 		33.5
(-2.2) 		83.3
(0.1) 		144.1
(3) 		128
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191851_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6850
Sequence Type Exemplar sequence
Representative Public ID T27E9.5 NCBI
Target Description T27E9.5 /REP_DB=WormBase Gene ID /WP=CE24019 /TR=O18143 /GB=CAB04876.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=phosphatidylserine synthase I
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067385(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067385 NCBI Caenorhabditis elegans T27E9.5 (T27E9.5) mRNA, complete cds. 11/11 None
GENEFINDER00000014280 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13471389:13474832:1 11/11 None
T27E9.5 ENSEMBL cdna:known chromosome:CEL140:III:13471224:13474854:1 gene:T27E9.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000014266 2/11 Cross Hyb Matching Probes None
SNAP00000014268 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13471219-13474828(+) 97.92 100.0
Public Domain and Genome References
Gene Title phosphatidylserine synthase I
Ensembl T27E9.5
Entrez Gene 176776 Entrez gene
SwissProt O18143 EMBL-EBI
WormBase CE24019 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6659 phosphatidylserine biosynthesis inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04876 Hypothetical protein T27E9.5 [Caenorhabditis elegans] ref|NP_499786.1| T27E9.5 [Caenorhabditis elegans] 0.0
blast CAE69270 Hypothetical protein CBG15323 [Caenorhabditis briggsae] 0.0
blast CAE69270 Hypothetical protein CBG15323 [Caenorhabditis briggsae] 1.0E-163
blast CAA87373 Hypothetical protein ZC506.3 [Caenorhabditis elegans] ref|NP_509673.1| ZC506.3 [Caenorhabditis elegans] 1.0E-142
blast CAA87373 Hypothetical protein ZC506.3 [Caenorhabditis elegans] ref|NP_509673.1| ZC506.3 [Caenorhabditis elegans] 3.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR004277 EMBL-EBI Phosphatidyl serine synthase 1.0E-126
Pfam IPR004277 EMBL-EBI Phosphatidyl serine synthase 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499786.1 9 32-54,144-161,174-196,200-222,282-304,319-341,441-463,478-500,507-529
T27E9.5 9 32-54,144-161,174-196,200-222,282-304,319-341,441-463,478-500,507-529
GENEFINDER00000014280 6 89-106,119-141,227-249,349-371,386-408,415-437
Sequence
Target Sequence 
>C. ELEGANS:191851_AT
cacgtcttcgcgatcgacacaaagcatccagtggtgttctggcggattattctgatcgcc
gtgatcgccgcgccgaccattagacaattctacgtgttctcgacggatccattaactcga
cgcgttggcatgcaatcgtgggtattcgtagtgatttgtgtgctggaattggcaatttgt
gtcaagtgtggcggaataatgttcgccaagacgtccggctggaatatgttgctctgggcg
atgtctattataattggaacaatctcgtgtattctcgcatccacttggtacgccgaacac
tttggaacgaccgcccgtgtgcttgtgaagggtcgccgccgtctgtgctacctggaatcc
tcgcac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACGTCTTCGCGATCGACACAAAGC 480 199 1306 Antisense
GATTATTCTGATCGCCGTGATCGCC 663 429 1350 Antisense
AATTCTACGTGTTCTCGACGGATCC 349 177 1391 Antisense
GACGGATCCATTAACTCGACGCGTT 300 377 1407 Antisense
GACGCGTTGGCATGCAATCGTGGGT 236 381 1424 Antisense
ATAATGTTCGCCAAGACGTCCGGCT 377 25 1501 Antisense
GTCCGGCTGGAATATGTTGCTCTGG 209 477 1518 Antisense
GTTGCTCTGGGCGATGTCTATTATA 568 439 1533 Antisense
GAACAATCTCGTGTATTCTCGCATC 258 353 1562 Antisense
TACGCCGAACACTTTGGAACGACCG 376 643 1594 Antisense
TCTGTGCTACCTGGAATCCTCGCAC 252 609 1647 Antisense
Sequence Source Affymetrix Proprietary Database