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[191847_s_at]

Description E04F6.5 /REP_DB=WormBase Gene ID /WP=CE01217 /TR=Q19057 /GB=AAA68357.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Isovaleryl-CoA dehydrogenase
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 83      4grp_highest_8_8 > 28      4grp_high_8_8 > 31      6grp_highest_nonorm > 86      6grp_total > 75      4grp_total > 07      6grp_highest_sub > 04      6grp_high1_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 349
1 2 3 4 range
A 682.4
(-1.6)
P 		618.7
(-3)
P 		895.5
(3)
P 		620.2
(-3)
P 		277
B 729.2
(0.8)
P 		546.4
(-3)
P 		776.2
(1.8)
P 		833.9
(3)
P 		288
C 675.7
(-0.8)
P 		584.6
(-3)
P 		829.5
(3)
P 		719.7
(0.3)
P 		245
avr 695.8
(-0.3) 		583.2
(-3) 		833.7
(3) 		724.6
(0.4) 		251

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 759
1 2 3 4 5 6 range
A 1242.6
(3)
P 		656
(-3)
P 		857.1
(-0.9)
P 		1118.2
(1.7)
P 		945.2
(-0)
P 		1036.2
(0.9)
P 		587
B 1008.4
(1.6)
P 		624.7
(-3)
P 		926.3
(0.6)
P 		1121.3
(3)
P 		976.4
(1.2)
P 		1064.7
(2.3)
P 		497
C 917.7
(-0.9)
P 		659.7
(-3)
P 		911.2
(-0.9)
P 		1383.8
(3)
P 		737.5
(-2.4)
P 		1339.6
(2.6)
P 		724
avr 1056.2
(1.4) 		646.8
(-3) 		898.2
(-0.3) 		1207.8
(3) 		886.4
(-0.4) 		1146.8
(2.3) 		561
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 198
1 2 3 4 range
A 248.5
(3)
P 		134.3
(-1.3)
P 		234.1
(2.5)
P 		90.8
(-3)
P 		158
B 232.5
(3)
P 		134.9
(-3)
P 		179.9
(-0.2)
P 		177.7
(-0.4)
P 		98
C 223.7
(0.7)
P 		168.1
(-1.3)
P 		289.1
(3)
P 		121.4
(-3)
P 		168
avr 234.9
(3) 		145.8
(-2.1) 		234.4
(3) 		130
(-3) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 262
1 2 3 4 5 6 range
A 229.1
(-0.4)
P 		233.3
(-0.2)
P 		186.6
(-3)
P 		283.6
(2.9)
P 		285.2
(3)
P 		252.3
(1)
P 		99
B 350.9
(1.5)
P 		207.9
(-3)
P 		224.9
(-2.5)
P 		297
(-0.2)
P 		396.7
(3)
P 		397
(3)
P 		189
C 340.1
(0.4)
P 		201.3
(-3)
P 		231.9
(-2.3)
P 		278.3
(-1.1)
P 		238.7
(-2.1)
P 		448.5
(3)
P 		247
avr 306.7
(0.7) 		214.2
(-3) 		214.5
(-3) 		286.3
(-0.1) 		306.9
(0.7) 		365.9
(3) 		152
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191847_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5428
Sequence Type Exemplar sequence
Representative Public ID E04F6.5 NCBI
Target Description E04F6.5 /REP_DB=WormBase Gene ID /WP=CE01217 /TR=Q19057 /GB=AAA68357.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Isovaleryl-CoA dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026891(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026891 NCBI Caenorhabditis elegans E04F6.5a (E04F6.5) mRNA, complete cds. 11/11 None
SNAP00000032890 ENSEMBL cdna:SNAP chromosome:CEL140:II:7190232:7194694:-1 11/11 None
GENEFINDER00000032912 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7192520:7194694:-1 11/11 None
E04F6.5a ENSEMBL cdna:known chromosome:CEL140:II:7192381:7194694:-1 gene:E04F6.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001026892 3/11 Cross Hyb Matching Probes None
E04F6.5b.2 3/11 Cross Hyb Matching Probes None
E04F6.5b.1 3/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174180 2 1 NM_001026891 191847_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7192515-7194690(-) 100.0 100.0
Public Domain and Genome References
Ensembl E04F6.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA68357 Hypothetical protein E04F6.5a [Caenorhabditis elegans] ref|NP_001022062.1| E04F6.5a [Caenorhabditis elegans] 0.0
blast CAE67561 Hypothetical protein CBG13088 [Caenorhabditis briggsae] 0.0
blast AAT81203 Hypothetical protein E04F6.5b [Caenorhabditis elegans] ref|NP_001022063.1| E04F6.5b [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec ACDM_HUMAN (P11310) Acyl-CoA dehydrogenase, medium-chain specific, mitochondrial precursor (EC 1.3.99.3) (MCAD) 1.0E-132
Protein Domains
Database ID Description E-Value
Pfam IPR006090 EMBL-EBI Acyl-CoA dehydrogenase, C-terminal 1.5E-63
Pfam IPR006091 EMBL-EBI Acyl-CoA dehydrogenase, central domain 1.0E-36
Pfam IPR006092 EMBL-EBI Acyl-CoA dehydrogenase, N-terminal 3.6E-9
Sequence
Target Sequence 
>C. ELEGANS:191847_S_AT
gattcatgcgcgagactggtcttgaaagagttctccgagatcttcgtatcttcagaatct
ttgaaggagccaatgatgttctccgcctcttcattgctcttactggagcacaacatgctg
gaaagcatcttgccgagcaagcttctggagtcgggggccttattgggttggctgtttcaa
gagttaccggaggaaacactggctccaactttgggcaagtcgtcgacgcaagtcttcaag
attcagctaaagtattggatcagcaaattgctttgtttggacaaacagttcaaggattac
tcatgaagcataaaaagggaataattgatcgtcaatatgagatgcaccgtgttgccgacg
ctgccatcaacatttacagctctgccgctgttctttctcgtgccacctatgccatcaaga
acaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCATGCGCGAGACTGGTCTTGA 542 431 1238 Antisense
TTCTCCGAGATCTTCGTATCTTCAG 199 693 1268 Antisense
AAGGAGCCAATGATGTTCTCCGCCT 203 161 1301 Antisense
GCCTCTTCATTGCTCTTACTGGAGC 264 283 1322 Antisense
CTTGCCGAGCAAGCTTCTGGAGTCG 102 219 1366 Antisense
TCGGGGGCCTTATTGGGTTGGCTGT 229 245 1388 Antisense
GAAACACTGGCTCCAACTTTGGGCA 118 357 1430 Antisense
GTCGTCGACGCAAGTCTTCAAGATT 437 471 1456 Antisense
ATATGAGATGCACCGTGTTGCCGAC 54 21 1572 Antisense
TGCCATCAACATTTACAGCTCTGCC 636 585 1599 Antisense
GTGCCACCTATGCCATCAAGAACAA 10 479 1637 Antisense
Sequence Source Affymetrix Proprietary Database