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[191840_s_at] glycine C-acetyltransferase

Description T25B9.1 /REP_DB=WormBase Gene ID /WP=CE06500 /TR=Q22768 /GB=CAA94376.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=5-aminolevulinic acid syntase
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 40      4grp_highest_8_8 > 0      4grp_high_8_8 > 37      6grp_highest_nonorm > 42      6grp_total > 41      4grp_total > 42      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 684
1 2 3 4 range
A 1095.8
(-3)
P 		1779.3
(3)
P 		1315.6
(-1.1)
P 		1126
(-2.7)
P 		684
B 1100
(-3)
P 		1752.4
(3)
P 		1278.2
(-1.4)
P 		1131.9
(-2.7)
P 		652
C 1133.8
(-3)
P 		1569.5
(3)
P 		1338.1
(-0.2)
P 		1226.6
(-1.7)
P 		436
avr 1109.9
(-3) 		1700.4
(3) 		1310.6
(-1) 		1161.5
(-2.5) 		591

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1421
1 2 3 4 5 6 range
A 1120.7
(0.6)
P 		907.4
(-0.3)
P 		1691.4
(3)
P 		1052.2
(0.3)
P 		458.7
(-2.2)
P 		270.9
(-3)
P 		1421
B 926.2
(0.5)
P 		1074.1
(1.3)
P 		1373.1
(3)
P 		880
(0.3)
P 		483.3
(-1.9)
P 		292.6
(-3)
P 		1081
C 817.5
(-0.1)
P 		1035.6
(1)
P 		1409.2
(3)
P 		1194.5
(1.9)
P 		487.3
(-1.9)
P 		276.5
(-3)
P 		1133
avr 954.8
(0.3) 		1005.7
(0.6) 		1491.2
(3) 		1042.2
(0.8) 		476.4
(-2) 		280
(-3) 		1211
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 302
1 2 3 4 range
A 399
(3)
P 		386.2
(2.7)
P 		343.9
(1.6)
P 		164.8
(-3)
P 		234
B 350.8
(0.4)
P 		432.8
(3)
P 		296.2
(-1.3)
P 		241.2
(-3)
P 		192
C 375.3
(0.9)
P 		451.2
(2.6)
P 		466.4
(3)
P 		206.8
(-3)
P 		260
avr 375
(1.7) 		423.4
(3) 		368.8
(1.5) 		204.3
(-3) 		219

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 302
1 2 3 4 5 6 range
A 206.6
(-0.2)
P 		322.7
(2.1)
P 		368.4
(3)
P 		266.9
(1)
P 		138.4
(-1.6)
P 		66
(-3)
P 		302
B 322.3
(2.1)
P 		357.5
(3)
P 		333.4
(2.4)
P 		233.1
(-0)
P 		196.4
(-0.9)
P 		109.1
(-3)
P 		248
C 303
(1.7)
P 		316
(2)
P 		358.7
(3)
P 		240.2
(0.3)
P 		157.7
(-1.5)
P 		92.5
(-3)
P 		266
avr 277.3
(1.3) 		332.1
(2.5) 		353.5
(3) 		246.7
(0.6) 		164.2
(-1.3) 		89.2
(-3) 		264
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191840_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9034
Sequence Type Exemplar sequence
Representative Public ID T25B9.1 NCBI
Target Description T25B9.1 /REP_DB=WormBase Gene ID /WP=CE06500 /TR=Q22768 /GB=CAA94376.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=5-aminolevulinic acid syntase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069590(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069590 NCBI Caenorhabditis elegans T25B9.1 (T25B9.1) mRNA, complete cds. 11/11 None
SNAP00000044110 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10748384:10750074:-1 11/11 None
GENEFINDER00000044127 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10748384:10750074:-1 11/11 None
T25B9.1 ENSEMBL cdna:known chromosome:CEL140:IV:10748384:10750125:-1 gene:T25B9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10748389-10750131(-) 100.0 100.0
Public Domain and Genome References
Gene Title glycine C-acetyltransferase
Ensembl T25B9.1
Entrez Gene 177968 Entrez gene
SwissProt Q22768 EMBL-EBI
WormBase CE35627 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250837_AT aminotransferase class I and II family protein at
CANINE_2:CFAAFFX.3090.1.S1_S_AT similar to 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor (AKB ligase) (Glycine acetyltransferase) cfa
DROSOPHILA_2:1624454_AT dm
DROSGENOME1:148605_AT dm
CHICKEN:GGA.16744.1.S1_AT similar to 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor (AKB ligase) (Glycine acetyltransferase) gga
CHICKEN:GGA.8460.1.S1_S_AT similar to 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor (AKB ligase) (Glycine acetyltransferase) gga
HG-U133A:205164_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HG-U133_PLUS_2:205164_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HG-U133A_2:205164_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
U133_X3P:G7657117_3P_S_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
U133_X3P:G7657117_3P_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HG-U133A_2:36475_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HG-FOCUS:36475_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HG-U133_PLUS_2:36475_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HG-U133A:36475_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HG-U95AV2:36475_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
HU35KSUBA:AA252428_AT Glycine C-acetyltransferase (2-amino-3-ketobutyrate coenzyme A ligase) hs
MG-U74AV2:160628_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MG-U74BV2:164099_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MOE430A:1417823_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MOUSE430A_2:1417823_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MOUSE430_2:1417823_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MOE430A:1417824_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MOUSE430A_2:1417824_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MOUSE430_2:1417824_AT glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase) mm
MU19KSUBC:TC39414_AT Glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase), mRNA (cDNA clone MGC:36000 IMAGE:5125361) mm
MU19KSUBC:TC39618_AT Glycine C-acetyltransferase (2-amino-3-ketobutyrate-coenzyme A ligase), mRNA (cDNA clone MGC:36000 IMAGE:5125361) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9058 biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8483 transaminase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94376 Hypothetical protein T25B9.1 [Caenorhabditis elegans] ref|NP_501991.2| T25B9.1 [Caenorhabditis elegans] 0.0
blast CAE70862 Hypothetical protein CBG17650 [Caenorhabditis briggsae] 0.0
blast AAH64221 Hypothetical protein MGC76130 [Xenopus tropicalis] ref|NP_989284.1| hypothetical protein LOC394899 [Xenopus tropicalis] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR003408 EMBL-EBI Aminolevulinic acid synthase 3.9E-6
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 2.3E-46
Pfam IPR003408 EMBL-EBI Aminolevulinic acid synthase 3.9E-6
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 2.3E-46
Sequence
Target Sequence 
>C. ELEGANS:191840_S_AT
tattgacgagtgccatgctaccgggttctttggtaaaactggtcgaggtactgctgaagc
tgttggaggtcgtcctgatgttatcaactctacccttggaaaggctcttggtggttcaat
gggtggatatacaaccggcccaaaacccctcatcgatcttttgagacaacgctcccgtcc
ttacttgttctccaattcacttgctccaagcattgtgggaagtagcatcaaggtattcga
tctactcatgaacgattcttcattcattggctcgctccaaacaaatgtttctcacttccg
taaaagtatggctgccaatggatttactattctcggaaatgatccaactcatcctatctg
tccagttcttcttggagatgcaaagttggccgcaacaatggctgatgaacttttgaaaat
gggaatctatgtgattggtttctctttcccagttgttccaaaaggaaaagcaagaattcg
agttcaaatttcagctgctcattcgaaacaacaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATTGACGAGTGCCATGCTACCGGG 490 647 543 Antisense
AACTGGTCGAGGTACTGCTGAAGCT 602 141 579 Antisense
AGCTGTTGGAGGTCGTCCTGATGTT 86 81 600 Antisense
TGATGTTATCAACTCTACCCTTGGA 208 567 618 Antisense
AAACCCCTCATCGATCTTTTGAGAC 89 127 685 Antisense
TTACTTGTTCTCCAATTCACTTGCT 432 683 723 Antisense
TTCATTCATTGGCTCGCTCCAAACA 575 689 801 Antisense
CAAATGTTTCTCACTTCCGTAAAAG 309 193 824 Antisense
TCCTATCTGTCCAGTTCTTCTTGGA 236 593 894 Antisense
ATGTGATTGGTTTCTCTTTCCCAGT 151 49 971 Antisense
TCAGCTGCTCATTCGAAACAACACA 526 625 1033 Antisense
Sequence Source Affymetrix Proprietary Database