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[191821_at] 7TM chemoreceptor, srj family

Description Y45G12C.15 /REP_DB=WormBase Gene ID /WP=CE26103 /GEN=srj-20 /TR=Q9N4X5 /GB=AAF60588.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 91      4grp_total > 31      6grp_cgc > 97      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 36
1 2 3 4 range
A 28
(3)
A 		4
(-2.6)
A 		2.4
(-3)
A 		12
(-0.8)
A 		26
B 37.9
(3)
A 		3.2
(-3)
A 		4
(-2.9)
A 		10.1
(-1.8)
A 		35
C 7.2
(-2.5)
A 		13.7
(-0.6)
A 		5.3
(-3)
A 		26.2
(3)
A 		21
avr 24.4
(3) 		7
(-2.1) 		3.9
(-3) 		16.1
(0.6) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 19.5
(0.1)
A 		36.9
(3)
A 		9.7
(-1.6)
A 		1.3
(-3)
A 		7.4
(-2)
A 		2.1
(-2.9)
A 		36
B 37
(3)
A 		1
(-3)
A 		6.6
(-2.1)
A 		14
(-0.8)
A 		15.5
(-0.6)
A 		1.3
(-3)
A 		36
C 5.7
(-2.5)
A 		11.2
(-1.8)
A 		1.4
(-3)
A 		45.3
(2.6)
A 		48.6
(3)
A 		18.4
(-0.8)
A 		47
avr 20.7
(2) 		16.4
(0.5) 		5.9
(-3) 		20.2
(1.8) 		23.8
(3) 		7.3
(-2.5) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 10.2
(3)
A 		0.9
(-2.8)
A 		0.6
(-3)
A 		1.8
(-2.3)
A 		10
B 12.1
(3)
A 		0.8
(-3)
A 		0.9
(-2.9)
A 		2.1
(-2.3)
A 		11
C 2.4
(-1.6)
A 		3.9
(1.8)
A 		1.8
(-3)
A 		4.4
(3)
A 		3
avr 8.2
(3) 		1.9
(-2.3) 		1.1
(-3) 		2.8
(-1.6) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 3.6
(-1.5)
A 		13.1
(3)
A 		2.1
(-2.2)
A 		0.3
(-3)
A 		2.2
(-2.1)
A 		0.5
(-2.9)
A 		13
B 12.9
(3)
A 		0.3
(-3)
A 		1.6
(-2.4)
A 		3.7
(-1.4)
A 		6.3
(-0.1)
A 		0.5
(-2.9)
A 		13
C 2.1
(-2.3)
A 		3.4
(-1.8)
A 		0.4
(-3)
A 		9.1
(0.4)
A 		15.7
(3)
A 		6.2
(-0.7)
A 		15
avr 6.2
(1.3) 		5.6
(0.8) 		1.4
(-3) 		4.4
(-0.3) 		8.1
(3) 		2.4
(-2.1) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191821_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14952
Sequence Type Exemplar sequence
Representative Public ID Y45G12C.15 NCBI
Target Description Y45G12C.15 /REP_DB=WormBase Gene ID /WP=CE26103 /GEN=srj-20 /TR=Q9N4X5 /GB=AAF60588.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071303(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071303 NCBI Caenorhabditis elegans Serpentine Receptor, class J family member (srj-20) (srj-20) mRNA, complete cds. 11/11 None
SNAP00000043321 ENSEMBL cdna:SNAP chromosome:CEL140:V:2571355:2572509:-1 11/11 None
GENEFINDER00000043334 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2571355:2572588:-1 11/11 None
Y45G12C.15 ENSEMBL cdna:known chromosome:CEL140:V:2571355:2573036:-1 gene:Y45G12C.15 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2571354-2573036(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srj family
Gene Symbol srj-20
Ensembl Y45G12C.15
Entrez Gene 191940 Entrez gene
SwissProt Q9N4X5 EMBL-EBI
WormBase CE26103 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503704 Serpentine Receptor, class J family member (srj-20) [Caenorhabditis elegans] gb|AAF60588.2| Serpentine receptor, class j protein 20 [Caenorhabditis elegans] 1.0E-179
blast NP_503703 Serpentine Receptor, class J family member (srj-19) [Caenorhabditis elegans] gb|AAF60587.1| Serpentine receptor, class j protein 19 [Caenorhabditis elegans] 1.0E-147
blast NP_503704 Serpentine Receptor, class J family member (srj-20) [Caenorhabditis elegans] gb|AAF60588.2| Serpentine receptor, class j protein 20 [Caenorhabditis elegans] 1.0E-141
blast CAE62519 Hypothetical protein CBG06624 [Caenorhabditis briggsae] 1.0E-131
blast NP_503703 Serpentine Receptor, class J family member (srj-19) [Caenorhabditis elegans] gb|AAF60587.1| Serpentine receptor, class j protein 19 [Caenorhabditis elegans] 1.0E-113
Protein Domains
Database ID Description E-Value
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.9E-4
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.9E-4
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.9E-4
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503704.1 7 15-37,44-66,90-112,125-147,198-220,247-269,284-306
Y45G12C.15 7 15-37,44-66,90-112,125-147,198-220,247-269,284-306
SNAP00000043321 5 27-49,62-84,135-157,184-206,221-243
GENEFINDER00000043334 5 54-76,89-111,162-184,211-233,248-270
Sequence
Target Sequence 
>C. ELEGANS:191821_AT
atttcacatgtctgtttgggcgatgatagactatttctgtttgcactcggcttcggagat
gcgggattacattcggattccgtttagaaatctccataatgagagcatagacaatattaa
tttggtcgctggattgttttcggaagcatcgccagagattgtgcaacgatcatgggctgg
gatcgtgttgttgactgtaatagcgagctactcgatggtactgtattttgtcttgggata
taagatcataactggcctgaacatagagtcagtgacaatgagccaccaaactactcaaat
gcaaaaacaacttttcaaggcgctgaccattcagacaataattcccatttgcgtcagctt
catgccttgctcttttagtttctatggagctgctttgaatatcggatttatgaactgggt
gtactggctatctgctgtcgccgtatcaatgtttccatttttggacccattggcaataat
tctcctgctccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTCACATGTCTGTTTGGGCGATG 512 15 432 Antisense
TGCACTCGGCTTCGGAGATGCGGGA 536 577 473 Antisense
GCGGGATTACATTCGGATTCCGTTT 605 241 492 Antisense
GTTTTCGGAAGCATCGCCAGAGATT 243 449 567 Antisense
GAGATTGTGCAACGATCATGGGCTG 165 397 586 Antisense
GCGAGCTACTCGATGGTACTGTATT 69 291 634 Antisense
GTGACAATGAGCCACCAAACTACTC 596 481 703 Antisense
CTTTTCAAGGCGCTGACCATTCAGA 118 223 742 Antisense
ATAATTCCCATTTGCGTCAGCTTCA 670 25 769 Antisense
GTTTCCATTTTTGGACCCATTGGCA 419 449 882 Antisense
CATTGGCAATAATTCTCCTGCTCCC 265 217 899 Antisense
Sequence Source Affymetrix Proprietary Database