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[191819_at] Eukaryotic aspartyl proteases

Description ZK384.3 /REP_DB=WormBase Gene ID /WP=CE20430 /TR=O62509 /GB=CAB05013.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Eukaryotic aspartyl proteases
Cluster 6grp_total > 42      4grp_total > 35      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 62
1 2 3 4 range
A 34.2
(-3)
A 		68.3
(3)
A 		35
(-2.9)
A 		58.6
(1.3)
A 		34
B 42.9
(3)
A 		10.7
(-3)
A 		27.5
(0.1)
A 		21.8
(-0.9)
A 		32
C 38.9
(-3)
A 		73
(3)
A 		48.2
(-1.4)
A 		49.6
(-1.1)
A 		34
avr 38.7
(-2.2) 		50.7
(3) 		36.9
(-3) 		43.3
(-0.2) 		14

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 35.4
(-0.2)
A 		67
(2.9)
A 		68.1
(3)
A 		28.7
(-0.9)
A 		7.2
(-3)
A 		8.6
(-2.9)
A 		61
B 39.8
(-1)
A 		25.6
(-2.6)
A 		74.2
(3)
A 		40
(-0.9)
A 		39.7
(-1)
A 		22
(-3)
A 		52
C 37.8
(0.5)
A 		34.3
(0.1)
A 		62
(3)
A 		36.7
(0.3)
A 		30.2
(-0.3)
A 		5
(-3)
A 		57
avr 37.7
(-0.2) 		42.3
(0.2) 		68.1
(3) 		35.1
(-0.5) 		25.7
(-1.5) 		11.9
(-3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 12.5
(0.8)
A 		14.8
(3)
A 		9.2
(-2.4)
A 		8.6
(-3)
A 		6
B 13.7
(3)
A 		2.7
(-3)
A 		6.4
(-1)
A 		4.6
(-2)
A 		11
C 12.9
(-0.9)
A 		21
(3)
A 		16.8
(1)
A 		8.4
(-3)
A 		13
avr 13
(3) 		12.8
(2.8) 		10.8
(0.7) 		7.2
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 6.5
(-1.8)
A 		23.8
(3)
A 		14.8
(0.5)
A 		7.3
(-1.6)
A 		2.2
(-3)
A 		2.1
(-3)
A 		22
B 13.9
(0.5)
A 		8.5
(-2.8)
A 		18
(3)
A 		10.6
(-1.5)
A 		16.1
(1.8)
A 		8.2
(-3)
A 		10
C 14
(2.2)
A 		10.5
(0.7)
A 		15.8
(3)
A 		7.4
(-0.6)
A 		9.8
(0.4)
A 		1.7
(-3)
A 		14
avr 11.5
(0.7) 		14.3
(2.1) 		16.2
(3) 		8.4
(-0.8) 		9.4
(-0.3) 		4
(-3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191819_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14424
Sequence Type Exemplar sequence
Representative Public ID ZK384.3 NCBI
Target Description ZK384.3 /REP_DB=WormBase Gene ID /WP=CE20430 /TR=O62509 /GB=CAB05013.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Eukaryotic aspartyl proteases
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075252(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075252 NCBI Caenorhabditis elegans ZK384.3 (ZK384.3) mRNA, complete cds. 11/11 None
SNAP00000029483 ENSEMBL cdna:SNAP chromosome:CEL140:V:18731379:18732005:-1 10/11 None
ZK384.3 ENSEMBL cdna:known chromosome:CEL140:V:18731277:18734253:-1 gene:ZK384.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000029490 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:18731276-18734253(-) 100.0 100.0
Public Domain and Genome References
Gene Title Eukaryotic aspartyl proteases
Ensembl ZK384.3
Entrez Gene 191310 Entrez gene
SwissProt O62509 EMBL-EBI
WormBase CE20430 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4194 pepsin A activity inferred from electronic annotation QuickGO AmiGO
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05013 Hypothetical protein ZK384.3 [Caenorhabditis elegans] emb|CAA16297.1| Hypothetical protein ZK384.3 [Caenorhabditis elegans] ref|NP_507653.1| ZK384.3 [Caenorhabditis elegans] 0.0
blast T45036 hypothetical protein Y39B6B.j [imported] - Caenorhabditis elegans 1.0E-174
blast CAD31821 Hypothetical protein Y39B6A.24 [Caenorhabditis elegans] ref|NP_741673.1| Y39B6A.24 [Caenorhabditis elegans] 1.0E-127
blast CAB05013 Hypothetical protein ZK384.3 [Caenorhabditis elegans] emb|CAA16297.1| Hypothetical protein ZK384.3 [Caenorhabditis elegans] ref|NP_507653.1| ZK384.3 [Caenorhabditis elegans] 1.0E-119
blast CAD31820 Hypothetical protein Y39B6A.23 [Caenorhabditis elegans] ref|NP_741674.1| Y39B6A.23 [Caenorhabditis elegans] 1.0E-47
blast T45036 hypothetical protein Y39B6B.j [imported] - Caenorhabditis elegans 1.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 2.6E-66
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 9.5E-25
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 9.4E-24
Sequence
Target Sequence 
>C. ELEGANS:191819_AT
tcttccaggcgtctatcaactgcaagcggttcaaccaacaggcgaataggtacttcaatt
ttgatgactcgtgcattggaaacatcacgattggaactccaccacagtcagcctcggttt
tcatggacaccacttcagccaactggtgggtgattggctcaaaatgcacatctgctaact
gtaatggatactcagggatcagaaaacacaagttcaacacaacgaagtcgacgagctttg
tggaaggaaatcgaacattcagcactgaatacgggctgtgcactgggtacctgggaacgg
acacagttcagatgggaggcctcaccattacaaagcaagagcttggaattgcaaccattg
ttggtctaggttttggactgaaaccttatgtcggaattttcgagctcgcgtggccagcac
tatccgttgatcaagtaactccaccaatgcagaaactcatctcccaaaatcagctggatg
ctccgatgttcacaatctggcttgatcaaaaggatcagggcgtctatgtaggatacactg
gactgatcacctatggag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCCAGGCGTCTATCAACTGCAA 706 617 1301 Antisense
AGTCAGCCTCGGTTTTCATGGACAC 24 61 1406 Antisense
ACTGAATACGGGCTGTGCACTGGGT 631 95 1564 Antisense
GTGCACTGGGTACCTGGGAACGGAC 692 475 1578 Antisense
GGACACAGTTCAGATGGGAGGCCTC 629 525 1599 Antisense
TGGGAGGCCTCACCATTACAAAGCA 322 553 1613 Antisense
ATGTCGGAATTTTCGAGCTCGCGTG 169 45 1688 Antisense
GTAACTCCACCAATGCAGAAACTCA 162 463 1735 Antisense
CAGAAACTCATCTCCCAAAATCAGC 247 205 1750 Antisense
ATGCTCCGATGTTCACAATCTGGCT 332 43 1778 Antisense
TACACTGGACTGATCACCTATGGAG 171 641 1834 Antisense
Sequence Source Affymetrix Proprietary Database