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[191813_s_at] myosin heavy chain

Description F20G4.3 /REP_DB=WormBase Gene ID /WP=CE27133 /GEN=nmy-2 /TR=Q19658 /GB=CAA99931.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=myosin heavy chain
Cluster 6grp_highest_10_10 > 75      6grp_high1_10_10 > 57      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 86      6grp_total > 57      4grp_total > 46      6grp_highest_sub > Cu      6grp_cgc > 27      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 356
1 2 3 4 range
A 957.9
(2.6)
P 		974.4
(3)
P 		705.6
(-3)
P 		789.9
(-1.1)
P 		269
B 903.9
(3)
P 		658.6
(-2.7)
P 		658.3
(-2.7)
P 		645
(-3)
P 		259
C 884.3
(3)
P 		667
(-1.9)
P 		662.9
(-2)
P 		618.4
(-3)
P 		266
avr 915.4
(3) 		766.7
(-0.7) 		675.6
(-3) 		684.4
(-2.8) 		240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 764
1 2 3 4 5 6 range
A 1118.8
(1)
P 		1012.8
(0.1)
P 		615.1
(-3)
P 		961.7
(-0.3)
P 		884.8
(-0.9)
P 		1379.2
(3)
P 		764
B 865
(-0.9)
P 		837.9
(-1.3)
P 		860.9
(-1)
P 		918.5
(-0.1)
P 		724.6
(-3)
P 		1123.9
(3)
P 		399
C 980.4
(0.7)
P 		908.4
(-1.3)
P 		982.5
(0.7)
P 		921.6
(-1)
P 		847.4
(-3)
P 		1064.6
(3)
P 		217
avr 988.1
(-0.3) 		919.7
(-1.4) 		819.5
(-3) 		933.9
(-1.1) 		818.9
(-3) 		1189.2
(3) 		370
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 245
1 2 3 4 range
A 348.8
(3)
P 		211.5
(-0.5)
P 		184.5
(-1.2)
P 		115.6
(-3)
P 		233
B 288.2
(3)
P 		162.7
(-2)
P 		152.5
(-2.4)
P 		137.5
(-3)
P 		151
C 292.7
(3)
P 		191.8
(-0.2)
P 		231
(1)
P 		104.3
(-3)
P 		188
avr 309.9
(3) 		188.7
(-0.8) 		189.3
(-0.8) 		119.1
(-3) 		191

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 285
1 2 3 4 5 6 range
A 206.2
(-1.1)
P 		360.2
(3)
P 		134
(-3)
P 		243.9
(-0.1)
P 		267
(0.5)
P 		335.8
(2.4)
P 		226
B 301
(-0.4)
P 		278.9
(-1)
P 		209.1
(-3)
P 		243.3
(-2)
P 		294.4
(-0.6)
P 		419.1
(3)
P 		210
C 363.4
(3)
P 		277.2
(0.1)
P 		250.1
(-0.8)
P 		185.3
(-3)
P 		274.2
(-0)
P 		356.4
(2.8)
P 		178
avr 290.2
(0.2) 		305.4
(0.7) 		197.7
(-3) 		224.2
(-2.1) 		278.5
(-0.2) 		370.4
(3) 		173
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191813_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2300
Sequence Type Exemplar sequence
Representative Public ID F20G4.3 NCBI
Target Description F20G4.3 /REP_DB=WormBase Gene ID /WP=CE27133 /GEN=nmy-2 /TR=Q19658 /GB=CAA99931.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=myosin heavy chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059785(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059785 NCBI Caenorhabditis elegans Non-muscle MYosin family member (nmy-2) (nmy-2) mRNA, complete cds. 11/11 A 
F20G4.3 ENSEMBL cdna:known chromosome:CEL140:I:7934771:7942859:-1 gene:F20G4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7935127-7942600(-) 100.0 100.0
Public Domain and Genome References
Gene Title myosin heavy chain
Gene Symbol nmy-2
Ensembl F20G4.3
Entrez Gene 172562 Entrez gene
SwissProt Q19658 EMBL-EBI
WormBase CE27133 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HG-U133_PLUS_2:217545_AT myosin, heavy polypeptide 14 hs
HG-U133A_2:217545_AT myosin, heavy polypeptide 14 hs
HG-U133A:217545_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:234290_X_AT myosin, heavy polypeptide 14 hs
HG-U133B:234290_X_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:219946_X_AT myosin, heavy polypeptide 14 hs
HG-U133A:219946_X_AT myosin, heavy polypeptide 14 hs
HG-U133A_2:219946_X_AT myosin, heavy polypeptide 14 hs
HU35KSUBA:RC_AA402720_AT myosin, heavy polypeptide 14 hs
U133_X3P:G13376043_3P_A_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.287317.0.S1_3P_X_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.287317.0.S1_3P_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.250389.0.S1_3P_X_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.250389.0.S1_3P_AT myosin, heavy polypeptide 14 hs
HG-U133A_2:217660_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:217660_AT myosin, heavy polypeptide 14 hs
HG-U133A:217660_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:226988_S_AT myosin, heavy polypeptide 14 hs
HG-U133B:226988_S_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:232977_X_AT myosin, heavy polypeptide 14 hs
HG-U133B:232977_X_AT myosin, heavy polypeptide 14 hs
HG-U95C:59370_AT myosin, heavy polypeptide 14 hs
MG-U74CV2:168959_R_AT myosin, heavy polypeptide 14 mm
MOUSE430A_2:1428835_AT myosin, heavy polypeptide 14 mm
MOE430A:1428835_AT myosin, heavy polypeptide 14 mm
MOUSE430_2:1428835_AT myosin, heavy polypeptide 14 mm
MU11KSUBA:AA688923_AT myosin, heavy polypeptide 14 mm
MG-U74AV2:93481_AT myosin, heavy polypeptide 14 mm
RAE230B:1384264_AT myosin, heavy polypeptide 14 (predicted) rn
RAT230_2:1384264_AT myosin, heavy polypeptide 14 (predicted) rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
16460 myosin II inferred from sequence similarity QuickGO AmiGO
5819 spindle inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA99931 Hypothetical protein F20G4.3 [Caenorhabditis elegans] emb|CAA99841.2| Hypothetical protein F20G4.3 [Caenorhabditis elegans] ref|NP_492186.3| Non-muscle MYosin family member (nmy-2) [Caenorhabditis elegans] 0.0
blast AAC47238 non-muscle myosin heavy chain II 0.0
blast CAE63725 Hypothetical protein CBG08250 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 5.9E-14
Pfam IPR002928 EMBL-EBI Myosin tail 1.0E-126
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191813_S_AT
ggctcgtcttcgtacacaaatcaacgttgcggaagccaaagtttccagtcttgagcaaca
gctttcattggaagagcaggataagatgcgtcaaggcagaaccctgcgacgaatggaaac
gaaaatggcagagatgcagcaaatgctcgaggaagagaagagacaaggtgaaagtaatcg
tcaggctgttgatcgtcagaatgctagaatccgtcaacttcgtacacagttggaagatac
tgaggcagagcgagaccgtttgacaaacaagttgaaggacgagcggagaagagctgaaga
gatgactgatctcaatgagacactctccagagatgtttctttattgaaacagcgtgaaac
gacagctcgtagaacacctggattgattggacatcgtgaatcgagaagatttggaagcaa
caccagccttgcacgagatgaattccgtggatcagctctcacaaacgaaatgtcaccatc
tgaccgtccagcttctcgtctcacatctggaaccggatcccaactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCTCGTCTTCGTACACAAATCAAC 500 539 5511 Antisense
GAAGCCAAAGTTTCCAGTCTTGAGC 408 345 5542 Antisense
GATGCGTCAAGGCAGAACCCTGCGA 52 415 5595 Antisense
GTAATCGTCAGGCTGTTGATCGTCA 15 463 5684 Antisense
GAATCCGTCAACTTCGTACACAGTT 439 327 5717 Antisense
GAGGCAGAGCGAGACCGTTTGACAA 477 399 5752 Antisense
ACGACAGCTCGTAGAACACCTGGAT 243 93 5869 Antisense
AACACCAGCCTTGCACGAGATGAAT 254 137 5929 Antisense
TGAATTCCGTGGATCAGCTCTCACA 1 577 5949 Antisense
ACGAAATGTCACCATCTGACCGTCC 207 91 5975 Antisense
CACATCTGGAACCGGATCCCAACTT 506 193 6012 Antisense
Sequence Source Affymetrix Proprietary Database