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[191804_s_at] G-protein coupled receptor

Description T27D1.3 /REP_DB=WormBase Gene ID /WP=CE14245 /TR=SW:Q09638 /GB=CAA88290.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_high1_10_10 > 05      4grp_high_8_8 > 34      6grp_total > 05      4grp_total > 45      6grp_high1_sub > De      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 291
1 2 3 4 range
A 382.5
(3)
P 		264.3
(-0.3)
P 		172
(-2.9)
M 		166.7
(-3)
P 		216
B 377.6
(3)
P 		290.2
(0.6)
P 		155
(-3)
P 		234.2
(-0.9)
P 		223
C 427.3
(3)
P 		271.4
(-0.2)
P 		136.1
(-3)
P 		227.4
(-1.1)
P 		291
avr 395.8
(3) 		275.3
(0) 		154.4
(-3) 		209.4
(-1.6) 		241

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 320
1 2 3 4 5 6 range
A 404.3
(3)
P 		256.2
(-0.3)
P 		201.7
(-1.5)
P 		146.7
(-2.8)
P 		200.5
(-1.6)
P 		136.2
(-3)
P 		268
B 396.1
(3)
P 		295.3
(1.1)
P 		216.9
(-0.4)
P 		213.7
(-0.5)
P 		183.5
(-1.1)
P 		83.9
(-3)
P 		312
C 369.2
(3)
P 		307.6
(1.7)
P 		183.4
(-1)
M 		227.3
(-0)
P 		158.9
(-1.5)
P 		88.8
(-3)
P 		280
avr 389.9
(3) 		286.4
(0.8) 		200.7
(-1) 		195.9
(-1.1) 		181
(-1.4) 		103
(-3) 		287
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 139.3
(3)
P 		57.4
(-1.3)
P 		45
(-1.9)
M 		24.4
(-3)
P 		115
B 120.4
(3)
P 		71.7
(-0.5)
P 		35.9
(-3)
P 		49.9
(-2)
P 		85
C 141.4
(3)
P 		78
(-0.7)
P 		47.5
(-2.5)
P 		38.3
(-3)
P 		103
avr 133.7
(3) 		69
(-1) 		42.8
(-2.7) 		37.5
(-3) 		96

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 108
1 2 3 4 5 6 range
A 74.5
(1.3)
P 		91.1
(3)
P 		43.9
(-1.9)
P 		37.2
(-2.6)
P 		60.5
(-0.2)
P 		33.2
(-3)
P 		58
B 137.8
(3)
P 		98.3
(0.8)
P 		52.7
(-1.8)
P 		56.6
(-1.6)
P 		74.6
(-0.6)
P 		31.3
(-3)
P 		107
C 136.9
(3)
P 		93.9
(0.6)
P 		46.7
(-2)
M 		45.7
(-2.1)
P 		51.4
(-1.8)
P 		29.7
(-3)
P 		107
avr 116.4
(3) 		94.4
(1.4) 		47.8
(-1.8) 		46.5
(-1.9) 		62.2
(-0.8) 		31.4
(-3) 		85
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191804_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7601
Sequence Type Exemplar sequence
Representative Public ID T27D1.3 NCBI
Target Description T27D1.3 /REP_DB=WormBase Gene ID /WP=CE14245 /TR=SW:Q09638 /GB=CAA88290.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065343(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065343 NCBI Caenorhabditis elegans T27D1.3 (T27D1.3) mRNA, complete cds. 11/11 None
SNAP00000025879 ENSEMBL cdna:SNAP chromosome:CEL140:III:3683421:3685803:-1 10/11 None
GENEFINDER00000025882 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3683421:3685770:-1 11/11 None
T27D1.3 ENSEMBL cdna:known chromosome:CEL140:III:3683300:3685803:-1 gene:T27D1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3683420-3685803(-) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Ensembl T27D1.3
Entrez Gene 175470 Entrez gene
SwissProt Q09638 EMBL-EBI
WormBase CE14245 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA88290 Hypothetical protein T27D1.3 [Caenorhabditis elegans] ref|NP_497744.1| T27D1.3 [Caenorhabditis elegans] sp|Q09638|YR13_CAEEL Probable G-protein coupled receptor T27D1.3 0.0
blast CAA88290 Hypothetical protein T27D1.3 [Caenorhabditis elegans] ref|NP_497744.1| T27D1.3 [Caenorhabditis elegans] sp|Q09638|YR13_CAEEL Probable G-protein coupled receptor T27D1.3 1.0E-179
blast CAA88290 Hypothetical protein T27D1.3 [Caenorhabditis elegans] ref|NP_497744.1| T27D1.3 [Caenorhabditis elegans] sp|Q09638|YR13_CAEEL Probable G-protein coupled receptor T27D1.3 1.0E-173
blast CAE64925 Hypothetical protein CBG09746 [Caenorhabditis briggsae] 1.0E-131
blast CAE64925 Hypothetical protein CBG09746 [Caenorhabditis briggsae] 1.0E-129
blast CAB04223 Hypothetical protein F31B9.1 [Caenorhabditis elegans] ref|NP_510612.1| F31B9.1 [Caenorhabditis elegans] 1.0E-31
blast CAB04223 Hypothetical protein F31B9.1 [Caenorhabditis elegans] ref|NP_510612.1| F31B9.1 [Caenorhabditis elegans] 3.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.3E-39
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.9E-39
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.3E-39
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497744.1 7 20-42,55-77,87-109,136-158,200-219,259-278,293-315
T27D1.3 7 20-42,55-77,87-109,136-158,200-219,259-278,293-315
SNAP00000025879 7 20-42,55-77,87-109,136-158,178-200,243-262,277-299
GENEFINDER00000025882 7 10-32,44-66,76-98,125-147,189-208,248-267,282-304
Sequence
Target Sequence 
>C. ELEGANS:191804_S_AT
tgctgtgtggcattccgtcaagactaaggatagaaacactaatagtctgagattcaagct
cttccaactttcagccacctgctccaacacagttggaaaatccacttggtggcaaggata
caagctgaccgagttcctcgccttctactttgtaccatgcttcatcatcacagtagttta
cacaaaagttgcaaaatgtctgtggtgcaaggatccgacacttcaatgtgaaactcgttc
gtgcctggacaacaagtcatcatcacgaagctccgatgctctccgtactaggagaaatgt
cgtcaaaatgttgattgcatgtgtggcagtgtattttgtgtgttattcgccgattcaagt
gatattcttgtcgaaagctgtcctaaacgtgacaatccatcctccatacgacttcatcct
cctcatgaatgctcttgcaatgacttgctcggcgagcaaccctcttctttacaccctctt
ctcacaaaaattccgtagaaggttacgtgatgtcttgtactgcccgtctgacgtggaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTGTGTGGCATTCCGTCAAGACT 177 583 477 Antisense
GTCTGAGATTCAAGCTCTTCCAACT 518 469 521 Antisense
GGATACAAGCTGACCGAGTTCCTCG 484 509 592 Antisense
TCTACTTTGTACCATGCTTCATCAT 475 613 620 Antisense
GTGGTGCAAGGATCCGACACTTCAA 343 489 678 Antisense
CTCGTTCGTGCCTGGACAACAAGTC 412 233 710 Antisense
ACAAGTCATCATCACGAAGCTCCGA 592 113 728 Antisense
GTATTTTGTGTGTTATTCGCCGATT 132 453 807 Antisense
GCTCTTGCAATGACTTGCTCGGCGA 22 301 907 Antisense
GGTTACGTGATGTCTTGTACTGCCC 274 507 977 Antisense
TTGTACTGCCCGTCTGACGTGGAAA 653 707 991 Antisense
Sequence Source Affymetrix Proprietary Database