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[191801_s_at]

Description F33C8.1 /REP_DB=WormBase Gene ID /WP=CE05796 /TR=SW:Q19981 /GB=CAA93653.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=perlecan like glycoprotein
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 14      4grp_highest_8_8 > 25      4grp_high_8_8 > 60      6grp_highest_nonorm > 50      6grp_total > 05      4grp_total > 59      6grp_highest_sub > De      6grp_cgc > 41      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 188
1 2 3 4 range
A 482.5
(2.2)
P 		492.4
(2.6)
P 		501.6
(3)
P 		352.5
(-3)
P 		149
B 492.7
(3)
P 		417.9
(-0.8)
P 		443.4
(0.5)
P 		373.2
(-3)
P 		120
C 403.2
(-2.3)
P 		500.1
(1.4)
P 		540.9
(3)
P 		385.6
(-3)
P 		155
avr 459.5
(1.3) 		470.1
(1.8) 		495.3
(3) 		370.4
(-3) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 505
1 2 3 4 5 6 range
A 759.3
(3)
P 		657.4
(1.4)
P 		379.3
(-3)
P 		418.7
(-2.4)
P 		524.9
(-0.7)
P 		393
(-2.8)
P 		380
B 558.8
(3)
P 		558.9
(3)
P 		382.1
(-2.7)
P 		494.4
(0.9)
P 		373.4
(-3)
P 		421.7
(-1.4)
P 		186
C 577.6
(3)
P 		568.1
(2.8)
P 		578.7
(3)
P 		424.8
(0.2)
P 		491.3
(1.4)
P 		254
(-3)
P 		325
avr 631.9
(3) 		594.8
(2.2) 		446.7
(-1) 		446
(-1) 		463.2
(-0.7) 		356.2
(-3) 		276
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 175.7
(3)
P 		106.9
(-0.3)
P 		131.1
(0.8)
P 		51.6
(-3)
P 		124
B 157.1
(3)
P 		103.2
(-1.2)
P 		102.7
(-1.2)
P 		79.5
(-3)
P 		78
C 133.5
(0.3)
P 		143.8
(0.8)
P 		188.5
(3)
P 		65
(-3)
P 		124
avr 155.4
(3) 		118
(0.5) 		140.8
(2) 		65.4
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 151
1 2 3 4 5 6 range
A 140
(-0.7)
P 		233.8
(3)
P 		82.6
(-3)
P 		106.2
(-2.1)
P 		158.4
(0)
P 		95.7
(-2.5)
P 		151
B 194.4
(3)
P 		186
(2.5)
P 		92.8
(-3)
P 		130.9
(-0.8)
P 		151.7
(0.5)
P 		157.3
(0.8)
P 		102
C 214.1
(3)
P 		173.4
(1.1)
P 		147.3
(-0.1)
P 		85.4
(-3)
P 		159
(0.4)
P 		85
(-3)
P 		129
avr 182.8
(2) 		197.7
(3) 		107.6
(-3) 		107.5
(-3) 		156.4
(0.3) 		112.7
(-2.7) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191801_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17749
Sequence Type Exemplar sequence
Representative Public ID F33C8.1 NCBI
Target Description F33C8.1 /REP_DB=WormBase Gene ID /WP=CE05796 /TR=SW:Q19981 /GB=CAA93653.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=perlecan like glycoprotein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029454(11), NM_078042(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029454 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-53) (tag-53) mRNA, complete cds. 11/11 None
NM_078042 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-53) (tag-53) mRNA, complete cds. 11/11 None
GENEFINDER00000025096 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14710393:14718469:1 11/11 None
F33C8.1a ENSEMBL cdna:known chromosome:CEL140:X:14710393:14718689:1 gene:F33C8.1 11/11 None
F33C8.1b ENSEMBL cdna:known chromosome:CEL140:X:14710393:14718296:1 gene:F33C8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025087 7/11 Cross Hyb Matching Probes None
SNAP00000025088 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14710392-14718296(+) 95.59 100.0
Public Domain and Genome References
Ensembl F33C8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93653 Hypothetical protein F33C8.1a [Caenorhabditis elegans] ref|NP_001024625.1| Temporarily Assigned Gene name family member (tag-53) [Caenorhabditis elegans] sp|Q19981|YC81_CAEEL Hypothetical protein F33C8.1 in chromosome X precursor 0.0
blast AAK14396 attractin [Caenorhabditis elegans] 0.0
blast CAD56579 Hypothetical protein F33C8.1b [Caenorhabditis elegans] ref|NP_510443.3| Temporarily Assigned Gene name family member (tag-53) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 3.5E-4
Pfam IPR006209 EMBL-EBI EGF-like domain 4.4E-5
Pfam IPR000859 EMBL-EBI CUB 1.7E-17
Pfam IPR006652 EMBL-EBI Kelch repeat 0.0019
Pfam IPR006652 EMBL-EBI Kelch repeat 3.4E-6
Pfam IPR006652 EMBL-EBI Kelch repeat 0.0037
Pfam IPR006652 EMBL-EBI Kelch repeat 7.2E-4
Pfam IPR006652 EMBL-EBI Kelch repeat 7.0E-7
Pfam IPR006652 EMBL-EBI Kelch repeat 1.8E-5
Pfam IPR002165 EMBL-EBI Plexin 5.5E-16
Pfam IPR002165 EMBL-EBI Plexin 1.7E-7
Pfam IPR002165 EMBL-EBI Plexin 1.2E-19
Pfam IPR002165 EMBL-EBI Plexin 2.8E-15
Pfam IPR002165 EMBL-EBI Plexin 1.1E-7
Pfam IPR002165 EMBL-EBI Plexin 1.2E-19
Pfam IPR002165 EMBL-EBI Plexin 2.8E-15
Pfam IPR002165 EMBL-EBI Plexin 1.2E-19
Pfam IPR002165 EMBL-EBI Plexin 2.8E-15
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024625.1 1 1175-1197
NP_510443.3 1 1138-1160
F33C8.1a 1 1175-1197
F33C8.1b 1 1138-1160
GENEFINDER00000025096 1 1195-1217
Sequence
Target Sequence 
>C. ELEGANS:191801_S_AT
gacactgacgtcacattccaaatcagctgtgaatctccaaagggtaacgcacttgttgct
ttgaacatgacctcgtcttatgtgaacgggctcgcggataagtctcaagccatgatggtg
gatacaatttgtgactcgaaaggattccgacgtgtctatgtggcatcagataagggatat
ccatttggtccagattcgaacactacctttttcgttcgagtatacaacttcaacacgcca
gttcaaattgttgtcagttttgcacaatcaccgccgatcaactgggtcctgtttttcgtg
atatttgcagcatgcttcattgtccttctagtcgttgccggtctgctgtggatgatcaaa
gttaggatagaagcttatcgcaggaatcagcggcggatagatgaaattgagcatatggca
tcccgtccttttgcatcgacaaaaatggagctcagcatgctaagccaattctcatcggcc
ggagtccaacacctctatccatcgaaccgtgtagcaactaccgtgccggagtct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACTGACGTCACATTCCAAATCA 348 367 3313 Antisense
GGGTAACGCACTTGTTGCTTTGAAC 426 497 3354 Antisense
GACCTCGTCTTATGTGAACGGGCTC 430 283 3381 Antisense
ACGGGCTCGCGGATAAGTCTCAAGC 28 95 3398 Antisense
TTCCGACGTGTCTATGTGGCATCAG 483 699 3457 Antisense
GTCCAGATTCGAACACTACCTTTTT 158 477 3500 Antisense
CGATCAACTGGGTCCTGTTTTTCGT 265 249 3587 Antisense
TTGCCGGTCTGCTGTGGATGATCAA 217 703 3647 Antisense
CTAAGCCAATTCTCATCGGCCGGAG 506 225 3772 Antisense
CCTCTATCCATCGAACCGTGTAGCA 571 267 3804 Antisense
TGTAGCAACTACCGTGCCGGAGTCT 152 559 3822 Antisense
Sequence Source Affymetrix Proprietary Database