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[191799_at] DPY-20 protein

Description T22B3.1 /REP_DB=WormBase Gene ID /WP=CE03694 /GEN=dpy-20 /TR=SW:P34807 /GB=CAA92616.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=DPY-20 protein
Cluster 6grp_total > 22      4grp_total > 41      6grp_cgc > 33      6grp_cgc_sub > De      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 5.2
(-3)
A 		66.1
(0.7)
P 		82
(1.7)
A 		103.5
(3)
P 		98
B 9.1
(-3)
A 		135.2
(3)
P 		102
(1.4)
P 		30.2
(-2)
A 		126
C 12.7
(-3)
A 		104.9
(3)
P 		88.9
(2)
P 		83.3
(1.6)
P 		92
avr 9
(-3) 		102.1
(3) 		91
(2.3) 		72.3
(1.1) 		93

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 67.3
(1.6)
P 		49.3
(0.3)
P 		87.6
(3)
P 		6.7
(-2.7)
A 		31.9
(-0.9)
A 		1.7
(-3)
A 		86
B 47.2
(0.1)
P 		86.9
(3)
P 		50.3
(0.4)
P 		37.8
(-0.5)
A 		15.9
(-2.1)
A 		3.9
(-3)
A 		83
C 76.5
(1.5)
P 		100.2
(3)
P 		46.6
(-0.3)
A 		22.1
(-1.8)
A 		28.4
(-1.5)
A 		3.5
(-3)
A 		97
avr 63.7
(1.8) 		78.8
(3) 		61.5
(1.6) 		22.2
(-1.5) 		25.4
(-1.2) 		3
(-3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 1.9
(-3)
A 		14.4
(0.8)
P 		21.4
(3)
A 		15.1
(1.1)
P 		20
B 2.9
(-3)
A 		33.4
(3)
P 		23.6
(1.1)
P 		6.4
(-2.3)
A 		31
C 4.2
(-3)
A 		30.2
(2.8)
P 		31
(3)
P 		14
(-0.8)
P 		27
avr 3
(-3) 		26
(3) 		25.3
(2.8) 		11.8
(-0.7) 		23

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 12.4
(0.9)
P 		17.5
(2.5)
P 		19.1
(3)
P 		1.7
(-2.6)
A 		9.6
(-0)
A 		0.4
(-3)
A 		19
B 16.4
(0.3)
P 		28.9
(3)
P 		12.2
(-0.7)
P 		10
(-1.1)
A 		6.5
(-1.9)
A 		1.5
(-3)
A 		27
C 28.3
(2.5)
P 		30.6
(3)
P 		11.9
(-0.8)
A 		4.5
(-2.3)
A 		9.2
(-1.4)
A 		1.2
(-3)
A 		29
avr 19
(1.4) 		25.7
(3) 		14.4
(0.3) 		5.4
(-1.9) 		8.4
(-1.2) 		1
(-3) 		25
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191799_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8617
Sequence Type Exemplar sequence
Representative Public ID T22B3.1 NCBI
Target Description T22B3.1 /REP_DB=WormBase Gene ID /WP=CE03694 /GEN=dpy-20 /TR=SW:P34807 /GB=CAA92616.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=DPY-20 protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069814(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069814 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-20) (dpy-20) mRNA, complete cds. 11/11 None
SNAP00000034100 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11696751:11699793:-1 11/11 None
GENEFINDER00000034104 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11696751:11699793:-1 11/11 None
T22B3.1 ENSEMBL cdna:known chromosome:CEL140:IV:11697468:11699860:-1 gene:T22B3.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11697473-11699799(-) 100.0 100.0
Public Domain and Genome References
Gene Title DPY-20 protein
Gene Symbol dpy-20
Ensembl T22B3.1
Entrez Gene 178105 Entrez gene
SwissProt P34807 EMBL-EBI
WormBase CE03694 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
15986 ATP synthesis coupled proton transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16469 proton-transporting two-sector ATPase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
46933 hydrogen-transporting ATP synthase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
46961 hydrogen-transporting ATPase activity, rotational mechanism inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92616 Hypothetical protein T22B3.1 [Caenorhabditis elegans] ref|NP_502215.1| DumPY : shorter than wild-type family member (dpy-20) [Caenorhabditis elegans] sp|P34807|DPY20_CAEEL Protein dpy-20 (Dumpy-20 protein) gb|AAA85278.1| Method: conceptual translation supplied by author 1.0E-177
blast P51558 Protein dpy-20 emb|CAE62067.1| Hypothetical protein CBG06089 [Caenorhabditis briggsae] 1.0E-172
blast CAA92616 Hypothetical protein T22B3.1 [Caenorhabditis elegans] ref|NP_502215.1| DumPY : shorter than wild-type family member (dpy-20) [Caenorhabditis elegans] sp|P34807|DPY20_CAEEL Protein dpy-20 (Dumpy-20 protein) gb|AAA85278.1| Method: conceptual translation supplied by author 1.0E-171
blast S55032 dpy-20 protein - Caenorhabditis elegans 1.0E-160
blast S55032 dpy-20 protein - Caenorhabditis elegans 1.0E-154
Sequence
Target Sequence 
>C. ELEGANS:191799_AT
aaaggtagtaagatcggcgtgtgcaacaaatatgacaaaacatctcgaaagacatcacgc
agacgattatcaaaaagtgacaggacaattgaaactattcagaatgaatgatgttggaat
tcgaagtaaaatgcattatgaagtggcggacaatcctttatcaactctcccagttgttac
aaactcttacattttaccaaaaatggaaccaatggacacattcgatccctctcaatatta
tggaatgcaacaagacccaacattaaatcaacagcaatttattcaattcgagcaacctca
agcttcaattgatcctcagacatggcctctaacccatttttggcagaacactgctcaaac
aaatataatgtctcatttgggagaagcttcaacttctacattaggatcttctgtaattca
agaagctcataaaatgaatccagattcaaatgacaagagttttcaacttgatttggagca
acagaattgtgtaattattgagcaaaagttagatgataaaccacgtgtcacgaagccaac
tacaaagccgtatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAGGTAGTAAGATCGGCGTGTGCA 603 119 492 Antisense
ACATCTCGAAAGACATCACGCAGAC 689 105 531 Antisense
CGGACAATCCTTTATCAACTCTCCC 439 243 638 Antisense
AACTCTCCCAGTTGTTACAAACTCT 77 141 654 Antisense
GGAACCAATGGACACATTCGATCCC 275 529 696 Antisense
ACATTCGATCCCTCTCAATATTATG 450 105 709 Antisense
ATTTATTCAATTCGAGCAACCTCAA 354 15 768 Antisense
CAATTGATCCTCAGACATGGCCTCT 58 181 797 Antisense
AGCTTCAACTTCTACATTAGGATCT 116 79 876 Antisense
AAACCACGTGTCACGAAGCCAACTA 672 117 1009 Antisense
GAAGCCAACTACAAAGCCGTATCAA 351 345 1023 Antisense
Sequence Source Affymetrix Proprietary Database