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[191797_at]

Description ZC449.3 /REP_DB=WormBase Gene ID /WP=CE27349 /TR=Q23326 /GB=AAK39383.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=map kinase kinase; 3beta-hsd
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 32      4grp_high_8_8 > 47      6grp_highest_nonorm > 90      6grp_total > 31      4grp_total > 57      6grp_highest_sub > 02_03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 67.3
(-1.1)
A 		104.6
(3)
P 		101.8
(2.7)
P 		50
(-3)
A 		55
B 65.1
(-2)
P 		125.4
(2)
P 		140.5
(3)
P 		50.3
(-3)
A 		90
C 80.9
(-3)
P 		141.7
(2)
P 		153.2
(3)
P 		101.8
(-1.3)
P 		72
avr 71.1
(-2.7) 		123.9
(2.3) 		131.8
(3) 		67.4
(-3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 101
(0.6)
P 		138.8
(3)
P 		118.2
(1.7)
P 		110.3
(1.2)
P 		86.8
(-0.3)
P 		43.5
(-3)
P 		95
B 106.1
(0.6)
P 		104
(0.4)
P 		127
(3)
P 		105.7
(0.6)
P 		110.2
(1.1)
P 		74.6
(-3)
P 		52
C 100
(-0.7)
P 		143.9
(3)
P 		117.1
(0.8)
P 		122.5
(1.2)
P 		72.3
(-3)
P 		79.3
(-2.4)
P 		72
avr 102.4
(0.5) 		128.9
(3) 		120.8
(2.2) 		112.8
(1.5) 		89.8
(-0.7) 		65.8
(-3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 24.5
(2.3)
A 		22.7
(1.8)
P 		26.6
(3)
P 		7.3
(-3)
A 		19
B 20.8
(-0.2)
P 		31
(2.6)
P 		32.6
(3)
P 		10.7
(-3)
A 		22
C 26.8
(-1.4)
P 		40.7
(0.9)
P 		53.4
(3)
P 		17.2
(-3)
P 		36
avr 24
(-0.1) 		31.5
(1.6) 		37.5
(3) 		11.7
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 18.6
(-1.8)
P 		49.4
(3)
P 		25.8
(-0.6)
P 		28
(-0.3)
P 		26.2
(-0.6)
P 		10.6
(-3)
P 		39
B 36.9
(0.2)
P 		34.6
(-0.6)
P 		30.8
(-1.9)
P 		28
(-2.9)
P 		44.8
(3)
P 		27.8
(-3)
P 		17
C 37.1
(1)
P 		43.9
(3)
P 		29.8
(-1.1)
P 		24.6
(-2.6)
P 		23.4
(-3)
P 		26.6
(-2.1)
P 		21
avr 30.9
(-0.4) 		42.6
(3) 		28.8
(-1) 		26.9
(-1.5) 		31.5
(-0.2) 		21.7
(-3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191797_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15966
Sequence Type Exemplar sequence
Representative Public ID ZC449.3 NCBI
Target Description ZC449.3 /REP_DB=WormBase Gene ID /WP=CE27349 /TR=Q23326 /GB=AAK39383.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=map kinase kinase; 3beta-hsd
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076453(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076453 NCBI Caenorhabditis elegans ZC449.2 (ZC449.2) mRNA, complete cds. 9/11 A B 
GENEFINDER00000023565 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5021084:5024154:1 9/11 None
SNAP00000023551 ENSEMBL cdna:SNAP chromosome:CEL140:X:5022019:5022753:1 10/11 None
ZC449.2 ENSEMBL cdna:known chromosome:CEL140:X:5021182:5024203:1 gene:ZC449.2 9/11 A B 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171675 1/11 Cross Hyb Matching Probes None
NM_171676 1/11 Cross Hyb Matching Probes None
GENEFINDER00000023563 1/11 Cross Hyb Matching Probes None
SNAP00000023550 1/11 Cross Hyb Matching Probes None
ZC449.3b 1/11 Cross Hyb Matching Probes A 
ZC449.3a.1 1/11 Cross Hyb Matching Probes None
ZC449.3a.2 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5009471-5022754(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZC449.2
Entrez Gene 180774 Entrez gene
SwissProt Q23327 EMBL-EBI
WormBase CE37687 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK39384 Hypothetical protein ZC449.2 [Caenorhabditis elegans] ref|NP_508854.2| ZC449.2 [Caenorhabditis elegans] 0.0
blast T29916 hypothetical protein ZC449.2 - Caenorhabditis elegans 1.0E-128
blast CAE68681 Hypothetical protein CBG14589 [Caenorhabditis briggsae] 1.0E-108
blast T29916 hypothetical protein ZC449.2 - Caenorhabditis elegans 1.0E-102
blast CAE68681 Hypothetical protein CBG14589 [Caenorhabditis briggsae] 1.0E-102
blast T29915 hypothetical protein ZC449.3 - Caenorhabditis elegans 8.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR003014 EMBL-EBI N/apple PAN 2.0E-7
Pfam IPR003014 EMBL-EBI N/apple PAN 1.2E-10
Pfam IPR003014 EMBL-EBI N/apple PAN 2.0E-7
Sequence
Target Sequence 
>C. ELEGANS:191797_AT
atcgcaaccttcgagcaagctttgacgatctgaagaaaacaaacatctacagagactaca
tccaagaagagcatcgtgttactgatggttgtgtttctgtggtcttccatagaaagttca
gtacctgccagctttatgggcacgatggaactcacaacggcgctgaggtggtgtatttgg
aggatcacgacttttacattcgcagcagttgggatggacagtgccaagacaaaatcgctc
cgattcgtggatacaagcaaaatattcagcaactgcagactccacaaataagcacgcttc
caaaagctcaaactattccaaatttaccaaagttcgagaattctgatttcccaaaagacc
cgaaccctcaagtcataagagataatgttcgaaaagctccagttcattttgaaaattcaa
ttgtacaagaaatagagcgccaggcgatggaagacgaatttgatatggactatttaacta
caacatcaccaccaaagaaaagaacacatccagatatcattacctcgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGCAACCTTCGAGCAAGCTTTGA 619 35 3104 Antisense
TGATGGTTGTGTTTCTGTGGTCTTC 273 567 3186 Antisense
GTTCAGTACCTGCCAGCTTTATGGG 531 443 3219 Antisense
AACGGCGCTGAGGTGGTGTATTTGG 635 143 3259 Antisense
GGATCACGACTTTTACATTCGCAGC 297 511 3285 Antisense
AGTGCCAAGACAAAATCGCTCCGAT 649 59 3323 Antisense
ATATTCAGCAACTGCAGACTCCACA 548 19 3365 Antisense
AGACTCCACAAATAAGCACGCTTCC 28 71 3380 Antisense
AGACCCGAACCCTCAAGTCATAAGA 291 69 3459 Antisense
CTATTTAACTACAACATCACCACCA 162 223 3573 Antisense
ACATCCAGATATCATTACCTCGTTC 375 107 3609 Antisense
Sequence Source Affymetrix Proprietary Database