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[191789_at] dihydroorotate dehydrogenase

Description W02D3.2 /REP_DB=WormBase Gene ID /WP=CE14420 /TR=O01815 /GB=AAK21481.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=dihydroorotate dehydrogenase
Cluster 6grp_highest_10_10 > 15      6grp_high1_10_10 > 20      4grp_highest_8_8 > 26      4grp_high_8_8 > 60      6grp_highest_nonorm > 52      6grp_total > 50      4grp_total > 59      6grp_highest_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 431
1 2 3 4 range
A 649
(0.2)
P 		675
(0.7)
P 		793.6
(3)
P 		483.9
(-3)
P 		310
B 688.3
(1.4)
P 		678.8
(1.3)
P 		778.7
(3)
P 		429.8
(-3)
P 		349
C 684.1
(0.3)
P 		691.9
(0.4)
P 		860.7
(3)
P 		471
(-3)
P 		390
avr 673.8
(0.6) 		681.9
(0.8) 		811
(3) 		461.6
(-3) 		349

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 406
1 2 3 4 5 6 range
A 640.3
(-0.4)
P 		623.1
(-0.7)
P 		792.6
(3)
P 		643.3
(-0.3)
P 		531.9
(-2.7)
P 		520.4
(-3)
P 		272
B 666.5
(2.2)
P 		639
(1.3)
P 		691
(3)
P 		671.5
(2.4)
P 		506.3
(-3)
P 		617.8
(0.6)
P 		185
C 555.3
(-0.1)
P 		632.2
(1.3)
P 		605.3
(0.8)
P 		733
(3)
P 		386.6
(-3)
P 		600
(0.7)
P 		346
avr 620.7
(1) 		631.4
(1.2) 		696.3
(3) 		682.6
(2.6) 		474.9
(-3) 		579.4
(-0.2) 		221
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 229
1 2 3 4 range
A 236.3
(3)
P 		146.5
(-0.3)
P 		207.5
(2)
P 		70.8
(-3)
P 		166
B 219.5
(3)
P 		167.6
(0.6)
P 		180.4
(1.2)
P 		91.6
(-3)
P 		128
C 226.5
(1)
P 		198.9
(0.3)
P 		300
(3)
P 		79.4
(-3)
P 		221
avr 227.4
(2.9) 		171
(0.6) 		229.3
(3) 		80.6
(-3) 		149

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 118
(-3)
P 		221.6
(3)
P 		172.6
(0.2)
P 		163.2
(-0.4)
P 		160.5
(-0.5)
P 		126.7
(-2.5)
P 		104
B 231.9
(3)
P 		212.7
(1.2)
P 		167.8
(-3)
P 		177.9
(-2.1)
P 		205.7
(0.5)
P 		230.4
(2.9)
P 		64
C 205.8
(3)
P 		192.9
(2)
P 		154
(-0.9)
P 		147.4
(-1.3)
P 		125.1
(-3)
P 		200.8
(2.6)
P 		81
avr 185.2
(-0.1) 		209.1
(3) 		164.8
(-2.7) 		162.8
(-3) 		163.8
(-2.9) 		186
(0) 		46
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191789_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1598
Sequence Type Exemplar sequence
Representative Public ID W02D3.2 NCBI
Target Description W02D3.2 /REP_DB=WormBase Gene ID /WP=CE14420 /TR=O01815 /GB=AAK21481.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=dihydroorotate dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059529(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059529 NCBI Caenorhabditis elegans W02D3.2 (W02D3.2) mRNA, complete cds. 11/11 None
GENEFINDER00000042734 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6744825:6749145:1 11/11 None
SNAP00000042720 ENSEMBL cdna:SNAP chromosome:CEL140:I:6745667:6749145:1 11/11 None
W02D3.2 ENSEMBL cdna:known chromosome:CEL140:I:6744820:6749295:1 gene:W02D3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6745595-6749074(+) 100.0 100.0
Public Domain and Genome References
Gene Title dihydroorotate dehydrogenase
Ensembl W02D3.2
Entrez Gene 172392 Entrez gene
SwissProt O01815 EMBL-EBI
WormBase CE14420 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:17827_AT dihydroorotate dehydrogenase, mitochondrial / dihydroorotate oxidase / DHOdehase (PYRD) at
ATH1-121501:249830_AT dihydroorotate dehydrogenase, mitochondrial / dihydroorotate oxidase / DHOdehase (PYRD) at
CANINE_2:CFA.17204.1.S1_AT similar to dihydroorotate dehydrogenase isoform 1 precursor cfa
CANINE_2:CFAAFFX.30969.1.S1_AT similar to dihydroorotate dehydrogenase isoform 1 precursor cfa
DROSOPHILA_2:1623792_A_AT Dihydroorotate dehydrogenase dm
DROSGENOME1:142757_AT Dihydroorotate dehydrogenase dm
CHICKEN:GGA.4705.2.S1_AT dihydroorotate dehydrogenase gga
CHICKEN:GGA.4705.2.S1_S_AT dihydroorotate dehydrogenase gga
CHICKEN:GGAAFFX.13107.1.S1_S_AT dihydroorotate dehydrogenase gga
HG-U95AV2:33195_AT dihydroorotate dehydrogenase hs
HU35KSUBA:M94065_S_AT dihydroorotate dehydrogenase hs
HU35KSUBA:HUM_ALU_AT dihydroorotate dehydrogenase hs
HU35KSUBB:HUM_ALU_AT dihydroorotate dehydrogenase hs
HU35KSUBC:HUM_ALU_AT dihydroorotate dehydrogenase hs
HU35KSUBD:HUM_ALU_AT dihydroorotate dehydrogenase hs
HUGENEFL:HUM_ALU_AT dihydroorotate dehydrogenase hs
HC-G110:HUM_ALU_AT dihydroorotate dehydrogenase hs
HUGENEFL:M94065_AT dihydroorotate dehydrogenase hs
U133_X3P:HS.94925.0.S1_3P_AT dihydroorotate dehydrogenase hs
HG-U133A_2:213632_AT dihydroorotate dehydrogenase hs
HG-U133_PLUS_2:213632_AT dihydroorotate dehydrogenase hs
HG-U133A:213632_AT dihydroorotate dehydrogenase hs
HG-U95C:49842_AT Dihydroorotate dehydrogenase hs
HG-U95C:65061_AT Dihydroorotate dehydrogenase hs
MOE430A:1417581_AT dihydroorotate dehydrogenase mm
MOUSE430A_2:1417581_AT dihydroorotate dehydrogenase mm
MOUSE430_2:1417581_AT dihydroorotate dehydrogenase mm
MU11KSUBA:AF029667_AT dihydroorotate dehydrogenase mm
MG-U74AV2:103683_AT dihydroorotate dehydrogenase mm
MG-U74AV2:161397_R_AT dihydroorotate dehydrogenase mm
MG-U74CV2:171571_F_AT dihydroorotate dehydrogenase mm
MOE430A:1417582_S_AT dihydroorotate dehydrogenase mm
MOUSE430A_2:1417582_S_AT dihydroorotate dehydrogenase mm
MOUSE430_2:1417582_S_AT dihydroorotate dehydrogenase mm
MU19KSUBC:TC40989_AT Dihydroorotate dehydrogenase, mRNA (cDNA clone MGC:35899 IMAGE:5253723) mm
RG-U34A:X80778CDS_AT dihydroorotate dehydrogenase rn
RG-U34C:RC_AI230216_AT dihydroorotate dehydrogenase rn
RAE230A:1382348_AT dihydroorotate dehydrogenase rn
RAT230_2:1382348_AT dihydroorotate dehydrogenase rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6207 'de novo' pyrimidine base biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4152 dihydroorotate dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
4158 dihydroorotate oxidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK21481 Hypothetical protein W02D3.2 [Caenorhabditis elegans] ref|NP_491930.1| W02D3.2 [Caenorhabditis elegans] 0.0
blast CAE60439 Hypothetical protein CBG04047 [Caenorhabditis briggsae] 0.0
blast AAH86819 Zgc:103492 [Danio rerio] ref|NP_001008608.1| hypothetical protein LOC494065 [Danio rerio] 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR001295 EMBL-EBI Dihydroorotate dehydrogenase 1.0E-126
Pfam IPR001295 EMBL-EBI Dihydroorotate dehydrogenase 1.5E-126
Pfam IPR001295 EMBL-EBI Dihydroorotate dehydrogenase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191789_AT
attggtgtcaactactttgctccacattgtgattatctggttctcaatgtatcatcacct
aacactcctgggctcagaagtatgcaaaagaaaagtgatttggagaagctgttggcctat
gtacaccaagcgcttgaaatgcacaagctggaaaagcagcctcaagtttttctgaaaatt
gctccggatctaattgaatcagaattaaaagacattgcacaagttgtgacgaataaaaag
tttgcgatcgatggaattattgtatcgaataccacaattgcacgacccgactatctcaga
tcagagaataaaacggaaacaggaggattgagcggagcgccggttagagaaattagtacg
gaatgtgtcagaaaaatatacaaactcacaaacggacaaattccaattatcggctgtgga
ggagtgttcagtggagcagatgcatatgaaaagattagagccggtgctagtctggtccaa
ctctattccgcgttcgtctatgaaggatttccggtaatcggaaaaatcaaacgagaactt
gtcgaacttttacggaaagatggattttcgcatgtttcggaagcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTGGTGTCAACTACTTTGCTCCAC 236 5 631 Antisense
GTGATTATCTGGTTCTCAATGTATC 88 485 659 Antisense
AATGTATCATCACCTAACACTCCTG 191 165 676 Antisense
AACACTCCTGGGCTCAGAAGTATGC 613 127 691 Antisense
TGGCCTATGTACACCAAGCGCTTGA 565 543 743 Antisense
AAAAGCAGCCTCAAGTTTTTCTGAA 105 131 782 Antisense
TCGAATACCACAATTGCACGACCCG 324 599 895 Antisense
GGATTGAGCGGAGCGCCGGTTAGAG 507 503 955 Antisense
GTGCTAGTCTGGTCCAACTCTATTC 632 479 1094 Antisense
TCTATTCCGCGTTCGTCTATGAAGG 154 615 1112 Antisense
GGATTTTCGCATGTTTCGGAAGCAA 449 503 1192 Antisense
Sequence Source Affymetrix Proprietary Database