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[191780_s_at] ubiquitin conjugating enzyme

Description F40G9.3 /REP_DB=WormBase Gene ID /WP=CE19850 /TR=Q9TZ69 /GB=AAC68796.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ubiquitin conjugating enzyme
Cluster 6grp_highest_10_10 > 92      6grp_high1_10_10 > 49      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 77      6grp_total > 39      4grp_total > 58      6grp_highest_sub > Cu      6grp_cgc > 08      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 883
1 2 3 4 range
A 1562.2
(-1.2)
P 		1988.1
(2.8)
P 		2011.3
(3)
P 		1375
(-3)
P 		636
B 1554.7
(-1.8)
P 		1644.4
(-0.9)
P 		2017.6
(3)
P 		1440
(-3)
P 		578
C 1618.1
(0.4)
P 		1992.7
(3)
P 		1525.6
(-0.3)
P 		1135.1
(-3)
P 		858
avr 1578.3
(-0.2) 		1875.1
(3) 		1851.5
(2.7) 		1316.7
(-3) 		558

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1006
1 2 3 4 5 6 range
A 1159.6
(-0.2)
P 		1574.6
(3)
P 		794.2
(-3)
P 		1493.2
(2.3)
P 		1130.9
(-0.4)
P 		1579.4
(3)
P 		785
B 1124.4
(0.9)
P 		932.1
(-3)
P 		934.5
(-3)
P 		971.5
(-2.2)
P 		1228.3
(3)
P 		1094.9
(0.3)
P 		296
C 1800.3
(3)
P 		869.7
(-3)
P 		1609.9
(1.8)
P 		1098.9
(-1.5)
P 		1705.9
(2.4)
P 		1171.4
(-1.1)
P 		931
avr 1361.4
(3) 		1125.5
(-2.7) 		1112.9
(-3) 		1187.9
(-1.2) 		1355
(2.8) 		1281.9
(1.1) 		249
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 382
1 2 3 4 range
A 568.8
(3)
P 		431.6
(0.8)
P 		525.8
(2.3)
P 		201.3
(-3)
P 		368
B 495.8
(3)
P 		406.1
(0.2)
P 		467.5
(2.1)
P 		306.9
(-3)
P 		189
C 535.6
(2.4)
P 		572.9
(3)
P 		531.7
(2.4)
P 		191.4
(-3)
P 		382
avr 533.4
(3) 		470.2
(1.7) 		508.3
(2.5) 		233.2
(-3) 		300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 494
1 2 3 4 5 6 range
A 213.8
(-2.4)
P 		560
(3)
P 		173
(-3)
P 		378.7
(0.2)
P 		341.3
(-0.4)
P 		384.5
(0.3)
P 		387
B 391.2
(0.6)
P 		310.3
(-1.2)
P 		226.9
(-3)
P 		257.3
(-2.3)
P 		499
(3)
P 		408.3
(1)
P 		272
C 667.3
(3)
P 		265.4
(-2.4)
P 		409.7
(-0.5)
P 		221
(-3)
P 		552
(1.4)
P 		392.1
(-0.7)
P 		446
avr 424.1
(1.8) 		378.6
(0.4) 		269.9
(-3) 		285.7
(-2.5) 		464.1
(3) 		395
(0.9) 		194
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191780_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8317
Sequence Type Exemplar sequence
Representative Public ID F40G9.3 NCBI
Target Description F40G9.3 /REP_DB=WormBase Gene ID /WP=CE19850 /TR=Q9TZ69 /GB=AAC68796.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=ubiquitin conjugating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064773(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064773 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-20) (ubc-20) mRNA, complete cds. 11/11 None
SNAP00000021775 ENSEMBL cdna:SNAP chromosome:CEL140:III:188840:190574:1 11/11 None
GENEFINDER00000021780 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:188840:190574:1 10/11 None
F40G9.3.2 ENSEMBL cdna:known chromosome:CEL140:III:188832:190874:1 gene:F40G9.3 11/11 None
F40G9.3.1 ENSEMBL cdna:known chromosome:CEL140:III:188835:190944:1 gene:F40G9.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:188839-190574(+) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin conjugating enzyme
Gene Symbol ubc-20
Ensembl F40G9.3
Entrez Gene 175185 Entrez gene
SwissProt Q9TZ69 EMBL-EBI
WormBase CE19850 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:248478_AT ubiquitin-conjugating enzyme, putative at
CANINE:1590490_S_AT similar to huntingtin interacting protein 2 cfa
CANINE_2:CFAAFFX.24469.1.S1_S_AT similar to huntingtin interacting protein 2 cfa
DROSOPHILA_2:1628595_AT Ubiquitin conjugating enzyme 4 dm
DROSGENOME1:154399_AT Ubiquitin conjugating enzyme 4 dm
HG-U133A:202346_AT huntingtin interacting protein 2 hs
HG-U133_PLUS_2:202346_AT huntingtin interacting protein 2 hs
HG-FOCUS:202346_AT huntingtin interacting protein 2 hs
HG-U133A_2:202346_AT huntingtin interacting protein 2 hs
HG-U133_PLUS_2:202347_S_AT huntingtin interacting protein 2 hs
HG-U133A_2:202347_S_AT huntingtin interacting protein 2 hs
HG-U133A:202347_S_AT huntingtin interacting protein 2 hs
HU35KSUBA:U58522_AT huntingtin interacting protein 2 hs
HUGENEFL:U58522_AT huntingtin interacting protein 2 hs
HU35KSUBD:RC_H78385_S_AT huntingtin interacting protein 2 hs
U133_X3P:G4996607_3P_A_AT huntingtin interacting protein 2 hs
U133_X3P:G12545382_3P_AT huntingtin interacting protein 2 hs
HG-U95AV2:40121_AT huntingtin interacting protein 2 hs
HU35KSUBA:RC_AA121121_AT Huntingtin interacting protein 2 hs
HU35KSUBA:C02374_S_AT Huntingtin interacting protein 2 hs
U133_X3P:HS.7822.0.S1_3P_AT Huntingtin interacting protein 2 hs
HG-U133_PLUS_2:225179_AT Huntingtin interacting protein 2 hs
HG-U133B:225179_AT Huntingtin interacting protein 2 hs
HG-U133_PLUS_2:244537_AT Huntingtin interacting protein 2 hs
HG-U133B:244537_AT Huntingtin interacting protein 2 hs
HG-U95D:78298_AT Huntingtin interacting protein 2 hs
HU35KSUBB:RC_H53038_AT Huntingtin interacting protein 2 hs
HU35KSUBC:RC_N73604_AT Huntingtin interacting protein 2 hs
HU35KSUBB:RC_T91015_AT Huntingtin interacting protein 2 hs
U133_X3P:HS.132879.0.A1_3P_AT Huntingtin interacting protein 2 hs
MG-U74AV2:92547_AT huntingtin interacting protein 2 mm
MG-U74AV2:92548_G_AT huntingtin interacting protein 2 mm
MG-U74BV2:115706_AT huntingtin interacting protein 2 mm
MG-U74BV2:108321_AT huntingtin interacting protein 2 mm
MG-U74CV2:166746_AT huntingtin interacting protein 2 mm
MOE430A:1417186_AT huntingtin interacting protein 2 mm
MOUSE430A_2:1417186_AT huntingtin interacting protein 2 mm
MOUSE430_2:1417186_AT huntingtin interacting protein 2 mm
MOE430A:1417188_S_AT huntingtin interacting protein 2 mm
MOUSE430A_2:1417188_S_AT huntingtin interacting protein 2 mm
MOUSE430_2:1417188_S_AT huntingtin interacting protein 2 mm
MOE430A:1417187_AT huntingtin interacting protein 2 mm
MOUSE430A_2:1417187_AT huntingtin interacting protein 2 mm
MOUSE430_2:1417187_AT huntingtin interacting protein 2 mm
MOE430B:1443148_AT huntingtin interacting protein 2 mm
MOUSE430_2:1443148_AT huntingtin interacting protein 2 mm
MG-U74CV2:137559_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
MOUSE430_2:1441012_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
MOE430B:1441012_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
MOE430B:1442671_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
MOUSE430_2:1442671_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
MOE430B:1444212_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
MOUSE430_2:1444212_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
MU19KSUBC:TC40038_AT Huntingtin interacting protein 2, mRNA (cDNA clone MGC:5809 IMAGE:3496393) mm
RG-U34C:RC_AI179857_AT huntingtin interacting protein 2 (predicted) rn
RAE230A:1373063_AT huntingtin interacting protein 2 (predicted) rn
RAT230_2:1373063_AT huntingtin interacting protein 2 (predicted) rn
RAE230B:1385259_AT huntingtin interacting protein 2 (predicted) rn
RAT230_2:1385259_AT huntingtin interacting protein 2 (predicted) rn
YEAST_2:1776783_AT Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC68796 Ubiquitin conjugating enzyme protein 20 [Caenorhabditis elegans] ref|NP_497174.1| UBiquitin Conjugating enzyme family member (ubc-20) [Caenorhabditis elegans] 1.0E-112
blast AAC68796 Ubiquitin conjugating enzyme protein 20 [Caenorhabditis elegans] ref|NP_497174.1| UBiquitin Conjugating enzyme family member (ubc-20) [Caenorhabditis elegans] 1.0E-108
blast CAE69517 Hypothetical protein CBG15726 [Caenorhabditis briggsae] 1.0E-104
blast AAY52456 ubiquitin conjugating enzyme E2 [Caenorhabditis remanei] 1.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 3.0E-69
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 4.4E-67
Sequence
Target Sequence 
>C. ELEGANS:191780_S_AT
gacactccatacgccggtggaatgttcgatttggatattaagattccagaccaatatcca
ttctcgccaccaaatgtcaagttcagcacaaaaatctggcacccgaatgtcagctcgcag
accggcgtcatttgcctcgatattcttaaggatcaatgggccgcttcgctgactcttcgc
acggttctcctgtcgattcaggcgttgatgtgcacaccggagcccaaggatcctcaagat
gccgtcgtcgccaagcaatacatggagaagccagcagttttcaaggccaccgccgagtac
tggacgacaaaattcgcaaagggagccgtgaaaaacgccggataccaggctctcgtcgac
aagctcaaggatatgggagtccgtgaggaagaggctgtcagcgtgctcagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACTCCATACGCCGGTGGAATGT 353 367 154 Antisense
ATTCCAGACCAATATCCATTCTCGC 352 7 196 Antisense
TTCTCGCCACCAAATGTCAAGTTCA 29 693 214 Antisense
AAATCTGGCACCCGAATGTCAGCTC 214 117 245 Antisense
CGGCGTCATTTGCCTCGATATTCTT 105 243 276 Antisense
GTTCTCCTGTCGATTCAGGCGTTGA 256 443 337 Antisense
AGGCGTTGATGTGCACACCGGAGCC 24 55 353 Antisense
GCCCAAGGATCCTCAAGATGCCGTC 479 285 375 Antisense
AAGCCAGCAGTTTTCAAGGCCACCG 124 149 421 Antisense
AAAAACGCCGGATACCAGGCTCTCG 664 127 484 Antisense
GGAAGAGGCTGTCAGCGTGCTCAGC 438 531 540 Antisense
Sequence Source Affymetrix Proprietary Database