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[191765_at]

Description F52E1.4 /REP_DB=WormBase Gene ID /WP=CE04632 /GEN=gcy-7 /TR=Q20661 /GB=AAB37038.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster 6grp_total > 16      4grp_total > 46      6grp_cgc > 13      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 121
1 2 3 4 range
A 128.3
(3)
P 		65.2
(-0.7)
P 		26
(-3)
A 		28.6
(-2.8)
A 		102
B 99.6
(3)
P 		47.2
(-0.4)
A 		7.1
(-3)
A 		30.2
(-1.5)
A 		93
C 89.5
(3)
P 		43.2
(-0.9)
A 		18
(-3)
A 		42.2
(-1)
A 		72
avr 105.8
(3) 		51.9
(-0.6) 		17
(-3) 		33.7
(-1.9) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 61
1 2 3 4 5 6 range
A 63
(3)
M 		32.9
(0)
A 		19.5
(-1.3)
A 		15
(-1.7)
A 		12.7
(-1.9)
A 		1.8
(-3)
A 		61
B 37.8
(3)
A 		21.4
(0.1)
P 		4.1
(-3)
A 		10.9
(-1.8)
A 		12.1
(-1.6)
A 		23
(0.4)
A 		34
C 48.6
(3)
P 		33.9
(0.7)
A 		19.9
(-1.4)
A 		16.8
(-1.9)
A 		9.8
(-3)
A 		21.5
(-1.2)
A 		39
avr 49.8
(3) 		29.4
(-0.2) 		14.5
(-2.5) 		14.2
(-2.6) 		11.5
(-3) 		15.4
(-2.4) 		38
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 46.7
(3)
P 		14.2
(-1.6)
P 		6.8
(-2.6)
A 		4.2
(-3)
A 		43
B 31.8
(3)
P 		11.7
(-1)
A 		1.7
(-3)
A 		6.4
(-2.1)
A 		30
C 29.6
(3)
P 		12.4
(-1.4)
A 		6.3
(-3)
A 		7.1
(-2.8)
A 		23
avr 36
(3) 		12.8
(-1.5) 		4.9
(-3) 		5.9
(-2.8) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 11.6
(2.9)
M 		11.7
(3)
A 		4.2
(-1)
A 		3.8
(-1.2)
A 		3.8
(-1.2)
A 		0.4
(-3)
A 		11
B 13.2
(3)
A 		7.1
(0)
P 		1
(-3)
A 		2.9
(-2.1)
A 		4.9
(-1.1)
A 		8.6
(0.7)
A 		12
C 18
(3)
P 		10.4
(-0.1)
A 		5.1
(-2.2)
A 		3.4
(-2.9)
A 		3.2
(-3)
A 		7.2
(-1.4)
A 		15
avr 14.3
(3) 		9.7
(0.5) 		3.4
(-3) 		3.4
(-3) 		4
(-2.7) 		5.4
(-1.9) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191765_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11742
Sequence Type Exemplar sequence
Representative Public ID F52E1.4 NCBI
Target Description F52E1.4 /REP_DB=WormBase Gene ID /WP=CE04632 /GEN=gcy-7 /TR=Q20661 /GB=AAB37038.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=guanylate cyclase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028784(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028784 NCBI Caenorhabditis elegans Guanylyl CYclase family member (gcy-7) (gcy-7) mRNA, complete cds. 10/11 None
SNAP00000034422 ENSEMBL cdna:SNAP chromosome:CEL140:V:8389946:8394914:1 10/11 None
GENEFINDER00000034441 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8389946:8394898:1 10/11 None
F52E1.4a ENSEMBL cdna:known chromosome:CEL140:V:8389946:8395087:1 gene:F52E1.4 10/11 None
F52E1.4b ENSEMBL cdna:known chromosome:CEL140:V:8389946:8394914:1 gene:F52E1.4 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001028783 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179222 2 1 NM_001028784 191765_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8389946-8394915(+) 100.0 100.0
Public Domain and Genome References
Ensembl F52E1.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAQ62451 Guanylyl cyclase protein 7, isoform b [Caenorhabditis elegans] ref|NP_001023955.1| Guanylyl CYclase family member (gcy-7) [Caenorhabditis elegans] 0.0
blast A89130 protein F52E1.4 [imported] - Caenorhabditis elegans 0.0
blast AAQ62450 Guanylyl cyclase protein 7, isoform a [Caenorhabditis elegans] ref|NP_001023954.1| Guanylyl CYclase family member (gcy-7) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-12
Pfam IPR001054 EMBL-EBI Guanylate cyclase 1.1E-71
Pfam IPR001828 EMBL-EBI Extracellular ligand-binding receptor 1.6E-34
Pfam IPR001054 EMBL-EBI Guanylate cyclase 6.9E-90
Pfam IPR000719 EMBL-EBI Protein kinase 3.7E-12
Pfam IPR001054 EMBL-EBI Guanylate cyclase 6.9E-90
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023955.1 2 490-512,656-678
F52E1.4a 2 490-512,656-678
F52E1.4b 2 490-512,656-678
SNAP00000034422 2 492-514,658-680
GENEFINDER00000034441 2 490-512,656-678
Sequence
Target Sequence 
>C. ELEGANS:191765_AT
gatcggagacggatatctttgtgtatcagggttacctcacagaaatggcaatgagcatgt
cagacagattgcgttaatgtctcttgcatttttatcaagcctccagttctttagagttcc
acatttaccaagtgaacggattaacttgagaattggaatgaattgtgggtcggttgtagc
tggagttgttggattaacaatgccacgtttttgtctttttggagatgctgttaacactgc
aagtcggatggagagtaatggaaaacctggaaaaattcacgtatccgctgaagccaatag
aatgcttcatctagtaggaggatttgataccgagtcacgaggagaagttataattaaagg
aaaaggggtaatggaaacattttggcttacggggcaaggaactggagcagtctcaggagc
ccgtcatgtatcggcgaagaaggtttcaaaaaaggtttgtttggatattcagatcactta
tttttgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCGGAGACGGATATCTTTGTGTA 532 417 2838 Antisense
GGGTTACCTCACAGAAATGGCAATG 185 321 2866 Antisense
GATTGCGTTAATGTCTCTTGCATTT 16 435 2904 Antisense
TTTATCAAGCCTCCAGTTCTTTAGA 81 675 2928 Antisense
GTTCTTTAGAGTTCCACATTTACCA 87 437 2943 Antisense
ATTGTGGGTCGGTTGTAGCTGGAGT 190 3 2999 Antisense
TGCCACGTTTTTGTCTTTTTGGAGA 214 587 3038 Antisense
GATGCTGTTAACACTGCAAGTCGGA 105 413 3061 Antisense
AATTCACGTATCCGCTGAAGCCAAT 122 177 3111 Antisense
GGAGGATTTGATACCGAGTCACGAG 57 517 3154 Antisense
GATATTCAGATCACTTATTTTTGTT 447 427 3301 Antisense
Sequence Source Affymetrix Proprietary Database