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[191760_at] NADH-cytochrome B5 reductase

Description T05H4.4 /REP_DB=WormBase Gene ID /WP=CE13275 /TR=O16522 /GB=AAB66010.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=NADH-cytochrome B5 reductase
Cluster 6grp_total > 27      4grp_total > 22      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 180
1 2 3 4 range
A 223.4
(2.8)
P 		101.8
(-3)
A 		119.9
(-2.1)
A 		227.1
(3)
P 		125
B 253.6
(2.2)
P 		109.3
(-3)
A 		121.8
(-2.6)
A 		276
(3)
P 		167
C 247.4
(3)
P 		143.1
(-1.1)
A 		96.2
(-3)
P 		223.9
(2.1)
P 		151
avr 241.5
(3) 		118.1
(-2.7) 		112.6
(-3) 		242.3
(3) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 143.5
(3)
P 		52.9
(-3)
A 		53.5
(-3)
P 		64.7
(-2.2)
A 		94.6
(-0.2)
A 		91.1
(-0.5)
A 		91
B 105
(1.3)
P 		126.5
(3)
M 		50.1
(-3)
A 		54.2
(-2.7)
A 		83.8
(-0.4)
A 		98.4
(0.8)
P 		76
C 131.4
(3)
M 		79.6
(-0.8)
P 		110.6
(1.5)
P 		58.5
(-2.3)
A 		49.6
(-3)
A 		90.1
(-0)
P 		82
avr 126.6
(3) 		86.3
(-0.6) 		71.4
(-1.9) 		59.1
(-3) 		76
(-1.5) 		93.2
(0) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 81.3
(3)
P 		22.1
(-3)
A 		31.4
(-2.1)
A 		33.2
(-1.9)
P 		59
B 80.9
(3)
P 		27
(-3)
A 		28.2
(-2.9)
A 		58.8
(0.5)
P 		54
C 81.9
(3)
P 		41.2
(-2)
A 		33.5
(-3)
P 		37.8
(-2.5)
P 		48
avr 81.4
(3) 		30.1
(-3) 		31
(-2.9) 		43.3
(-1.5) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 26.5
(2.3)
P 		18.8
(-0.5)
A 		11.7
(-3)
P 		16.4
(-1.3)
A 		28.5
(3)
A 		22.2
(0.8)
A 		17
B 36.5
(1.9)
P 		42.1
(3)
M 		12.2
(-3)
A 		14.4
(-2.6)
A 		34
(1.4)
A 		36.7
(1.9)
P 		30
C 48.7
(3)
M 		24.3
(-1)
P 		28.1
(-0.3)
P 		11.8
(-3)
A 		16.1
(-2.3)
A 		30.2
(-0)
P 		37
avr 37.2
(3) 		28.4
(0.7) 		17.3
(-2.2) 		14.2
(-3) 		26.2
(0.1) 		29.7
(1) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191760_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11681
Sequence Type Exemplar sequence
Representative Public ID T05H4.4 NCBI
Target Description T05H4.4 /REP_DB=WormBase Gene ID /WP=CE13275 /TR=O16522 /GB=AAB66010.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=NADH-cytochrome B5 reductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072238(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072238 NCBI Caenorhabditis elegans T05H4.4 (T05H4.4) mRNA, complete cds. 11/11 None
SNAP00000040310 ENSEMBL cdna:SNAP chromosome:CEL140:V:6441530:6442630:1 11/11 None
GENEFINDER00000040321 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6441530:6442630:1 11/11 None
T05H4.4 ENSEMBL cdna:known chromosome:CEL140:V:6441530:6442630:1 gene:T05H4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6441529-6442630(+) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-cytochrome B5 reductase
Ensembl T05H4.4
Entrez Gene 188150 Entrez gene
SwissProt O16522 EMBL-EBI
WormBase CE13275 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605653_AT similar to NADH-cytochrome b5 reductase (B5R) (Diaphorase 1) cfa
CANINE:1596918_S_AT similar to NADH-cytochrome b5 reductase (B5R) (Diaphorase 1) cfa
CANINE_2:CFA.15074.1.S1_AT similar to NADH-cytochrome b5 reductase (B5R) (Diaphorase 1) cfa
CANINE_2:CFAAFFX.2338.1.S1_S_AT similar to NADH-cytochrome b5 reductase (B5R) (Diaphorase 1) cfa
CHICKEN:GGAAFFX.11758.1.S1_S_AT similar to cytochrome b-5 reductase gga
CHICKEN:GGAAFFX.8957.1.S1_AT similar to cytochrome b-5 reductase gga
HG-U133_PLUS_2:201885_S_AT cytochrome b5 reductase 3 hs
HG-U133A:201885_S_AT cytochrome b5 reductase 3 hs
HG-FOCUS:201885_S_AT cytochrome b5 reductase 3 hs
HG-U133A_2:201885_S_AT cytochrome b5 reductase 3 hs
HU35KSUBD:RC_AA453429_S_AT cytochrome b5 reductase 3 hs
HUGENEFL:M28713_AT cytochrome b5 reductase 3 hs
U133_X3P:G6552326_3P_A_AT cytochrome b5 reductase 3 hs
U133_X3P:HS2.274464.3.S1_3P_X_AT cytochrome b5 reductase 3 hs
U133_X3P:HS2.274464.3.S1_3P_S_AT cytochrome b5 reductase 3 hs
U133_X3P:HS2.274464.3.S1_3P_AT cytochrome b5 reductase 3 hs
U133_X3P:1554574_3P_A_AT cytochrome b5 reductase 3 hs
HG-U133_PLUS_2:1554574_A_AT cytochrome b5 reductase 3 hs
HG-U95AV2:36668_AT cytochrome b5 reductase 3 hs
MOUSE430_2:1430734_AT cytochrome b5 reductase 3 mm
MOE430A:1430734_AT cytochrome b5 reductase 3 mm
MOUSE430A_2:1430734_AT cytochrome b5 reductase 3 mm
MOUSE430A_2:1422185_A_AT cytochrome b5 reductase 3 mm
MOUSE430_2:1422185_A_AT cytochrome b5 reductase 3 mm
MOE430A:1422185_A_AT cytochrome b5 reductase 3 mm
MOUSE430_2:1422186_S_AT cytochrome b5 reductase 3 mm
MOE430A:1422186_S_AT cytochrome b5 reductase 3 mm
MOUSE430A_2:1422186_S_AT cytochrome b5 reductase 3 mm
MG-U74AV2:94284_AT cytochrome b5 reductase 3 mm
MG-U74CV2:165973_R_AT cytochrome b5 reductase 3 mm
MOE430A:1425329_A_AT cytochrome b5 reductase 3 mm
MOUSE430A_2:1425329_A_AT cytochrome b5 reductase 3 mm
MOUSE430_2:1425329_A_AT cytochrome b5 reductase 3 mm
MU19KSUBA:TC23428_S_AT Diaphorase 1 (NADH), mRNA (cDNA clone MGC:36220 IMAGE:4238275) mm
MU19KSUBB:TC31014_AT Diaphorase 1 (NADH), mRNA (cDNA clone MGC:36220 IMAGE:4238275) mm
MU19KSUBB:TC31014_G_AT Diaphorase 1 (NADH), mRNA (cDNA clone MGC:36220 IMAGE:4238275) mm
MU19KSUBB:TC31015_S_AT Diaphorase 1 (NADH), mRNA (cDNA clone MGC:36220 IMAGE:4238275) mm
RAE230A:1370808_AT diaphorase 1 rn
RAT230_2:1370808_AT diaphorase 1 rn
RT-U34:RC_AA963839_S_AT diaphorase 1 rn
RG-U34A:RC_AA963839_S_AT diaphorase 1 rn
RT-U34:J03867_S_AT diaphorase 1 rn
RG-U34A:J03867_S_AT diaphorase 1 rn
RT-U34:D00636POLY_A_SITE#1_S_AT diaphorase 1 rn
RG-U34A:D00636POLY_A_SITE#1_S_AT diaphorase 1 rn
RT-U34:RC_AI229440_S_AT diaphorase 1 rn
RG-U34A:RC_AI229440_S_AT diaphorase 1 rn
RG-U34A:D00636CDS_S_AT diaphorase 1 rn
RT-U34:X77117EXON#1-3_AT Diaphorase 1 rn
RG-U34A:X77117EXON#1-3_AT Diaphorase 1 rn
RT-U34:X65190MRNA_S_AT Diaphorase 1 rn
RG-U34A:X65190MRNA_S_AT Diaphorase 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_504639 T05H4.4 [Caenorhabditis elegans] gb|AAB66010.1| Hypothetical protein T05H4.4 [Caenorhabditis elegans] 1.0E-175
blast NP_504639 T05H4.4 [Caenorhabditis elegans] gb|AAB66010.1| Hypothetical protein T05H4.4 [Caenorhabditis elegans] 1.0E-161
blast NP_504638 T05H4.5 [Caenorhabditis elegans] gb|AAB66011.1| Hypothetical protein T05H4.5 [Caenorhabditis elegans] 1.0E-132
blast CAE71880 Hypothetical protein CBG18935 [Caenorhabditis briggsae] 1.0E-130
Protein Domains
Database ID Description E-Value
Pfam IPR008333 EMBL-EBI Oxidoreductase FAD-binding region 2.6E-55
Pfam IPR001433 EMBL-EBI Oxidoreductase FAD/NAD(P)-binding 8.4E-44
Pfam IPR008333 EMBL-EBI Oxidoreductase FAD-binding region 2.6E-55
Pfam IPR001433 EMBL-EBI Oxidoreductase FAD/NAD(P)-binding 8.4E-44
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504639.1 2 6-25,173-195
T05H4.4 2 6-25,173-195
GENEFINDER00000040321 2 6-25,173-195
Sequence
Target Sequence 
>C. ELEGANS:191760_AT
ctataccccagtgtcttgtgacttagacttgggatatgtagatttgatggttaaagtgta
ttttaagaatacacatgaaagattcccggatggtgggaaaatgagccaacatttggagag
cctcaagattggagatactgttagcttccgtggtccacatggaagcataatttataaggg
aagtggtctattcactgtacgaatggataagaaggccgagccaaagaatcgatttttcaa
gcatctttccatgattgctggaggtaccggaatcacaccgatgcttcaagtcattgccgc
cattcttcgtgatccaattgacgctactcagatcagacttctgtttgcaaatcagaccga
agacgatattctgtgcaggaaagagctcgacgagctagccgagaagcatccaactcgttt
ccgtgtatggtatactgtaagcaaagcgtcaaaagattggagatattctacagggcatat
aaatgaggaaatgatcaaggaacatctattcccttccaatgaggaatctgctgtgctgtt
gtgtggaccaccagcaatgatcaactgtgcgtgcattccaaatcttgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTATACCCCAGTGTCTTGTGACTTA 591 221 297 Antisense
GGAGATACTGTTAGCTTCCGTGGTC 33 519 427 Antisense
CTGGAGGTACCGGAATCACACCGAT 520 241 554 Antisense
TTCGTGATCCAATTGACGCTACTCA 299 693 602 Antisense
GACGCTACTCAGATCAGACTTCTGT 430 379 616 Antisense
CTGTGCAGGAAAGAGCTCGACGAGC 444 239 667 Antisense
GAGCTAGCCGAGAAGCATCCAACTC 2 389 688 Antisense
GAACATCTATTCCCTTCCAATGAGG 118 351 796 Antisense
GAGGAATCTGCTGTGCTGTTGTGTG 542 405 817 Antisense
GCTGTTGTGTGGACCACCAGCAATG 510 295 831 Antisense
TGTGCGTGCATTCCAAATCTTGATA 590 555 862 Antisense
Sequence Source Affymetrix Proprietary Database