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[191752_at] folate transporter protein

Description C06H2.4 /REP_DB=WormBase Gene ID /WP=CE05236 /TR=SW:Q17766 /GB=CAA99770.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=folate transporter protein
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 21      4grp_high_8_8 > 41      6grp_highest_nonorm > 61      6grp_total > 41      4grp_total > 54      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 481
1 2 3 4 range
A 488.6
(3)
P 		249.6
(-0.4)
P 		247.9
(-0.4)
P 		64.9
(-3)
A 		424
B 505.2
(3)
P 		306.4
(-0.2)
P 		271.1
(-0.8)
P 		132.2
(-3)
M 		373
C 546
(3)
P 		301.7
(-0.7)
P 		246.3
(-1.6)
P 		153.3
(-3)
P 		393
avr 513.3
(3) 		285.9
(-0.4) 		255.1
(-0.9) 		116.8
(-3) 		397

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 379
1 2 3 4 5 6 range
A 482.5
(2.3)
P 		435.1
(1.6)
P 		523.7
(3)
P 		434.3
(1.5)
P 		217.1
(-2)
P 		154.3
(-3)
P 		369
B 427.9
(2.1)
P 		442.4
(2.4)
P 		476.1
(3)
P 		373.4
(1.1)
P 		225.5
(-1.6)
P 		152.7
(-3)
P 		323
C 370.3
(1)
P 		419.2
(1.9)
P 		479
(3)
P 		465.7
(2.8)
P 		191.3
(-2.2)
P 		144.7
(-3)
P 		334
avr 426.9
(1.8) 		432.2
(1.9) 		492.9
(3) 		424.5
(1.8) 		211.3
(-1.9) 		150.6
(-3) 		342
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 177.9
(3)
P 		54.2
(-1.4)
P 		64.8
(-1)
P 		9.5
(-3)
A 		168
B 161.1
(3)
P 		75.7
(-0.9)
P 		62.8
(-1.4)
P 		28.2
(-3)
M 		133
C 180.8
(3)
P 		86.7
(-0.6)
P 		85.9
(-0.7)
P 		25.8
(-3)
P 		155
avr 173.3
(3) 		72.2
(-1) 		71.2
(-1) 		21.2
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 117
1 2 3 4 5 6 range
A 88.9
(-0.4)
P 		154.7
(3)
P 		114.1
(0.9)
P 		110.2
(0.7)
P 		65.5
(-1.6)
P 		37.6
(-3)
P 		117
B 148.9
(3)
P 		147.2
(2.9)
P 		115.6
(0.8)
P 		98.9
(-0.3)
P 		91.6
(-0.7)
P 		57
(-3)
P 		92
C 137.3
(3)
P 		127.9
(2.4)
P 		121.9
(2)
P 		93.6
(0.1)
P 		61.9
(-2.1)
P 		48.4
(-3)
P 		89
avr 125
(1.9) 		143.3
(3) 		117.2
(1.4) 		100.9
(0.3) 		73
(-1.4) 		47.7
(-3) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191752_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13336
Sequence Type Exemplar sequence
Representative Public ID C06H2.4 NCBI
Target Description C06H2.4 /REP_DB=WormBase Gene ID /WP=CE05236 /TR=SW:Q17766 /GB=CAA99770.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=folate transporter protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073432(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073432 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-330) (tag-330) mRNA, complete cds. 11/11 None
SNAP00000033420 ENSEMBL cdna:SNAP chromosome:CEL140:V:11144086:11145651:1 11/11 None
GENEFINDER00000033422 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11144086:11145651:1 11/11 None
C06H2.4 ENSEMBL cdna:known chromosome:CEL140:V:11144086:11145454:1 gene:C06H2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11144085-11145454(+) 100.0 100.0
Public Domain and Genome References
Gene Title folate transporter protein
Gene Symbol tag-330
Ensembl C06H2.4
Entrez Gene 179544 Entrez gene
SwissProt Q17766 EMBL-EBI
WormBase CE05236 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23422.1.S1_S_AT similar to Thiamine transporter 1 (THTR-1) (ThTr1) (Thiamine carrier 1) (TC1) (Solute carrier family 19, member 2) cfa
CANINE_2:CFAAFFX.23425.1.S1_AT similar to Thiamine transporter 1 (THTR-1) (ThTr1) (Thiamine carrier 1) (TC1) (Solute carrier family 19, member 2) cfa
CANINE_2:CFAAFFX.23425.1.S1_S_AT similar to Thiamine transporter 1 (THTR-1) (ThTr1) (Thiamine carrier 1) (TC1) (Solute carrier family 19, member 2) cfa
DROSOPHILA_2:1628221_AT dm
DROSGENOME1:142727_AT dm
HG-U133_PLUS_2:209681_AT solute carrier family 19 (thiamine transporter), member 2 hs
HG-U133A:209681_AT solute carrier family 19 (thiamine transporter), member 2 hs
HG-U133A_2:209681_AT solute carrier family 19 (thiamine transporter), member 2 hs
HU35KSUBB:RC_N30856_AT solute carrier family 19 (thiamine transporter), member 2 hs
U133_X3P:G5453325_3P_AT solute carrier family 19 (thiamine transporter), member 2 hs
HG-U95AV2:35248_AT solute carrier family 19 (thiamine transporter), member 2 hs
U133_X3P:HS2.303324.1.S1_3P_S_AT Solute carrier family 19 (thiamine transporter), member 2 hs
HG-U133_PLUS_2:1556602_AT Solute carrier family 19 (thiamine transporter), member 2 hs
MOE430A:1417902_AT solute carrier family 19 (thiamine transporter), member 2 mm
MOUSE430A_2:1417902_AT solute carrier family 19 (thiamine transporter), member 2 mm
MOUSE430_2:1417902_AT solute carrier family 19 (thiamine transporter), member 2 mm
MOUSE430_2:1426117_A_AT solute carrier family 19 (thiamine transporter), member 2 mm
MOUSE430A_2:1426117_A_AT solute carrier family 19 (thiamine transporter), member 2 mm
MOE430A:1426117_A_AT solute carrier family 19 (thiamine transporter), member 2 mm
MOE430B:1441315_S_AT solute carrier family 19 (thiamine transporter), member 2 mm
MOUSE430_2:1441315_S_AT solute carrier family 19 (thiamine transporter), member 2 mm
MG-U74CV2:166751_AT solute carrier family 19 (thiamine transporter), member 2 mm
RG-U34C:RC_AI233639_AT solute carrier family 19 (thiamine transporter), member 2 (predicted) rn
RAE230B:1390863_AT solute carrier family 19 (thiamine transporter), member 2 (predicted) rn
RAT230_2:1390863_AT solute carrier family 19 (thiamine transporter), member 2 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5542 folic acid binding inferred from electronic annotation QuickGO AmiGO
8518 reduced folate carrier activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA99770 Hypothetical protein C06H2.4 [Caenorhabditis elegans] ref|NP_505833.1| Temporarily Assigned Gene name family member (tag-330) [Caenorhabditis elegans] sp|Q17766|FOLT1_CAEEL Folate-like transporter 1.0E-178
blast CAA99770 Hypothetical protein C06H2.4 [Caenorhabditis elegans] ref|NP_505833.1| Temporarily Assigned Gene name family member (tag-330) [Caenorhabditis elegans] sp|Q17766|FOLT1_CAEEL Folate-like transporter 1.0E-166
blast CAE75176 Hypothetical protein CBG23116 [Caenorhabditis briggsae] 1.0E-152
blast CAE75176 Hypothetical protein CBG23116 [Caenorhabditis briggsae] 1.0E-148
blast AAM47550 thiamine transporter variant b [Mus musculus] gb|AAL85635.1| high-affinity thiamine transporter splice variant [Mus musculus] 1.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR002666 EMBL-EBI Reduced folate carrier 1.0E-126
Pfam IPR002666 EMBL-EBI Reduced folate carrier 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505833.1 7 46-68,73-95,102-121,131-153,160-182,279-301,322-344
C06H2.4 7 46-68,73-95,102-121,131-153,160-182,279-301,322-344
SNAP00000033420 9 46-68,73-95,133-152,162-184,279-301,306-325,332-351,366-388,401-423
GENEFINDER00000033422 9 46-68,73-95,133-152,162-184,279-301,306-325,332-351,366-388,401-423
Sequence
Target Sequence 
>C. ELEGANS:191752_AT
ggttcttaatcaaatctctctgggagcagtctgtcttgtcacaatcattgcgatatttct
gccacgtgtcaagtcggagaaagccaaagtttcaatgagagcacacgagattgttgagca
gcaaactgttgaatctgtggaaagcgtacaaacgccaaaagcagtaaaaatgagctacac
ccgcgaatatttcaagaaaatatcagaagagcttcaaatctgttcaaaaaatcaagaact
gttgaaatggtcattgtggtgggctcttgcaagttgtggagtatatcaagtacaaaacta
tacccaatcactttggaaagaattgcaaaataatcctgatgacgttgcaaatggagttgt
tgaatttgtgaacactgctctcggagcctttctttcattattcattcatcatttgtcaat
cgactggacactaagatatgttttaagagcaaatgtagcaaaagaactatcttcaaataa
tcatggtctcatattcgggtgcaatacatttgtcgcagtttgtcttcaatcacttctcac
actggttgtagtagattcgcggttcttac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTCTTAATCAAATCTCTCTGGGA 510 507 495 Antisense
GAGCAGTCTGTCTTGTCACAATCAT 357 389 518 Antisense
ATTTCTGCCACGTGTCAAGTCGGAG 573 15 549 Antisense
TGAGCTACACCCGCGAATATTTCAA 53 569 665 Antisense
AAATGGTCATTGTGGTGGGCTCTTG 163 121 739 Antisense
GAATTTGTGAACACTGCTCTCGGAG 384 325 856 Antisense
GGAGCCTTTCTTTCATTATTCATTC 41 523 877 Antisense
ATCATGGTCTCATATTCGGGTGCAA 420 29 974 Antisense
GTCGCAGTTTGTCTTCAATCACTTC 563 473 1006 Antisense
CAATCACTTCTCACACTGGTTGTAG 220 179 1021 Antisense
GTTGTAGTAGATTCGCGGTTCTTAC 348 415 1039 Antisense
Sequence Source Affymetrix Proprietary Database