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[191751_at] ammonium transporter like

Description F49E11.3 /REP_DB=WormBase Gene ID /WP=CE05899 /TR=SW:Q20605 /GB=CAA94345.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=ammonium transporter like
Cluster 6grp_total > 34      4grp_total > 21      6grp_cgc > 21      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 76.8
(0.6)
P 		66.7
(0.1)
A 		13.1
(-3)
A 		117.9
(3)
P 		105
B 91.2
(3)
P 		42.4
(-1)
A 		18.4
(-3)
A 		88.1
(2.7)
P 		73
C 74.3
(-0.1)
P 		51.1
(-1.9)
A 		36.8
(-3)
A 		115.5
(3)
P 		79
avr 80.8
(1.1) 		53.4
(-0.8) 		22.8
(-3) 		107.2
(3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 74.7
(3)
A 		34.3
(-2.3)
M 		29.2
(-3)
A 		35.7
(-2.1)
A 		43.4
(-1.1)
A 		55.2
(0.4)
A 		46
B 53.3
(3)
P 		51.7
(2.8)
P 		48.8
(2.4)
A 		39.7
(1.3)
A 		35.4
(0.7)
A 		6.6
(-3)
A 		47
C 37.1
(-0.4)
A 		48.4
(0.9)
P 		67.6
(3)
A 		13.2
(-3)
A 		26.4
(-1.5)
A 		36.4
(-0.4)
A 		54
avr 55
(3) 		44.8
(0.6) 		48.5
(1.5) 		29.5
(-3) 		35.1
(-1.7) 		32.7
(-2.2) 		26
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 28
(3)
P 		14.5
(-0.3)
A 		3.4
(-3)
A 		17.3
(0.4)
P 		25
B 29.1
(3)
P 		10.5
(-1.5)
A 		4.3
(-3)
A 		18.8
(0.5)
P 		25
C 24.6
(3)
P 		14.7
(-2)
A 		12.8
(-3)
A 		19.5
(0.4)
P 		12
avr 27.2
(3) 		13.2
(-1.1) 		6.8
(-3) 		18.5
(0.4) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 13.8
(3)
A 		12.2
(1.7)
M 		6.4
(-3)
A 		9.1
(-0.8)
A 		13.1
(2.4)
A 		13.4
(2.7)
A 		7
B 18.5
(3)
P 		17.2
(2.5)
P 		11.8
(0.5)
A 		10.5
(0)
A 		14.4
(1.5)
A 		2.5
(-3)
A 		16
C 13.7
(1.6)
A 		14.8
(2)
P 		17.2
(3)
A 		2.7
(-3)
A 		8.5
(-0.6)
A 		12.2
(0.9)
A 		15
avr 15.3
(3) 		14.7
(2.5) 		11.8
(0.3) 		7.4
(-3) 		12
(0.5) 		9.4
(-1.5) 		8
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191751_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8903
Sequence Type Exemplar sequence
Representative Public ID F49E11.3 NCBI
Target Description F49E11.3 /REP_DB=WormBase Gene ID /WP=CE05899 /TR=SW:Q20605 /GB=CAA94345.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=ammonium transporter like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070095(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070095 NCBI Caenorhabditis elegans AMmonium Transporter homolog family member (amt-2) (amt-2) mRNA, complete cds. 11/11 None
SNAP00000011568 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13043591:13046828:1 11/11 None
GENEFINDER00000011585 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13044434:13046828:1 11/11 None
F49E11.3 ENSEMBL cdna:known chromosome:CEL140:IV:13042382:13046828:1 gene:F49E11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13042387-13046834(+) 99.52 100.0
Public Domain and Genome References
Gene Title ammonium transporter like
Gene Symbol amt-2
Ensembl F49E11.3
Entrez Gene 186047 Entrez gene
SwissProt Q20605 EMBL-EBI
WormBase CE05899 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8519 ammonium transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94345 Hypothetical protein F49E11.3 [Caenorhabditis elegans] ref|NP_502496.1| AMmonium Transporter homolog family member (amt-2) [Caenorhabditis elegans] sp|Q20605|AMT2_CAEEL Putative ammonium transporter 0.0
blast CAE56330 Hypothetical protein CBG23995 [Caenorhabditis briggsae] 0.0
blast CAE56330 Hypothetical protein CBG23995 [Caenorhabditis briggsae] 1.0E-172
blast CAE56330 Hypothetical protein CBG23995 [Caenorhabditis briggsae] 1.0E-170
blast CAE57701 Hypothetical protein CBG00705 [Caenorhabditis briggsae] 1.0E-110
Protein Domains
Database ID Description E-Value
Pfam IPR010256 EMBL-EBI Rh-like protein/ammonium transporter 1.0E-126
Pfam IPR010256 EMBL-EBI Rh-like protein/ammonium transporter 1.0E-126
Pfam IPR010256 EMBL-EBI Rh-like protein/ammonium transporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502496.1 8 35-57,72-91,173-191,206-226,247-269,351-373,385-407,442-464
F49E11.3 8 35-57,72-91,173-191,206-226,247-269,351-373,385-407,442-464
SNAP00000011568 7 47-69,84-106,126-148,158-180,192-214,239-261,282-316
GENEFINDER00000011585 6 92-110,125-145,166-188,270-292,304-326,361-383
Sequence
Target Sequence 
>C. ELEGANS:191751_AT
ttgtactttacaccctggcaagcaacattagttggagcaattggatcgtctcttgctctt
gcagcatatccagtgacggaatggcttaagattgacgacccagttggtgtatttccagtg
catgtagttggatccatatggggtatgattgctcctgcaatctttgtctatcgaagaccc
atgaattttggaccgcccgagtgtgattttcagacatctgacgagatcaacggactgctc
tatggcggtggcttctaccttctatttcttcaatctttcgtgatattggtaattggaact
tatagtgctatttgtgcatttatcatactgtttctgattcatcattctccagttggtttg
cgtgttgacaagtataccgaagagcttggagcagatctcattgagcacggtttggctgga
tttaatgtgatgacttatacaattgagaagaaactcgatacaaaaactctatccgctgtc
cttatgataattgttagatggagagccaaagcaaaacttggagctcagagaaggaaaaag
attcatgatagtggatcagttgcaccgcagcaagctga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTACTTTACACCCTGGCAAGCAA 235 705 1063 Antisense
CTCTTGCAGCATATCCAGTGACGGA 499 227 1118 Antisense
GACGACCCAGTTGGTGTATTTCCAG 655 377 1156 Antisense
ATATGGGGTATGATTGCTCCTGCAA 545 19 1198 Antisense
GCTCCTGCAATCTTTGTCTATCGAA 356 299 1213 Antisense
AGACCCATGAATTTTGGACCGCCCG 309 1 1237 Antisense
CGAGATCAACGGACTGCTCTATGGC 408 249 1284 Antisense
ACCTTCTATTTCTTCAATCTTTCGT 469 87 1319 Antisense
GATTCATCATTCTCCAGTTGGTTTG 614 429 1398 Antisense
ATACAAAAACTCTATCCGCTGTCCT 107 25 1520 Antisense
GATCAGTTGCACCGCAGCAAGCTGA 667 419 1616 Antisense
Sequence Source Affymetrix Proprietary Database