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[191750_at] homeobox protein

Description C27A12.5 /REP_DB=WormBase Gene ID /WP=CE20530 /GEN=ceh-2 /TR=O01962 /GB=AAB93639.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=homeobox protein
Cluster 6grp_total > 34      4grp_total > 30      6grp_cgc > 24      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 121.8
(2.9)
P 		59.2
(-3)
P 		58.9
(-3)
A 		122.8
(3)
P 		64
B 170.7
(3)
P 		66.6
(-1.5)
P 		31.4
(-3)
A 		62.5
(-1.7)
P 		139
C 131.5
(3)
P 		103.7
(1.3)
P 		32.3
(-3)
A 		115.6
(2)
P 		99
avr 141.3
(3) 		76.5
(-0.9) 		40.9
(-3) 		100.3
(0.5) 		100

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 133.5
(3)
P 		80.5
(0.2)
P 		78.3
(0.1)
P 		34.7
(-2.3)
A 		21.1
(-3)
A 		37.3
(-2.1)
A 		112
B 88.2
(3)
P 		66
(0.8)
P 		75.5
(1.7)
P 		27.9
(-3)
A 		33.9
(-2.4)
A 		54.4
(-0.4)
P 		60
C 73.5
(3)
P 		71.5
(2.7)
P 		56
(0.1)
A 		49.1
(-1)
A 		37.2
(-3)
P 		54.8
(-0.1)
P 		36
avr 98.4
(3) 		72.7
(0.7) 		69.9
(0.5) 		37.2
(-2.4) 		30.7
(-3) 		48.8
(-1.4) 		68
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 44.3
(3)
P 		12.9
(-3)
P 		15.4
(-2.5)
A 		18
(-2)
P 		31
B 54.4
(3)
P 		16.4
(-1.8)
P 		7.3
(-3)
A 		13.3
(-2.2)
P 		47
C 43.5
(3)
P 		29.8
(0.4)
P 		11.3
(-3)
A 		19.5
(-1.5)
P 		32
avr 47.4
(3) 		19.7
(-1.6) 		11.3
(-3) 		16.9
(-2.1) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 24.6
(1.9)
P 		28.6
(3)
P 		17
(-0.1)
P 		8.8
(-2.4)
A 		6.4
(-3)
A 		9.1
(-2.3)
A 		22
B 30.7
(3)
P 		22
(0.8)
P 		18.3
(-0.2)
P 		7.4
(-3)
A 		13.8
(-1.4)
A 		20.3
(0.3)
P 		23
C 27.2
(3)
P 		21.8
(1.1)
P 		14.3
(-1.5)
A 		9.9
(-3)
A 		12
(-2.3)
P 		18.3
(-0.1)
P 		17
avr 27.5
(3) 		24.1
(1.9) 		16.5
(-0.5) 		8.7
(-3) 		10.7
(-2.4) 		15.9
(-0.7) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191750_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2174
Sequence Type Exemplar sequence
Representative Public ID C27A12.5 NCBI
Target Description C27A12.5 /REP_DB=WormBase Gene ID /WP=CE20530 /GEN=ceh-2 /TR=O01962 /GB=AAB93639.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=homeobox protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059345(11), AY246428(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059345 NCBI Caenorhabditis elegans C.Elegans Homeobox family member (ceh-2) (ceh-2) mRNA, complete cds. 11/11 None
AY246428 NCBI Caenorhabditis elegans clone 4E homeodomain protein CEH-2 (ceh-2) mRNA, complete cds. 11/11 None
GENEFINDER00000016786 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6055085:6058522:-1 11/11 None
SNAP00000016770 ENSEMBL cdna:SNAP chromosome:CEL140:I:6057404:6058522:-1 10/11 None
C27A12.5 ENSEMBL cdna:known chromosome:CEL140:I:6054915:6058572:-1 gene:C27A12.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6055084-6058522(-) 100.0 100.0
Public Domain and Genome References
Gene Title homeobox protein
Gene Symbol ceh-2
Ensembl C27A12.5
Entrez Gene 191615 Entrez gene
SwissProt O01962 EMBL-EBI
WormBase CE20530 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB93639 C.elegans homeobox protein 2 [Caenorhabditis elegans] ref|NP_491746.1| C.Elegans Homeobox family member (ceh-2) [Caenorhabditis elegans] gb|AAO91836.1| homeodomain protein CEH-2 [Caenorhabditis elegans] gb|AAO91835.1| homeodomain protein CEH-2 [Caenorhabditis elegans] 2.0E-86
blast CAE72921 Hypothetical protein CBG20241 [Caenorhabditis briggsae] 1.0E-76
blast AAB93639 C.elegans homeobox protein 2 [Caenorhabditis elegans] ref|NP_491746.1| C.Elegans Homeobox family member (ceh-2) [Caenorhabditis elegans] gb|AAO91836.1| homeodomain protein CEH-2 [Caenorhabditis elegans] gb|AAO91835.1| homeodomain protein CEH-2 [Caenorhabditis elegans] 2.0E-69
blast CAE72921 Hypothetical protein CBG20241 [Caenorhabditis briggsae] 3.0E-60
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 3.99999992980668E-13
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 3.99999987306209E-21
Pfam IPR001356 EMBL-EBI Homeobox 2.5E-30
Pfam IPR001356 EMBL-EBI Homeobox 5.1E-16
Sequence
Target Sequence 
>C. ELEGANS:191750_AT
aatgagccactttcacagacactctcatattttgacgttcttcttccgcatgttcaaatg
gcttgttcgaatccgtttatttcgggtattggagcatcaggcagtggagatcagaatttg
aatacaggtgctggcggatctgtatggcaacatccatggcttgaattgttgcaaagtacg
acagctgcacaattcggtgacgtcacagctgggctctttcttcaacctctccgaaaaaac
aaaagaattcgaacggcatttagtgcatctcaactgatacaactagaaaaggcattcgaa
ggaaatcattacgtcgtgggaaatgagcgaaaacagttggcagcaaagttgagcttaacg
gaaacacaggtaaaagtttggtttcaaaatcggcggaccaaacacaagagagtcagattg
gaaggatcagatccgaacgctccgatgtcaaacgacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGAGCCACTTTCACAGACACTCT 115 165 151 Antisense
GACACTCTCATATTTTGACGTTCTT 359 367 168 Antisense
TTCTTCTTCCGCATGTTCAAATGGC 450 689 188 Antisense
AATGGCTTGTTCGAATCCGTTTATT 467 161 207 Antisense
TTTCGGGTATTGGAGCATCAGGCAG 112 671 230 Antisense
GGTGCTGGCGGATCTGTATGGCAAC 601 501 277 Antisense
TGGCAACATCCATGGCTTGAATTGT 385 545 295 Antisense
GTACGACAGCTGCACAATTCGGTGA 120 459 326 Antisense
AATTCGGTGACGTCACAGCTGGGCT 619 177 341 Antisense
GAACGGCATTTAGTGCATCTCAACT 126 349 401 Antisense
CGAACGCTCCGATGTCAAACGACGA 201 253 584 Antisense
Sequence Source Affymetrix Proprietary Database