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[191748_at] ubiquitin-conjugating enzyme

Description C28G1.1 /REP_DB=WormBase Gene ID /WP=CE04118 /TR=Q18288 /GB=AAA82352.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 72      4grp_highest_8_8 > 04      4grp_high_8_8 > 38      6grp_highest_nonorm > 91      6grp_total > 72      4grp_total > 33      6grp_highest_sub > 04      6grp_cgc > 75      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 215.6
(-3)
P 		315.4
(3)
P 		266.1
(0)
P 		261.1
(-0.3)
P 		100
B 242.2
(-3)
P 		297.5
(3)
P 		268.6
(-0.1)
P 		292.6
(2.5)
P 		55
C 248.2
(-3)
P 		277.1
(0.4)
P 		298.9
(3)
P 		288.4
(1.8)
P 		51
avr 235.3
(-3) 		296.7
(3) 		277.9
(1.2) 		280.7
(1.4) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 181
1 2 3 4 5 6 range
A 148.5
(-3)
P 		230.4
(0.2)
P 		239.8
(0.5)
P 		286.2
(2.3)
P 		303
(3)
P 		180.9
(-1.7)
P 		155
B 176.2
(-3)
P 		182.6
(-2.8)
P 		277.7
(1)
P 		329.8
(3)
P 		236.7
(-0.6)
P 		186.3
(-2.6)
P 		154
C 182.6
(-3)
P 		216.1
(-0.7)
P 		270.4
(3)
P 		262.1
(2.4)
P 		261.3
(2.4)
P 		200.7
(-1.8)
P 		88
avr 169.1
(-3) 		209.7
(-1) 		262.6
(1.5) 		292.7
(3) 		267
(1.8) 		189.3
(-2) 		124
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 78.5
(3)
P 		68.5
(1.5)
P 		69.6
(1.7)
P 		38.2
(-3)
P 		40
B 77.2
(3)
P 		73.5
(1.5)
P 		62.2
(-3)
P 		62.3
(-3)
P 		15
C 82.1
(0.6)
P 		79.7
(0.4)
P 		104.2
(3)
P 		48.6
(-3)
P 		56
avr 79.3
(3) 		73.9
(1.9) 		78.7
(2.9) 		49.7
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 27.4
(-3)
P 		81.9
(2.1)
P 		52.2
(-0.7)
P 		72.6
(1.2)
P 		91.4
(3)
P 		44.1
(-1.4)
P 		64
B 61.3
(-2.9)
P 		60.8
(-3)
P 		67.4
(-1.9)
P 		87.4
(1.5)
P 		96.2
(3)
P 		69.5
(-1.5)
P 		35
C 67.7
(-0.2)
P 		65.9
(-0.5)
P 		68.8
(0)
P 		52.7
(-3)
P 		84.6
(3)
P 		67.2
(-0.3)
P 		32
avr 52.1
(-3) 		69.5
(-0.3) 		62.8
(-1.3) 		70.9
(-0.1) 		90.7
(3) 		60.3
(-1.7) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191748_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15971
Sequence Type Exemplar sequence
Representative Public ID C28G1.1 NCBI
Target Description C28G1.1 /REP_DB=WormBase Gene ID /WP=CE04118 /TR=Q18288 /GB=AAA82352.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077097(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077097 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-23) (ubc-23) mRNA, complete cds. 11/11 None
GENEFINDER00000026788 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:8844231:8848321:1 11/11 None
C28G1.1 ENSEMBL cdna:known chromosome:CEL140:X:8844381:8848321:1 gene:C28G1.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000026785 4/11 Cross Hyb Matching Probes None
SNAP00000026786 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:8844379-8847960(+) 93.83 100.0
Public Domain and Genome References
Gene Title ubiquitin-conjugating enzyme
Gene Symbol ubc-23
Ensembl C28G1.1
Entrez Gene 182989 Entrez gene
SwissProt Q18288 EMBL-EBI
WormBase CE37613 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA82352 Ubiquitin conjugating enzyme protein 23 [Caenorhabditis elegans] ref|NP_509498.2| UBiquitin Conjugating enzyme family member (ubc-23) [Caenorhabditis elegans] 0.0
blast CAE69517 Hypothetical protein CBG15726 [Caenorhabditis briggsae] 3.0E-32
blast AAB71287 Hypothetical protein F48A11.5a [Caenorhabditis elegans] ref|NP_001022180.1| F48A11.5a [Caenorhabditis elegans] 5.0E-32
blast AAB71287 Hypothetical protein F48A11.5a [Caenorhabditis elegans] ref|NP_001022180.1| F48A11.5a [Caenorhabditis elegans] 2.0E-31
blast AAT81192 Hypothetical protein F48A11.5b [Caenorhabditis elegans] ref|NP_001022181.1| F48A11.5b [Caenorhabditis elegans] 2.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 2.6E-43
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.8E-49
Sequence
Target Sequence 
>C. ELEGANS:191748_AT
gaaagtcataagcgttacacctcagaacctaccagaagaaccaaatgtttccgaaacaaa
tattgtcacagtgaagttccggttgccaaaagggatccagggaatcataaaaggagtgga
agaaacgccgtacgaaggtggtatttttgagcttgacatcaaaatcggagaatcttaccc
attcaaagctcccactgtaaaatttataaccaagatatggcatccatcagtgagcccatt
cgacgggaccatatgccttcatgatgtggacaatggagtttggccagtatctatgacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGTCATAAGCGTTACACCTCAG 149 361 1059 Antisense
TTACACCTCAGAACCTACCAGAAGA 534 683 1073 Antisense
GTCACAGTGAAGTTCCGGTTGCCAA 649 463 1123 Antisense
GTTCCGGTTGCCAAAAGGGATCCAG 644 303 1134 Antisense
AGGTGGTATTTTTGAGCTTGACATC 429 57 1194 Antisense
AGAATCTTACCCATTCAAAGCTCCC 190 73 1227 Antisense
CCATTCAAAGCTCCCACTGTAAAAT 33 271 1237 Antisense
TGGCATCCATCAGTGAGCCCATTCG 320 545 1276 Antisense
ATTCGACGGGACCATATGCCTTCAT 553 7 1296 Antisense
GACCATATGCCTTCATGATGTGGAC 179 383 1305 Antisense
GGAGTTTGGCCAGTATCTATGACAA 664 517 1333 Antisense
Sequence Source Affymetrix Proprietary Database