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[191737_at] Putative membrane protein

Description ZK849.4 /REP_DB=WormBase Gene ID /WP=CE25696 /TR=SW:O18303 /GB=CAB05027.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster 6grp_total > 29      4grp_total > 50      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 9.3
(-3)
A 		52.7
(3)
A 		43.6
(1.7)
A 		40.6
(1.3)
A 		43
B 32.3
(0.9)
A 		34.7
(1.2)
A 		48.8
(3)
A 		2.6
(-3)
A 		46
C 8
(-3)
A 		57
(3)
A 		28.8
(-0.5)
A 		10.4
(-2.7)
A 		49
avr 16.5
(-3) 		48.1
(3) 		40.4
(1.5) 		17.9
(-2.7) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 65
1 2 3 4 5 6 range
A 75.5
(3)
A 		23
(-2.9)
A 		28.7
(-2.2)
A 		33.3
(-1.7)
A 		21.8
(-3)
A 		38.6
(-1.1)
A 		54
B 40.5
(3)
A 		27.7
(0.5)
A 		10.2
(-3)
A 		32
(1.3)
A 		37.6
(2.4)
A 		12.8
(-2.5)
A 		30
C 36.3
(-0.7)
A 		28.3
(-1.6)
A 		21
(-2.5)
A 		16.3
(-3)
A 		68.7
(3)
A 		40.4
(-0.2)
A 		52
avr 50.8
(3) 		26.3
(-1.8) 		20
(-3) 		27.2
(-1.6) 		42.7
(1.4) 		30.6
(-0.9) 		31
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 3.4
(-3)
A 		11.4
(3)
A 		11.4
(3)
A 		5.9
(-1.1)
A 		8
B 10.3
(2.4)
A 		8.6
(1.5)
A 		11.3
(3)
A 		0.6
(-3)
A 		11
C 2.7
(-2.6)
A 		16.4
(3)
A 		10
(0.4)
A 		1.7
(-3)
A 		15
avr 5.5
(-1.2) 		12.1
(3) 		10.9
(2.2) 		2.7
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 13.9
(3)
A 		8.2
(-1.4)
A 		6.2
(-3)
A 		8.4
(-1.3)
A 		6.6
(-2.7)
A 		9.4
(-0.5)
A 		8
B 14.1
(2.4)
A 		9.2
(0.1)
A 		2.5
(-3)
A 		8.5
(-0.2)
A 		15.3
(3)
A 		4.8
(-1.9)
A 		13
C 13.5
(0.2)
A 		8.6
(-1.3)
A 		5.4
(-2.3)
A 		3.3
(-3)
A 		22.2
(3)
A 		13.5
(0.2)
A 		19
avr 13.8
(2.5) 		8.7
(-0.6) 		4.7
(-3) 		6.7
(-1.8) 		14.7
(3) 		9.2
(-0.3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191737_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1917
Sequence Type Exemplar sequence
Representative Public ID ZK849.4 NCBI
Target Description ZK849.4 /REP_DB=WormBase Gene ID /WP=CE25696 /TR=SW:O18303 /GB=CAB05027.2 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Putative membrane protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061078(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061078 NCBI Caenorhabditis elegans ZK849.4 (ZK849.4) mRNA, complete cds. 11/11 None
SNAP00000021693 ENSEMBL cdna:SNAP chromosome:CEL140:I:14213874:14216699:1 11/11 None
GENEFINDER00000021695 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14213874:14216699:1 11/11 None
ZK849.4 ENSEMBL cdna:known chromosome:CEL140:I:14213835:14216699:1 gene:ZK849.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14213765-14216630(+) 99.39 100.0
Public Domain and Genome References
Gene Title Putative membrane protein
Ensembl ZK849.4
Entrez Gene 191437 Entrez gene
SwissProt O18303 EMBL-EBI
WormBase CE25696 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB05027 Hypothetical protein ZK849.4 [Caenorhabditis elegans] ref|NP_493479.1| ZK849.4 [Caenorhabditis elegans] sp|O18303|YHS4_CAEEL Hypothetical protein ZK849.4 in chromosome I 0.0
blast T28037 hypothetical protein ZK849.4 - Caenorhabditis elegans 0.0
blast CAB05710 Hypothetical protein C49A1.2 [Caenorhabditis elegans] emb|CAB05029.1| Hypothetical protein C49A1.2 [Caenorhabditis elegans] ref|NP_493480.1| C49A1.2 [Caenorhabditis elegans] 6.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Pfam IPR000615 EMBL-EBI Bestrophin 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493479.1 3 51-73,318-337,350-369
ZK849.4 3 51-73,318-337,350-369
SNAP00000021693 3 38-60,305-324,337-356
GENEFINDER00000021695 3 38-60,305-324,337-356
Sequence
Target Sequence 
>C. ELEGANS:191737_AT
aagaatctctacattggactggcgattgtggatacagagtgtggaaaacatccggaaatt
gtgaaggatacaattggcaaagactgccttccattctatccacaaaacgatgatgataat
gagaggaatcgcgcacttgtcggctcgacgaagaatatcaatttgtctggacgatctgac
accccagccgcttcaccttcggtgttctatgtcaatatcaatgtcaaggtcgagaaccct
accccagtcattgtcgatggcgcccgacctcctccatctccaccagctcttccgccagcg
cttccaaatccaaatgaaccacctccacctccacctccggctgaagctctccgtgctgac
caacctcgtcattctctgattcatgaagtgggcgcccaagctgatccggccgaggctaat
ggtgcccaagctctctcacccgtgcttccaaatccaaatgaacgtgctgacgaacatggt
cattctccggtcattgacaatgaggcccaacctagtccacctccggccgaggccaatcgt
gctccgattccaaatcaacaaccccaggtcaatggtaacgccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGAATCTCTACATTGGACTGGCGA 503 151 1192 Antisense
ATGAGAGGAATCGCGCACTTGTCGG 58 45 1310 Antisense
ATTTGTCTGGACGATCTGACACCCC 79 19 1352 Antisense
TCACCTTCGGTGTTCTATGTCAATA 396 625 1384 Antisense
AACCTCGTCATTCTCTGATTCATGA 309 147 1553 Antisense
ATGAAGTGGGCGCCCAAGCTGATCC 164 43 1574 Antisense
TGATCCGGCCGAGGCTAATGGTGCC 361 563 1593 Antisense
GACGAACATGGTCATTCTCCGGTCA 57 379 1660 Antisense
TGACAATGAGGCCCAACCTAGTCCA 47 573 1686 Antisense
ATCGTGCTCCGATTCCAAATCAACA 328 35 1727 Antisense
CAACCCCAGGTCAATGGTAACGCCG 395 185 1750 Antisense
Sequence Source Affymetrix Proprietary Database