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[191731_s_at]

Description C14B9.4 /REP_DB=WormBase Gene ID /WP=CE26649 /GEN=plk-1 /TR=SW:P34331 /GB=AAA27947.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster 6grp_highest_10_10 > 87      6grp_high1_10_10 > 78      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 98      6grp_total > 78      4grp_total > 59      6grp_highest_sub > Da      6grp_cgc > 49      6grp_high1_sub > Cu      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 975
1 2 3 4 range
A 988.6
(2.5)
P 		1049.2
(3)
P 		992.1
(2.5)
P 		310.2
(-3)
P 		739
B 699.3
(-1.3)
P 		1171.8
(2.9)
P 		1183.6
(3)
P 		509.4
(-3)
P 		674
C 792.6
(-0)
P 		1138.5
(2.1)
P 		1280.9
(3)
P 		306
(-3)
P 		975
avr 826.8
(0.5) 		1119.8
(2.7) 		1152.2
(3) 		375.2
(-3) 		777

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1571
1 2 3 4 5 6 range
A 659
(-2.1)
P 		780.2
(-1.5)
P 		459.3
(-3)
P 		560.1
(-2.5)
P 		1135.1
(0.2)
P 		1731.9
(3)
P 		1273
B 639
(-2.5)
P 		600.3
(-2.7)
P 		517.6
(-3)
P 		713.4
(-2.2)
P 		1136.1
(-0.5)
P 		2030.4
(3)
P 		1513
C 983.3
(-0.9)
P 		515
(-3)
P 		528
(-2.9)
P 		547.5
(-2.9)
P 		1101.8
(-0.4)
P 		1869.8
(3)
P 		1355
avr 760.4
(-1.9) 		631.8
(-2.4) 		501.6
(-3) 		607
(-2.5) 		1124.3
(-0.3) 		1877.4
(3) 		1376
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 401
1 2 3 4 range
A 359.9
(3)
P 		227.8
(0.5)
P 		259.4
(1.1)
P 		45.4
(-3)
P 		315
B 223
(0.8)
P 		289.4
(3)
P 		274.3
(2.5)
P 		108.6
(-3)
P 		181
C 262.4
(0.2)
P 		327.3
(1.2)
P 		446.4
(3)
P 		51.6
(-3)
P 		395
avr 281.8
(2) 		281.5
(1.9) 		326.7
(3) 		68.5
(-3) 		258

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 657
1 2 3 4 5 6 range
A 121.5
(-2.6)
P 		277.5
(0.3)
P 		100
(-3)
P 		142
(-2.2)
P 		342.5
(1.5)
P 		421.7
(3)
P 		322
B 222.3
(-2.1)
P 		199.8
(-2.3)
P 		125.7
(-3)
P 		189
(-2.4)
P 		461.6
(0.2)
P 		757.1
(3)
P 		631
C 364.5
(-0)
P 		157.1
(-2.5)
P 		134.4
(-2.7)
P 		110.1
(-3)
P 		356.5
(-0.1)
P 		625.9
(3)
P 		516
avr 236.1
(-1.6) 		211.5
(-1.9) 		120
(-3) 		147
(-2.7) 		386.9
(0.3) 		601.6
(3) 		482
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191731_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7456
Sequence Type Exemplar sequence
Representative Public ID C14B9.4 NCBI
Target Description C14B9.4 /REP_DB=WormBase Gene ID /WP=CE26649 /GEN=plk-1 /TR=SW:P34331 /GB=AAA27947.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026002(11), NM_001026003(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026002 NCBI Caenorhabditis elegans POLO Kinase family member (plk-1) (plk-1) mRNA, complete cds. 11/11 None
NM_001026003 NCBI Caenorhabditis elegans POLO Kinase family member (plk-1) (plk-1) mRNA, complete cds. 11/11 None
SNAP00000018766 ENSEMBL cdna:SNAP chromosome:CEL140:III:8101269:8103648:1 11/11 None
GENEFINDER00000018778 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8101269:8103648:1 11/11 None
C14B9.4a.2 ENSEMBL cdna:known chromosome:CEL140:III:8101268:8103992:1 gene:C14B9.4 11/11 None
C14B9.4b ENSEMBL cdna:known chromosome:CEL140:III:8101269:8103997:1 gene:C14B9.4 11/11 A 
C14B9.4a.1 ENSEMBL cdna:known chromosome:CEL140:III:8101269:8103992:1 gene:C14B9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8102387-8103646(+) 100.0 100.0
Public Domain and Genome References
Ensembl C14B9.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA27947 Polo kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001021173.1| POLO Kinase family member (plk-1) [Caenorhabditis elegans] gb|AAC34661.1| polo-like kinase-1; PLK-1 [Caenorhabditis elegans] 0.0
blast AAM22025 Polo kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001021174.1| POLO Kinase family member (plk-1) [Caenorhabditis elegans] gb|AAC14129.1| putative serine/threonine protein kinase [Caenorhabditis elegans] sp|P34331|PLK1_CAEEL Serine/threonine-protein kinase plk-1 (Polo-like kinase-1) 0.0
blast CAE64506 Hypothetical protein CBG09233 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-59
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 5.0E-71
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 6.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR000959 EMBL-EBI POLO box duplicated region 2.3E-33
Pfam IPR000959 EMBL-EBI POLO box duplicated region 3.0E-24
Pfam IPR000719 EMBL-EBI Protein kinase 3.0E-82
Pfam IPR000959 EMBL-EBI POLO box duplicated region 2.3E-33
Pfam IPR000959 EMBL-EBI POLO box duplicated region 3.0E-24
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-78
Sequence
Target Sequence 
>C. ELEGANS:191731_S_AT
gacaattctcgtatcatgcttgatcaagctggaaacgagttgacctatattgagaagagc
aacaaagagcactacttctcgatgcactcaggtgaaatgcctggattgctcaacaagaaa
gtcacattgcttaagtattttcgatcctacatgaatgaccatttggtgaaagctggagaa
ggatctgaacaaagagctggtgatgacttggctcgtttaccaacgcttcgagtttggttc
agaaccaaatcagcaattgttttgcacctttccaatggaactgttcagataaatttcttc
aatgatcatgtcaagatgatgatgtgcccattaatgcaagcagttacattcatcgatcaa
aacaagcgtatgctcacctacaagctgaacaatcttcagcgcaatggatgtccagagaaa
ttccttcaccgactgaagtatgcgaagacaatgatcgaaaggttgatgagcgatgctaac
gttgtatcccagaatccagctcgtcaaccggatatgccacggtcaatggcagctgctcgt
tcagcttcggcaggatcacgaggaccaaatcaagctgcatcgcacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAATTCTCGTATCATGCTTGATC 668 363 499 Antisense
CACTACTTCTCGATGCACTCAGGTG 659 195 568 Antisense
GGTGAAATGCCTGGATTGCTCAACA 450 501 589 Antisense
ATGACTTGGCTCGTTTACCAACGCT 98 45 701 Antisense
GCAATTGTTTTGCACCTTTCCAATG 471 323 751 Antisense
AAGCGTATGCTCACCTACAAGCTGA 39 151 862 Antisense
GTCCAGAGAAATTCCTTCACCGACT 126 477 908 Antisense
GCTAACGTTGTATCCCAGAATCCAG 497 299 973 Antisense
AGCTCGTCAACCGGATATGCCACGG 578 79 996 Antisense
CGTTCAGCTTCGGCAGGATCACGAG 646 247 1036 Antisense
GGACCAAATCAAGCTGCATCGCACC 362 523 1060 Antisense
Sequence Source Affymetrix Proprietary Database