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[191728_at] protein kinase

Description Y54E10BL.6 /REP_DB=WormBase Gene ID /WP=CE25437 /GEN=mek-2 /TR=Q10664 /GB=AAF60779.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_total > 24      4grp_total > 39      6grp_cgc > 30      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 262.6
(3)
M 		123.4
(-3)
A 		137
(-2.4)
A 		189.1
(-0.2)
A 		139
B 268.9
(3)
M 		138.9
(-1.6)
A 		99.5
(-3)
A 		192.7
(0.3)
A 		169
C 246.7
(3)
A 		138.9
(-3)
A 		147.3
(-2.5)
A 		159
(-1.9)
A 		108
avr 259.4
(3) 		133.7
(-2.7) 		127.9
(-3) 		180.3
(-0.6) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 100
1 2 3 4 5 6 range
A 134.8
(1.2)
A 		132.2
(1.1)
A 		134.1
(1.2)
A 		161.5
(3)
A 		95.4
(-1.4)
A 		70.8
(-3)
A 		91
B 158.8
(3)
A 		132
(1.4)
A 		104.8
(-0.3)
A 		116.8
(0.4)
A 		61.2
(-3)
A 		115.4
(0.3)
A 		98
C 144.8
(2)
A 		160.3
(3)
A 		136.4
(1.5)
A 		124
(0.7)
A 		65.6
(-3)
A 		112.8
(-0)
A 		95
avr 146.1
(3) 		141.5
(2.6) 		125.1
(1.3) 		134.1
(2) 		74.1
(-3) 		99.7
(-0.9) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 95.6
(3)
M 		26.8
(-3)
A 		35.8
(-2.2)
A 		27.7
(-2.9)
A 		69
B 85.7
(3)
M 		34.3
(-1.9)
A 		23.1
(-3)
A 		41.1
(-1.3)
A 		63
C 81.7
(3)
A 		39.9
(-1.6)
A 		51.4
(-0.3)
A 		26.8
(-3)
A 		55
avr 87.7
(3) 		33.7
(-2.8) 		36.8
(-2.5) 		31.9
(-3) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 24.8
(-1.5)
A 		47
(3)
A 		29.2
(-0.6)
A 		41
(1.8)
A 		28.8
(-0.7)
A 		17.2
(-3)
A 		30
B 55.3
(3)
A 		43.9
(0.8)
A 		25.4
(-2.9)
A 		30.9
(-1.8)
A 		24.9
(-3)
A 		43
(0.6)
A 		30
C 53.7
(3)
A 		48.9
(2.1)
A 		34.7
(-0.5)
A 		24.9
(-2.3)
A 		21.2
(-3)
A 		37.8
(0.1)
A 		33
avr 44.6
(2.4) 		46.6
(3) 		29.8
(-1.7) 		32.3
(-1) 		25
(-3) 		32.7
(-0.9) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191728_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.223
Sequence Type Exemplar sequence
Representative Public ID Y54E10BL.6 NCBI
Target Description Y54E10BL.6 /REP_DB=WormBase Gene ID /WP=CE25437 /GEN=mek-2 /TR=Q10664 /GB=AAF60779.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058686(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058686 NCBI Caenorhabditis elegans MAP kinase kinase or Erk Kinase family member (mek-2) (mek-2) mRNA, complete cds. 11/11 A 
SNAP00000040893 ENSEMBL cdna:SNAP chromosome:CEL140:I:3001465:3013446:-1 11/11 None
GENEFINDER00000040902 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3006030:3009323:-1 11/11 None
Y54E10BL.6 ENSEMBL cdna:known chromosome:CEL140:I:3005428:3013449:-1 gene:Y54E10BL.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3006029-3013446(-) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Gene Symbol mek-2
Ensembl Y54E10BL.6
Entrez Gene 171872 Entrez gene
SwissProt Q10664 EMBL-EBI
WormBase CE25437 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16061_S_AT mitogen-activated protein kinase kinase (MAPKK) (MKK1) (MEK1) at
ATH1-121501:253993_AT mitogen-activated protein kinase kinase (MAPKK) (MKK1) (MEK1) at
CANINE:1591778_S_AT similar to Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) cfa
CANINE:1589151_S_AT similar to Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) cfa
CANINE:1587276_AT similar to Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) cfa
CANINE_2:CFA.1354.1.A1_AT similar to Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) cfa
CANINE_2:CFA.13955.1.A1_AT similar to Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) cfa
CANINE_2:CFAAFFX.26470.1.S1_S_AT similar to Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK1) cfa
DROSOPHILA_2:1626006_AT Downstream of raf1 dm
DROSGENOME1:154939_AT Downstream of raf1 dm
CHICKEN:GGA.1443.1.S1_S_AT mitogen-activated protein kinase kinase 1 gga
CHICKEN:GGA.1443.2.S1_S_AT mitogen-activated protein kinase kinase 1 gga
HG-U95AV2:1130_AT mitogen-activated protein kinase kinase 1 hs
HC-G110:1130_AT mitogen-activated protein kinase kinase 1 hs
HU35KSUBB:RC_AA169475_AT mitogen-activated protein kinase kinase 1 hs
HG-U95AV2:1844_S_AT mitogen-activated protein kinase kinase 1 hs
HC-G110:1844_S_AT mitogen-activated protein kinase kinase 1 hs
HUGENEFL:L11284_AT mitogen-activated protein kinase kinase 1 hs
HUGENEFL:L05624_S_AT mitogen-activated protein kinase kinase 1 hs
U133_X3P:HS.3446.0.A2_3P_AT mitogen-activated protein kinase kinase 1 hs
HG-U133A_2:202670_AT mitogen-activated protein kinase kinase 1 hs
HG-FOCUS:202670_AT mitogen-activated protein kinase kinase 1 hs
HG-U133_PLUS_2:202670_AT mitogen-activated protein kinase kinase 1 hs
HG-U133A:202670_AT mitogen-activated protein kinase kinase 1 hs
HG-U95C:60945_AT mitogen-activated protein kinase kinase 1 hs
U133_X3P:HS.30174.1.S2_3P_AT Mitogen-activated protein kinase kinase 1 hs
HG-U133A_2:214592_S_AT Mitogen-activated protein kinase kinase 1 hs
HG-U133_PLUS_2:214592_S_AT Mitogen-activated protein kinase kinase 1 hs
HG-U133A:214592_S_AT Mitogen-activated protein kinase kinase 1 hs
MU11KSUBA:L02526_S_AT mitogen activated protein kinase kinase 1 mm
MG-U74AV2:92585_AT mitogen activated protein kinase kinase 1 mm
MOE430A:1416351_AT mitogen activated protein kinase kinase 1 mm
MOUSE430A_2:1416351_AT mitogen activated protein kinase kinase 1 mm
MOUSE430_2:1416351_AT mitogen activated protein kinase kinase 1 mm
MU19KSUBA:TC19248_AT Mitogen activated protein kinase kinase 1 (Map2k1), mRNA mm
MU19KSUBA:TC23099_AT Mitogen activated protein kinase kinase 1 (Map2k1), mRNA mm
MU19KSUBC:TC35982_AT Mitogen activated protein kinase kinase 1 (Map2k1), mRNA mm
RT-U34:L04485MRNA_S_AT mitogen activated protein kinase kinase 1 rn
RN-U34:L04485MRNA_S_AT mitogen activated protein kinase kinase 1 rn
RG-U34A:L04485MRNA_S_AT mitogen activated protein kinase kinase 1 rn
RN-U34:D14591_S_AT mitogen activated protein kinase kinase 1 rn
RT-U34:D14591_S_AT mitogen activated protein kinase kinase 1 rn
RG-U34A:D14591_S_AT mitogen activated protein kinase kinase 1 rn
RAE230A:1367760_AT mitogen activated protein kinase kinase 1 rn
RAT230_2:1367760_AT mitogen activated protein kinase kinase 1 rn
RN-U34:RC_AI178835_AT mitogen activated protein kinase kinase 1 rn
RT-U34:RC_AI178835_AT mitogen activated protein kinase kinase 1 rn
RG-U34A:RC_AI178835_AT mitogen activated protein kinase kinase 1 rn
RAE230B:1380729_AT Mitogen activated protein kinase kinase 1 rn
RAT230_2:1380729_AT Mitogen activated protein kinase kinase 1 rn
YEAST_2:1777178_AT MAP kinase kinase that plays a pivotal role in the osmosensing signal-transduction pathway, activated under severe osmotic stress Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60779 Map kinase kinase or erk kinase protein 2 [Caenorhabditis elegans] ref|NP_491087.1| MAP kinase kinase or Erk Kinase family member (mek-2) [Caenorhabditis elegans] sp|Q10664|MEK2_CAEEL Dual specificity mitogen-activated protein kinase kinase mek-2 (MAP kinase kinase mek-2) gb|AAA85118.1| MAP kinase kinase 0.0
blast CAE60561 Hypothetical protein CBG04190 [Caenorhabditis briggsae] 0.0
blast AAF60779 Map kinase kinase or erk kinase protein 2 [Caenorhabditis elegans] ref|NP_491087.1| MAP kinase kinase or Erk Kinase family member (mek-2) [Caenorhabditis elegans] sp|Q10664|MEK2_CAEEL Dual specificity mitogen-activated protein kinase kinase mek-2 (MAP kinase kinase mek-2) gb|AAA85118.1| MAP kinase kinase 1.0E-124
blast CAD56893 MAP kinase kinase 2 [Meloidogyne artiellia] 1.0E-121
blast CAE60561 Hypothetical protein CBG04190 [Caenorhabditis briggsae] 1.0E-118
blast AAF60779 Map kinase kinase or erk kinase protein 2 [Caenorhabditis elegans] ref|NP_491087.1| MAP kinase kinase or Erk Kinase family member (mek-2) [Caenorhabditis elegans] sp|Q10664|MEK2_CAEEL Dual specificity mitogen-activated protein kinase kinase mek-2 (MAP kinase kinase mek-2) gb|AAA85118.1| MAP kinase kinase 6.0E-92
blast CAE60561 Hypothetical protein CBG04190 [Caenorhabditis briggsae] 5.0E-89
Protein Families
Method ID Description E-Value
hanks 1.1.12 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); TPK1 3.0E-21
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.6E-67
Pfam IPR000719 EMBL-EBI Protein kinase 3.6E-20
Pfam IPR000719 EMBL-EBI Protein kinase 7.2E-41
Pfam IPR000719 EMBL-EBI Protein kinase 2.0E-38
Sequence
Target Sequence 
>C. ELEGANS:191728_AT
gattgattcgatggccaactcatttgtcggaacgaggagttatatggcgcccgaacgact
cacaggatcccactatacaatttcatcggatatctggtcatttggattatcacttgtcga
acttcttattggacgatatccagtgcctgcaccgtctcaagcagaatatgctacaatgtt
taatgtggctgaaaatgagattgaactggctgatagtcttgaagagcccaactatcatcc
gccgagcaatccggcgtcaatggctatttttgaaatgcttgattatattgttaatggacc
tccgccaacacttccgaagagattctttactgacgaagtgattggatttgtgtcgaaatg
ccttcgaaaacttccaagtgaacgagcaaccctaaaatctctcaccgcagacgtcttctt
cacacaatatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTGATTCGATGGCCAACTCATTT 225 435 672 Antisense
GAGTTATATGGCGCCCGAACGACTC 589 397 708 Antisense
GACTCACAGGATCCCACTATACAAT 708 371 728 Antisense
GGATTATCACTTGTCGAACTTCTTA 579 507 775 Antisense
ATTGGACGATATCCAGTGCCTGCAC 295 3 799 Antisense
CTTGAAGAGCCCAACTATCATCCGC 255 217 889 Antisense
ATCATCCGCCGAGCAATCCGGCGTC 4 29 905 Antisense
CAATCCGGCGTCAATGGCTATTTTT 596 181 918 Antisense
ATATTGTTAATGGACCTCCGCCAAC 129 17 956 Antisense
CGAGCAACCCTAAAATCTCTCACCG 458 249 1054 Antisense
CAGACGTCTTCTTCACACAATATGC 624 205 1079 Antisense
Sequence Source Affymetrix Proprietary Database