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[191727_at] Ubiquitin-conjugating enzyme

Description D1022.1 /REP_DB=WormBase Gene ID /WP=CE02575 /TR=Q18931 /GB=AAB93334.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Ubiquitin-conjugating enzyme
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 49      4grp_highest_8_8 > 54      4grp_high_8_8 > 10      6grp_highest_nonorm > 86      6grp_total > 59      4grp_total > 22      6grp_highest_sub > Cu      6grp_cgc > 09      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 450
1 2 3 4 range
A 1322.3
(3)
P 		926
(-3)
P 		1016.6
(-1.6)
P 		1298
(2.6)
P 		396
B 1197.1
(3)
P 		1097.1
(0.1)
P 		988.3
(-3)
P 		1135.6
(1.2)
P 		209
C 1171.8
(1.3)
P 		1051.4
(-0.5)
P 		872.1
(-3)
P 		1294.8
(3)
P 		423
avr 1230.4
(2.7) 		1024.8
(-1.6) 		959
(-3) 		1242.8
(3) 		284

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 543
1 2 3 4 5 6 range
A 1374.7
(3)
P 		831.5
(-3)
P 		936.3
(-1.8)
P 		908.2
(-2.2)
P 		1096.8
(-0.1)
P 		1084.2
(-0.2)
P 		543
B 903
(-1.8)
P 		972.1
(-0.4)
P 		845.8
(-3)
P 		1140.2
(3)
P 		1057.8
(1.3)
P 		1014.8
(0.4)
P 		294
C 870.4
(-2.8)
P 		1076.3
(1.7)
P 		881.2
(-2.6)
P 		1134
(3)
P 		861
(-3)
P 		1089.5
(2)
P 		273
avr 1049.4
(2.5) 		960
(-0.5) 		887.8
(-3) 		1060.8
(2.9) 		1005.2
(1) 		1062.8
(3) 		175
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 292
1 2 3 4 range
A 481.5
(3)
P 		201
(-2.8)
P 		265.8
(-1.4)
P 		190
(-3)
P 		292
B 381.7
(3)
P 		271
(-1.3)
P 		229
(-3)
P 		242
(-2.5)
P 		153
C 387.9
(3)
P 		302.3
(-0)
P 		304
(0)
P 		218.3
(-3)
P 		170
avr 417
(3) 		258.1
(-1.8) 		266.3
(-1.5) 		216.8
(-3) 		200

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 226
1 2 3 4 5 6 range
A 253.4
(-0.7)
P 		295.7
(1.3)
P 		203.9
(-3)
P 		230.3
(-1.8)
P 		331
(3)
P 		264
(-0.2)
P 		127
B 314.2
(-0.1)
P 		323.6
(0.2)
P 		205.4
(-3)
P 		302
(-0.4)
P 		429.8
(3)
P 		378.4
(1.6)
P 		224
C 322.6
(1.2)
P 		328.4
(1.4)
P 		224.3
(-3)
P 		228
(-2.8)
P 		278.6
(-0.7)
P 		364.7
(3)
P 		140
avr 296.7
(0.8) 		315.9
(1.6) 		211.2
(-3) 		253.4
(-1.1) 		346.5
(3) 		335.7
(2.5) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191727_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5985
Sequence Type Exemplar sequence
Representative Public ID D1022.1 NCBI
Target Description D1022.1 /REP_DB=WormBase Gene ID /WP=CE02575 /TR=Q18931 /GB=AAB93334.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Ubiquitin-conjugating enzyme
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063165(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063165 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-6) (ubc-6) mRNA, complete cds. 11/11 None
SNAP00000004649 ENSEMBL cdna:SNAP chromosome:CEL140:II:7479201:7480434:1 11/11 None
GENEFINDER00000004659 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7479371:7480434:1 11/11 None
D1022.1 ENSEMBL cdna:known chromosome:CEL140:II:7479117:7480643:1 gene:D1022.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7479301-7480431(+) 100.0 100.0
Public Domain and Genome References
Gene Title Ubiquitin-conjugating enzyme
Gene Symbol ubc-6
Ensembl D1022.1
Entrez Gene 174217 Entrez gene
SwissProt Q18931 EMBL-EBI
WormBase CE29241 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257893_AT ubiquitin-conjugating enzyme, putative at
CANINE:1586094_S_AT similar to ubiquitin-conjugating enzyme E2, J1 cfa
CANINE:1586093_AT similar to ubiquitin-conjugating enzyme E2, J1 cfa
CANINE:1586092_AT similar to ubiquitin-conjugating enzyme E2, J1 cfa
CANINE_2:CFA.11661.1.A1_S_AT similar to ubiquitin-conjugating enzyme E2, J1 cfa
CANINE_2:CFAAFFX.5594.1.S1_AT similar to ubiquitin-conjugating enzyme E2, J1 cfa
CANINE_2:CFAAFFX.5595.1.S1_S_AT similar to ubiquitin-conjugating enzyme E2, J1 cfa
CHICKEN:GGA.4478.1.S1_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) gga
CHICKEN:GGA.4478.1.S2_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) gga
HG-U95AV2:39039_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133B:222435_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133_PLUS_2:222435_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133_PLUS_2:217826_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A:217826_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A_2:217826_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133_PLUS_2:217825_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A:217825_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A_2:217825_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A_2:217824_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133_PLUS_2:217824_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A:217824_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U95AV2:39040_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133_PLUS_2:217823_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A_2:217823_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133A:217823_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBA:W28360_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBA:D82419_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBB:RC_N20047_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBC:RC_N74950_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBC:RC_AA256528_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBC:RC_AA150043_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
U133_X3P:G7706311_3P_A_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
U133_X3P:G7106799_3P_A_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
U133_X3P:G6841527_3P_S_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
U133_X3P:HS.184325.0.A5_3P_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
U133_X3P:HS.184325.0.A3_3P_A_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U95B:57081_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U95C:57780_AT ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133_PLUS_2:228924_S_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133B:228924_S_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133_PLUS_2:236528_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U133B:236528_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U95C:65749_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HG-U95D:84553_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBA:H43996_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
HU35KSUBD:RC_AA258818_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
U133_X3P:HS.184325.1.A1_3P_A_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
U133_X3P:HS.313332.0.A1_3P_AT Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) hs
MG-U74BV2:111887_AT ubiquitin-conjugating enzyme E2, J1 mm
MG-U74CV2:165449_F_AT ubiquitin-conjugating enzyme E2, J1 mm
MOE430A:1448824_AT ubiquitin-conjugating enzyme E2, J1 mm
MOUSE430A_2:1448824_AT ubiquitin-conjugating enzyme E2, J1 mm
MOUSE430_2:1448824_AT ubiquitin-conjugating enzyme E2, J1 mm
MOE430A:1417723_AT ubiquitin-conjugating enzyme E2, J1 mm
MOUSE430A_2:1417723_AT ubiquitin-conjugating enzyme E2, J1 mm
MOUSE430_2:1417723_AT ubiquitin-conjugating enzyme E2, J1 mm
MU19KSUBB:TC34326_AT Ubiquitin-conjugating enzyme E2, J1, mRNA (cDNA clone MGC:25635 IMAGE:4217620) mm
MU19KSUBC:TC37547_AT Ubiquitin-conjugating enzyme E2, J1, mRNA (cDNA clone MGC:25635 IMAGE:4217620) mm
RG-U34B:RC_AI012638_AT ubiquitin-conjugating enzyme E2, J1 (predicted) rn
RAE230A:1373495_AT ubiquitin-conjugating enzyme E2, J1 (predicted) rn
RAT230_2:1373495_AT ubiquitin-conjugating enzyme E2, J1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495566 UBiquitin Conjugating enzyme family member (ubc-6) [Caenorhabditis elegans] gb|AAB93334.2| Ubiquitin conjugating enzyme protein 6 [Caenorhabditis elegans] 1.0E-162
blast NP_495566 UBiquitin Conjugating enzyme family member (ubc-6) [Caenorhabditis elegans] gb|AAB93334.2| Ubiquitin conjugating enzyme protein 6 [Caenorhabditis elegans] 1.0E-146
blast CAE67530 Hypothetical protein CBG13052 [Caenorhabditis briggsae] 1.0E-113
blast CAE67530 Hypothetical protein CBG13052 [Caenorhabditis briggsae] 1.0E-100
blast CAB83196 Non-Canonical UBiquitin Conjugating Enzyme 1 (NCUBE1) [Gallus gallus] ref|NP_990094.1| ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) [Gallus gallus] 4.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 1.4E-27
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 5.6E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495566.1 1 278-300
D1022.1 1 278-300
SNAP00000004649 1 278-300
GENEFINDER00000004659 1 251-273
Sequence
Target Sequence 
>C. ELEGANS:191727_AT
ttctcgcgttgattggattcctgccaagtaccccaggaggcgcgcttggtagtttggatt
atcctcccaaagagcgccagcgtttagccaaattgagctgtgagtggaaatgtaaagaat
gcggatgtgtcatgaaaactgcgcttcttccgataactgaagatggacaattaaaacaaa
ccgaagaagcgaaaactcttgctgcccaactcaagtttcaagatgaatctgttgttaaaa
aagaagttgaagcagccaacaatcaaaagaacccgacagaaacagagccaagtgaagaaa
caagctcggtcccaacagaaaatgtcgaagaatcagaagaagatgcagatgagagagaag
aaggtactactgttaatgtcaattcatcagaagtaccagatgtcgcacaacctgctgtcc
aacccattcgagatccacaacctctggtattccaacaccacgctccccgcttggcatcaa
caaactttgactatacccttctatacaagattccagttatcgctttgtgtttcgctattt
tcttcacactattggctcgtcgcttccttgtcgataatcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTCGCGTTGATTGGATTCCTGCC 563 693 332 Antisense
GGCGCGCTTGGTAGTTTGGATTATC 168 543 370 Antisense
TGGATTATCCTCCCAAAGAGCGCCA 618 549 386 Antisense
GCGCCAGCGTTTAGCCAAATTGAGC 449 287 405 Antisense
TCTTGCTGCCCAACTCAAGTTTCAA 223 619 528 Antisense
AAGTACCAGATGTCGCACAACCTGC 334 155 722 Antisense
GATCCACAACCTCTGGTATTCCAAC 246 415 763 Antisense
ACAAACTTTGACTATACCCTTCTAT 24 113 811 Antisense
ATCGCTTTGTGTTTCGCTATTTTCT 350 37 850 Antisense
GCTATTTTCTTCACACTATTGGCTC 504 303 865 Antisense
TCGTCGCTTCCTTGTCGATAATCTA 344 605 888 Antisense
Sequence Source Affymetrix Proprietary Database