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[191726_at]

Description Y95B8A.12 /REP_DB=WormBase Gene ID /WP=CE23161 /TR=Q9N2V4 /GB=AAF60897.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=nucleotide exchange factor
Cluster 6grp_total > 06      4grp_total > 30      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 501
1 2 3 4 range
A 633.6
(3)
P 		260.2
(-1.7)
M 		155.4
(-3)
A 		364.2
(-0.4)
P 		478
B 597.5
(3)
P 		331.2
(-0.4)
A 		132.9
(-3)
A 		479.8
(1.5)
P 		465
C 524.8
(3)
P 		197.1
(-2.3)
A 		151.9
(-3)
A 		442.6
(1.7)
P 		373
avr 585.3
(3) 		262.8
(-1.4) 		146.7
(-3) 		428.9
(0.9) 		439

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 474
1 2 3 4 5 6 range
A 518.1
(3)
P 		377.9
(0.8)
P 		164.1
(-2.5)
P 		196.2
(-2)
P 		172.1
(-2.3)
A 		129.5
(-3)
A 		389
B 472.6
(3)
P 		282.6
(-0.1)
P 		160.2
(-2.1)
P 		185.1
(-1.7)
M 		163.2
(-2.1)
M 		108.6
(-3)
A 		364
C 550
(3)
P 		287.9
(-0.3)
P 		288.5
(-0.3)
P 		132.6
(-2.3)
A 		213.5
(-1.3)
P 		75.6
(-3)
A 		474
avr 513.6
(3) 		316.1
(0.1) 		204.3
(-1.5) 		171.3
(-2) 		182.9
(-1.9) 		104.6
(-3) 		409
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 200
1 2 3 4 range
A 230.7
(3)
P 		56.5
(-2.5)
M 		40.6
(-3)
A 		53.3
(-2.6)
P 		190
B 190.5
(3)
P 		81.8
(-1.1)
A 		30.8
(-3)
A 		102.2
(-0.3)
P 		160
C 173.7
(3)
P 		56.7
(-2.8)
A 		52.9
(-3)
A 		74.6
(-1.9)
P 		121
avr 198.3
(3) 		65
(-2.1) 		41.4
(-3) 		76.7
(-1.7) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 179
1 2 3 4 5 6 range
A 95.5
(0.7)
P 		134.4
(3)
P 		35.7
(-2.8)
P 		49.8
(-1.9)
P 		51.9
(-1.8)
A 		31.5
(-3)
A 		103
B 164.4
(3)
P 		94.1
(-0.4)
P 		38.9
(-3)
P 		49
(-2.5)
M 		66.3
(-1.7)
M 		40.5
(-2.9)
A 		126
C 203.8
(3)
P 		87.8
(-0.9)
P 		73.4
(-1.4)
P 		26.7
(-3)
A 		69.1
(-1.5)
P 		25.3
(-3)
A 		179
avr 154.6
(3) 		105.4
(0.6) 		49.3
(-2.2) 		41.8
(-2.5) 		62.4
(-1.5) 		32.4
(-3) 		122
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191726_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19
Sequence Type Exemplar sequence
Representative Public ID Y95B8A.12 NCBI
Target Description Y95B8A.12 /REP_DB=WormBase Gene ID /WP=CE23161 /TR=Q9N2V4 /GB=AAF60897.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=nucleotide exchange factor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058391(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058391 NCBI Caenorhabditis elegans Y95B8A.12 (Y95B8A.12) mRNA, complete cds. 11/11 None
SNAP00000034258 ENSEMBL cdna:SNAP chromosome:CEL140:I:912443:919139:-1 11/11 None
GENEFINDER00000034286 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:912443:920139:-1 11/11 None
Y95B8A.12 ENSEMBL cdna:known chromosome:CEL140:I:912417:919149:-1 gene:Y95B8A.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:912442-920139(-) 99.45 100.0
Public Domain and Genome References
Ensembl Y95B8A.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60897 Hypothetical protein Y95B8A.12a [Caenorhabditis elegans] ref|NP_490792.2| Y95B8A.12 [Caenorhabditis elegans] 0.0
blast ABA00174 Hypothetical protein Y95B8A.12b [Caenorhabditis elegans] 0.0
blast CAE61298 Hypothetical protein CBG05122 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.9E-13
Pfam IPR000219 EMBL-EBI DH domain 4.9E-16
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.9E-13
Pfam IPR000219 EMBL-EBI DH domain 4.9E-16
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.9E-13
Pfam IPR000219 EMBL-EBI DH domain 4.9E-16
Sequence
Target Sequence 
>C. ELEGANS:191726_AT
ccttatcccgtcaattctgcgagaaccttctggctcatccgcgtcgaaagcttatccacg
aaggccccctcgagcttgtggaaaacgggcggaccgttgatatgtacgcgtttctattca
acgacatgtttgtgctgactaaaactaaaaagtgcgcttcgaagctgaaagtgaaaggag
tcccaatcgggaaatccgagcactacatcgtccaacgacagccggtaccgttggattctt
gcgtgttctgtgatgcggactccaacgaccaggcaacgccgatgtccctaaaattcgcgt
tcgtcataattcacctaacccgctactaccaagtcgtcggcatctacaccctacaagccg
ccgacaaggcggacaaggagctgtggatcgaaaagttccaggaatcgatcgaaaactacg
agtcgatacagttgaaggacatgctcaagtgtacaccgctcttctccagtcttagtttga
gaagatgctccagctcccgtctaatgtcgaaatccgagtcgaattctccgcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTTATCCCGTCAATTCTGCGAGAA 261 269 1379 Antisense
TCATCCGCGTCGAAAGCTTATCCAC 525 621 1413 Antisense
GGAAATCCGAGCACTACATCGTCCA 433 527 1568 Antisense
GGTACCGTTGGATTCTTGCGTGTTC 187 505 1602 Antisense
TGTTCTGTGATGCGGACTCCAACGA 7 561 1622 Antisense
TTCGCGTTCGTCATAATTCACCTAA 518 695 1672 Antisense
TACTACCAAGTCGTCGGCATCTACA 261 645 1702 Antisense
GACATGCTCAAGTGTACACCGCTCT 496 369 1816 Antisense
CGCTCTTCTCCAGTCTTAGTTTGAG 500 255 1835 Antisense
AGCTCCCGTCTAATGTCGAAATCCG 687 79 1870 Antisense
GAAATCCGAGTCGAATTCTCCGCAG 104 363 1887 Antisense
Sequence Source Affymetrix Proprietary Database