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[191723_s_at] WNT-2 precursor

Description W01B6.1 /REP_DB=WormBase Gene ID /WP=CE03753 /GEN=cwn-2 /TR=SW:P34889 /GB=CAA92624.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=WNT-2 precursor
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 27      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 90      6grp_total > 27      4grp_total > 30      6grp_highest_sub > Cu      6grp_cgc > 15      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 111
1 2 3 4 range
A 217.6
(3)
P 		198.4
(1.5)
P 		139.7
(-3)
P 		176.8
(-0.1)
P 		78
B 229.6
(3)
P 		160.4
(-3)
P 		174.9
(-1.7)
P 		197.3
(0.2)
P 		69
C 206
(1.9)
P 		118.7
(-3)
P 		124.3
(-2.7)
P 		225.9
(3)
P 		107
avr 217.7
(3) 		159.2
(-1.9) 		146.3
(-3) 		200
(1.5) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 153
1 2 3 4 5 6 range
A 215.8
(3)
P 		155.5
(-0.2)
P 		147.5
(-0.6)
P 		131.4
(-1.5)
P 		103.3
(-3)
P 		132.8
(-1.4)
P 		113
B 228.1
(3)
P 		165.1
(-0.2)
P 		165.9
(-0.1)
P 		109.6
(-3)
P 		136.2
(-1.7)
P 		160.2
(-0.4)
P 		119
C 209.3
(3)
P 		209.4
(3)
P 		114.5
(-1.2)
P 		75.4
(-3)
P 		76
(-3)
P 		144.2
(0.1)
P 		134
avr 217.7
(3) 		176.7
(0.8) 		142.6
(-1) 		105.5
(-3) 		105.2
(-3) 		145.7
(-0.8) 		113
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 79.2
(3)
P 		43.1
(-1.1)
P 		36.5
(-1.8)
P 		25.9
(-3)
P 		53
B 73.2
(3)
P 		39.6
(-3)
P 		40.5
(-2.8)
P 		42
(-2.6)
P 		34
C 68.2
(3)
P 		34.1
(-3)
P 		43.3
(-1.4)
P 		38.1
(-2.3)
P 		34
avr 73.5
(3) 		38.9
(-2.4) 		40.1
(-2.2) 		35.3
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 39.8
(-0.9)
P 		55.3
(3)
P 		32.1
(-2.8)
P 		33.3
(-2.5)
P 		31.2
(-3)
P 		32.3
(-2.7)
P 		24
B 79.4
(3)
P 		55
(0.1)
P 		40.3
(-1.7)
P 		29
(-3)
P 		55.3
(0.1)
P 		59.7
(0.7)
P 		50
C 77.6
(3)
P 		63.9
(1.7)
P 		29.1
(-1.7)
P 		15.2
(-3)
P 		24.6
(-2.1)
P 		48.3
(0.2)
P 		62
avr 65.6
(3) 		58.1
(1.9) 		33.8
(-1.8) 		25.8
(-3) 		37
(-1.3) 		46.8
(0.2) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191723_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10193
Sequence Type Exemplar sequence
Representative Public ID W01B6.1 NCBI
Target Description W01B6.1 /REP_DB=WormBase Gene ID /WP=CE03753 /GEN=cwn-2 /TR=SW:P34889 /GB=CAA92624.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=WNT-2 precursor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069421(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069421 NCBI Caenorhabditis elegans C. elegans WNT family member (cwn-2) (cwn-2) mRNA, complete cds. 11/11 None
SNAP00000040426 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10067847:10069938:1 11/11 None
GENEFINDER00000040441 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10068296:10069938:1 11/11 None
W01B6.1 ENSEMBL cdna:known chromosome:CEL140:IV:10067793:10070219:1 gene:W01B6.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10067852-10069944(+) 100.0 100.0
Public Domain and Genome References
Gene Title WNT-2 precursor
Gene Symbol cwn-2
Ensembl W01B6.1
Entrez Gene 177870 Entrez gene
SwissProt P34889 EMBL-EBI
WormBase CE03753 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1635852_AT Wnt oncogene analog 5 dm
DROSGENOME1:142877_AT Wnt oncogene analog 5 dm
CHICKEN:GGA.7960.1.S1_AT similar to Wnt-5b protein - mouse gga
HG-U133_PLUS_2:221029_S_AT wingless-type MMTV integration site family, member 5B hs
HG-U133A:221029_S_AT wingless-type MMTV integration site family, member 5B hs
HG-FOCUS:221029_S_AT wingless-type MMTV integration site family, member 5B hs
HG-U133A_2:221029_S_AT wingless-type MMTV integration site family, member 5B hs
HU35KSUBC:RC_AA599463_AT wingless-type MMTV integration site family, member 5B hs
U133_X3P:G13540540_3P_S_AT wingless-type MMTV integration site family, member 5B hs
U133_X3P:HS.306051.1.A1_3P_S_AT wingless-type MMTV integration site family, member 5B hs
HG-U133_PLUS_2:223537_S_AT wingless-type MMTV integration site family, member 5B hs
HG-U133B:223537_S_AT wingless-type MMTV integration site family, member 5B hs
HG-U95C:58787_AT wingless-type MMTV integration site family, member 5B hs
HG-U95C:61292_S_AT wingless-type MMTV integration site family, member 5B hs
HG-U95C:61330_AT wingless-type MMTV integration site family, member 5B hs
HG-U95C:66142_S_AT wingless-type MMTV integration site family, member 5B hs
HG-U95E:88133_AT wingless-type MMTV integration site family, member 5B hs
HG-U133_PLUS_2:230299_S_AT Wingless-type MMTV integration site family, member 5B hs
HG-U133B:230299_S_AT Wingless-type MMTV integration site family, member 5B hs
HG-U95C:59791_S_AT Wingless-type MMTV integration site family, member 5B hs
HG-U95E:90270_AT Wingless-type MMTV integration site family, member 5B hs
U133_X3P:HS.306051.2.A1_3P_AT Wingless-type MMTV integration site family, member 5B hs
MU11KSUBA:M89799_S_AT wingless-related MMTV integration site 5B mm
MG-U74AV2:103513_AT wingless-related MMTV integration site 5B mm
MG-U74CV2:171380_F_AT wingless-related MMTV integration site 5B mm
MG-U74CV2:169272_I_AT wingless-related MMTV integration site 5B mm
MG-U74CV2:169217_I_AT wingless-related MMTV integration site 5B mm
MG-U74CV2:169190_F_AT wingless-related MMTV integration site 5B mm
MOE430A:1422602_A_AT wingless-related MMTV integration site 5B mm
MOUSE430A_2:1422602_A_AT wingless-related MMTV integration site 5B mm
MOUSE430_2:1422602_A_AT wingless-related MMTV integration site 5B mm
MOE430A:1439373_X_AT wingless-related MMTV integration site 5B mm
MOUSE430A_2:1439373_X_AT wingless-related MMTV integration site 5B mm
MOUSE430_2:1439373_X_AT wingless-related MMTV integration site 5B mm
MG-U74CV2:171408_F_AT Wingless-related MMTV integration site 5B, mRNA (cDNA clone MGC:18677 IMAGE:4211437) mm
MU19KSUBB:TC26577_AT Wingless-related MMTV integration site 5B, mRNA (cDNA clone MGC:18677 IMAGE:4211437) mm
RAE230A:1374970_AT wingless-related MMTV integration site 5B rn
RAT230_2:1374970_AT wingless-related MMTV integration site 5B rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7223 frizzled-2 signaling pathway inferred from electronic annotation QuickGO AmiGO
7275 development inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4871 signal transducer activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92624 Hypothetical protein W01B6.1 [Caenorhabditis elegans] ref|NP_501822.1| C. elegans WNT family member (cwn-2) [Caenorhabditis elegans] 0.0
blast P34889 Wnt-2 protein precursor emb|CAA51448.1| Wnt-2 protein [Caenorhabditis elegans] 0.0
blast CAE59936 Hypothetical protein CBG03422 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005817 EMBL-EBI Wnt superfamily 1.0E-126
Pfam IPR005817 EMBL-EBI Wnt superfamily 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191723_S_AT
tggagttggggtggatgtggagataatgttgaatatggttacaagttttcgagagatttt
attgatattcgagagaaagaacacgatccaaagagaaatcacgataacggtagaagttta
atgaatcgaagaaataacgaagctggaagaaaaattttgaaacgtcatcgaaaaccaaaa
tgcaaatgccatggagtttcgggtgcgtgcaacatgaaaacctgttggatgcaacttcct
tcgatggaacaagtcggaaaaatcttgaggaataaatatgacaaagcaatcagagttcag
atcaatgatagaggaaatttgcaacttctagcagatgaagctacaaaagaaagaaaaaca
agagcattgccaacggatttagtatttatggatgattcacctgattactgtagatttgat
cgacattctggaacgttaggaactgaaggaagggtttgcaaacgtggctcgggtggagct
gaaggctgtgatagcttgtgctgtggacgaggatat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGAGTTGGGGTGGATGTGGAGATA 549 547 469 Antisense
AAATGCCATGGAGTTTCGGGTGCGT 396 119 652 Antisense
GGGTGCGTGCAACATGAAAACCTGT 171 491 669 Antisense
GTTGGATGCAACTTCCTTCGATGGA 291 437 692 Antisense
GGAAATTTGCAACTTCTAGCAGATG 174 527 781 Antisense
GAGCATTGCCAACGGATTTAGTATT 20 389 830 Antisense
GGATGATTCACCTGATTACTGTAGA 245 509 858 Antisense
TGATCGACATTCTGGAACGTTAGGA 6 481 885 Antisense
GTGGCTCGGGTGGAGCTGAAGGCTG 13 491 932 Antisense
GAAGGCTGTGATAGCTTGTGCTGTG 597 333 949 Antisense
TAGCTTGTGCTGTGGACGAGGATAT 90 649 960 Antisense
Sequence Source Affymetrix Proprietary Database