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[191719_at] enoyl CoA hydratase/isomerase

Description R06F6.9 /REP_DB=WormBase Gene ID /WP=CE01625 /TR=Q09603 /GB=CAA86779.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=enoyl CoA hydratase-isomerase
Cluster 6grp_highest_10_10 > 04      6grp_high1_10_10 > 40      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 47      6grp_total > 53      4grp_total > 32      6grp_cgc > 90      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1214
1 2 3 4 range
A 2583.3
(-3)
P 		2786.2
(-1.2)
P 		3276.5
(3)
P 		3036
(0.9)
P 		693
B 2451.2
(-3)
P 		2959.7
(1.2)
P 		3173
(3)
P 		2760.1
(-0.4)
P 		722
C 2062.9
(-3)
P 		2525.8
(-0.1)
P 		2788.8
(1.6)
P 		3005.4
(3)
P 		943
avr 2365.8
(-3) 		2757.2
(0.3) 		3079.4
(3) 		2933.8
(1.8) 		714

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1878
1 2 3 4 5 6 range
A 3605
(2.3)
P 		3217.8
(-1.6)
P 		3630.5
(2.6)
P 		3668.7
(3)
P 		3081.6
(-3)
P 		3180.2
(-2)
P 		587
B 3374.9
(1.3)
P 		2632.1
(-3)
P 		3275.5
(0.7)
P 		3667
(3)
P 		3668.6
(3)
P 		2704.8
(-2.6)
P 		1037
C 3306.9
(0)
P 		2808.6
(-1.5)
P 		4230.7
(3)
P 		2352.3
(-3)
P 		3625.9
(1.1)
P 		2496.4
(-2.5)
P 		1878
avr 3428.9
(1.1) 		2886.2
(-2.4) 		3712.2
(3) 		3229.3
(-0.2) 		3458.7
(1.3) 		2793.8
(-3) 		918
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 528
1 2 3 4 range
A 940.6
(3)
P 		604.8
(-1.1)
P 		856.5
(2)
P 		444.4
(-3)
P 		496
B 781.7
(3)
P 		731
(1.4)
P 		735.3
(1.6)
P 		588.2
(-3)
P 		194
C 682.9
(-0.7)
P 		726.2
(-0.2)
P 		972
(3)
P 		506.8
(-3)
P 		465
avr 801.7
(2.1) 		687.3
(0.1) 		854.6
(3) 		513.1
(-3) 		342

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1017
1 2 3 4 5 6 range
A 664.6
(-3)
P 		1144.4
(3)
P 		790.6
(-1.4)
P 		930.5
(0.3)
P 		929.9
(0.3)
P 		774.3
(-1.6)
P 		480
B 1174.3
(0.3)
P 		876.1
(-2.3)
P 		795.4
(-3)
P 		971.3
(-1.5)
P 		1490.5
(3)
P 		1008.6
(-1.2)
P 		695
C 1225.7
(3)
P 		857
(0.1)
P 		1076.8
(1.8)
P 		473.1
(-3)
P 		1173.3
(2.6)
P 		835.7
(-0.1)
P 		753
avr 1021.5
(0.4) 		959.2
(-0.5) 		887.6
(-1.6) 		791.6
(-3) 		1197.9
(3) 		872.9
(-1.8) 		406
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191719_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3183
Sequence Type Exemplar sequence
Representative Public ID R06F6.9 NCBI
Target Description R06F6.9 /REP_DB=WormBase Gene ID /WP=CE01625 /TR=Q09603 /GB=CAA86779.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=enoyl CoA hydratase-isomerase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063929(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063929 NCBI Caenorhabditis elegans Enoyl-CoA Hydratase family member (ech-4) (ech-4) mRNA, complete cds. 11/11 None
SNAP00000015435 ENSEMBL cdna:SNAP chromosome:CEL140:II:10820943:10822193:1 11/11 None
GENEFINDER00000015449 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:10820943:10822193:1 11/11 None
R06F6.9 ENSEMBL cdna:known chromosome:CEL140:II:10820942:10822282:1 gene:R06F6.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10820939-10822190(+) 100.0 100.0
Public Domain and Genome References
Gene Title enoyl CoA hydratase/isomerase
Gene Symbol ech-4
Ensembl R06F6.9
Entrez Gene 174665 Entrez gene
SwissProt Q09603 EMBL-EBI
WormBase CE01625 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604937_AT similar to peroxisomal D3,D2-enoyl-CoA isomerase isoform 1 cfa
CANINE:1591650_S_AT similar to peroxisomal D3,D2-enoyl-CoA isomerase isoform 1 cfa
CANINE_2:CFA.2938.1.S1_AT similar to peroxisomal D3,D2-enoyl-CoA isomerase isoform 1 cfa
CANINE_2:CFAAFFX.14836.1.S1_S_AT similar to peroxisomal D3,D2-enoyl-CoA isomerase isoform 1 cfa
CHICKEN:GGA.11390.1.S1_AT peroxisomal D3,D2-enoyl-CoA isomerase gga
HG-U133_PLUS_2:218025_S_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
HG-U133A:218025_S_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
HG-FOCUS:218025_S_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
HG-U133A_2:218025_S_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
HU35KSUBA:W79046_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
HU35KSUBA:RC_AA620556_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
HU35KSUBA:W28824_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
U133_X3P:G5174624_3P_S_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
HG-U95B:44706_AT peroxisomal D3,D2-enoyl-CoA isomerase hs
MOUSE430_2:1431012_A_AT peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase mm
MOE430A:1431012_A_AT peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase mm
MOUSE430A_2:1431012_A_AT peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase mm
MG-U74AV2:94485_AT peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase mm
MU19KSUBB:TC26647_S_AT Peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase, mRNA (cDNA clone MGC:5644 IMAGE:3591615) mm
MU19KSUBB:TC31690_AT Peroxisomal delta3, delta2-enoyl-Coenzyme A isomerase, mRNA (cDNA clone MGC:5644 IMAGE:3591615) mm
RG-U34C:RC_AI104482_AT similar to Peci protein rn
RAE230A:1388908_AT similar to Peci protein rn
RAT230_2:1388908_AT similar to Peci protein rn
RAE230A:1372765_A_AT similar to Peci protein rn
RAT230_2:1372765_A_AT similar to Peci protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
62 acyl-CoA binding inferred from electronic annotation QuickGO AmiGO
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86779 Hypothetical protein R06F6.9 [Caenorhabditis elegans] ref|NP_496330.1| Enoyl-CoA Hydratase family member (ech-4) [Caenorhabditis elegans] 0.0
blast CAE59601 Hypothetical protein CBG03009 [Caenorhabditis briggsae] 0.0
blast XP_418965 PREDICTED: similar to hypothetical protein [Gallus gallus] 5.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR000582 EMBL-EBI Acyl-coA-binding protein, ACBP 6.8E-52
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 7.9E-20
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 6.1E-5
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 2.2E-14
Sequence
Target Sequence 
>C. ELEGANS:191719_AT
gagaagcctttgattgctctgatcaatggcccagccgtaggaattgctgtgaccgtgctc
ggaatgttcgactacgtaattgcaaccgataaggcctcattccacactccatttgcccca
cttggacaatcacctgaaggagtttcgagctacacgttcccactgatcatgggatctttg
agagcttctgagatgctgttggtctgtaagaaaatctcagcccaaactgcaaaggattac
gggcttgtgaatgaagttgtgccagacgctgaattccagtcacatgctcaaaaaactgtg
gaagcgttctcccaacttccgccagaaactcttcgtatcaataagaaattgctccgttct
ctgcacaaggagaagcttctggaggtcaacaatatcgaggccgaccagatttgcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAAGCCTTTGATTGCTCTGATCA 630 391 688 Antisense
CTCTGATCAATGGCCCAGCCGTAGG 208 231 704 Antisense
GTAGGAATTGCTGTGACCGTGCTCG 5 457 724 Antisense
GACCGTGCTCGGAATGTTCGACTAC 95 383 738 Antisense
TTGCAACCGATAAGGCCTCATTCCA 357 703 767 Antisense
CCCCACTTGGACAATCACCTGAAGG 163 261 803 Antisense
GAAGGAGTTTCGAGCTACACGTTCC 586 331 823 Antisense
GTGCCAGACGCTGAATTCCAGTCAC 145 479 946 Antisense
TTCCGCCAGAAACTCTTCGTATCAA 452 697 1004 Antisense
ATTGCTCCGTTCTCTGCACAAGGAG 582 3 1035 Antisense
AATATCGAGGCCGACCAGATTTGCG 271 173 1078 Antisense
Sequence Source Affymetrix Proprietary Database